CARD 6 CRISPR/Cas9 KO Plasmid (h): sc-413684
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CARD 6 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CARD 6 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARD 6 CRISPR/Cas9 KO Plasmid (h) | sc-413684 | 20 µg | $397.00 |
Overview
CARD6 (caspase recruitment domain family member 6) is a human CARD-containing adaptor protein implicated in pattern-recognition receptor signaling and modulation of inflammatory responses. Through CARD-mediated protein–protein interactions, CARD6 has been linked to regulation of NF-κB and MAPK pathway activity downstream of microbial sensing and stress cues, influencing cytokine output and innate immune activation. Altered CARD6 signaling has been discussed in the context of dysregulated inflammation and immune-associated pathophysiology, making it relevant for mechanistic studies of host defense and inflammatory signaling networks.
CARD 6 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CARD6 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CARD6 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CARD6 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CARD 6 protein expression.
This CRISPR knockout system enables efficient generation of CARD6-deficient cell models for investigation of CARD 6 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CARD6 exon(s) critical for CARD 6 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CARD6 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CARD 6 CRISPR/Cas9 KO Plasmid (h) and CARD 6 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CARD6 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CARD 6 HDR Plasmid (h) and CARD 6 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CARD6 homology arms to support homology-directed repair at defined CARD6 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.