CAP2抗体 (A-5) 推荐用于 mouse, rat 和 human 来源的CAP2 WB, IP, IF 和 ELISA检测

CAP2 抗体 (A-5): sc-377471

CAP2抗体 (A-5) is rated 5.0 out of 5 by 3.
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说明书

    • 抗-CAP2 抗体 A-5 是小鼠单克隆 IgG1 κ,CAP2抗体, 在1篇文献中引用,规格为200 µg/ml
    • 免疫原氨基酸序列77-121位于human物种的CAP2的N-terminus附近
    • 抗-CAP2 抗体 (A-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CAP2
    • 抗-CAP2 抗体 (A-5) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CAP2 (A-5): sc-377471免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 对于CAP2 抗体 (A-5)的WB应用,m-IgGκ BP-HRP 是首选的二抗检测试剂。现在该试剂和CAP2 抗体 (A-5)组合成套装提供(参见下面的订购信息)。对于其他m-IgGκ BP 偶联产品,参见我们小鼠IgG 结合蛋白的完整列表
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What is the recommended fixation method for immunofluorescence staining with CAP2 (A-5): sc-377471 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 5 out of 5 by from nice product! dilution rate 1:300 for WB can get a clear and single band in SW480,SW48,HCT116,HT29,LOVO,DLD1.
Date published: 2017-09-12
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmic Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-03
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