Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

Calpain reg 抗体 (P-1): sc-32325

4.0(2)
寫評論提問

说明书
  • Calpain reg 抗体 P-1 是小鼠单克隆 IgG1 κ,Calpain reg抗体, 在5篇文献中引用,规格为200 µg/ml
  • 针对牛心肌小调节亚基复合钙蛋白酶2
  • Calpain reg 抗体 (P-1) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 Calpain reg; 与large calpain catalytic subunits不交叉反应
  • 抗Calpain reg抗体(P-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Calpain reg (P-1): sc-32325免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Calpain reg Antibody (P-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Calpain reg Antibody (P-1) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

钙蛋白酶抑制剂抗体(P-1)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人类来源的钙蛋白酶抑制剂蛋白。抗钙蛋白酶调节蛋白抗体(P-1)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。钙蛋白酶调节蛋白作为钙蛋白酶家族中钙依赖性半胱氨酸蛋白酶的调节亚基,在调节特定的钙依赖性细胞过程(如细胞融合、有丝分裂和减数分裂)中起着至关重要的作用。这些蛋白酶是由一个小的调节亚基(钙蛋白酶调节蛋白)和三个大的催化亚基(钙蛋白酶1、钙蛋白酶2或钙蛋白酶p94)之一组成的异二聚体。钙蛋白酶活性的调节受钙蛋白抑制素的影响,后者可抑制钙蛋白酶的蛋白水解活性及膜结合。这种调节机制在多种生理过程中尤为重要,包括精子发生和受精前的顶体反应。此外,钙蛋白酶1与人类白细胞抗原-DR(HLA-DR)在衰老大脑中活化的微胶质细胞上共定位,这表明钙蛋白酶1可能参与神经炎症反应。钙蛋白酶调节蛋白(P-1)抗体能够有效检测和鉴定钙蛋白酶调节蛋白,是研究钙蛋白酶在细胞信号传导和病理学中复杂作用的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Calpain reg 抗体 (P-1) 参考文献:

  1. 心肌梗死中钙蛋白酶的活化:使用针对活性位点含组氨酸肽的钙蛋白酶抗体进行的免疫组化研究。  |  Kunimatsu, M., et al. 1999. Cardiovasc Pathol. 8: 7-15. PMID: 10722243
  2. 红细胞钙蛋白的分子多样性。  |  Takano, E., et al. 1991. Biomed Biochim Acta. 50: 517-21. PMID: 1801717
  3. 钙蛋白酶-1促进IgE介导的肥大细胞活化。  |  Wu, Z., et al. 2014. J Immunol. 192: 5130-9. PMID: 24760147
  4. 细胞外 CIRP 通过 PLC-IP3 依赖性钙通路诱导神经元中的钙蛋白酶活化  |  Sharma, A., et al. 2023. Mol Neurobiol. 60: 3311-3328. PMID: 36853429
  5. 钙依赖性钙蛋白酶对局灶粘附激酶的激活参与了慢性淋巴细胞白血病细胞的侵袭性。  |  Severin, F., et al. 2023. Br J Haematol. 203: 224-236. PMID: 37495265
  6. 针对钙蛋白酶生成的 ∆48 kDa 降钙素原片段开发特异性单克隆抗体,该片段是受损星形胶质细胞的标志物。  |  Kraner, SD., et al. 2024. J Neurosci Methods. 402: 110012. PMID: 37984591
  7. 抗体共轭靶向纳米疗法从表观遗传学角度抑制钙蛋白酶介导的线粒体功能障碍,从而减轻帕金森病的病情。  |  Biswal, L., et al. 2024. Carbohydr Polym. 346: 122575. PMID: 39245478
  8. 钙依赖蛋白酶和特异性抑制剂:钙蛋白酶和钙司他丁。  |  Murachi, T. 1984. Biochem Soc Symp. 49: 149-67. PMID: 6100833
  9. 人类红细胞中钙磷脂的分子多样性。  |  Takano, E., et al. 1993. Arch Biochem Biophys. 303: 349-54. PMID: 8512320
  10. m-calpain 的自溶, Ca2+ 需求和异二聚体稳定性。  |  Elce, JS., et al. 1997. J Biol Chem. 272: 11268-75. PMID: 9111030
  11. 膜对钙蛋白酶-钙司他丁系统的调控(综述)。  |  Kawasaki, H. and Kawashima, S. 1996. Mol Membr Biol. 13: 217-24. PMID: 9116760
  12. 钙蛋白酶:完整而活跃?  |  Johnson, GV. and Guttmann, RP. 1997. Bioessays. 19: 1011-8. PMID: 9394623

订购信息

产品名称产品编号规格价格数量收藏夹

Calpain reg 抗体 (P-1)

sc-32325
200 µg/ml
$316.00

Calpain reg (P-1): m-IgG Fc BP-HRP 套装

sc-528464
200 µg Ab; 10 µg BP
$354.00

Calpain reg (P-1): m-IgGκ BP-HRP 套装

sc-520847
200 µg Ab, 40 µg BP
$354.00

Calpain reg (P-1): m-IgG1 BP-HRP 套装

sc-542944
200 µg Ab; 20 µg BP
$354.00

Calpain reg 抗体 (P-1) AC

sc-32325 AC
500 µg/ml, 25% agarose
$416.00

Calpain reg 抗体 (P-1) HRP

sc-32325 HRP
200 µg/ml
$316.00

Calpain reg 抗体 (P-1) FITC

sc-32325 FITC
200 µg/ml
$330.00

Calpain reg 抗体 (P-1) PE

sc-32325 PE
200 µg/ml
$343.00

Calpain reg 抗体 (P-1) Alexa Fluor® 488

sc-32325 AF488
200 µg/ml
$357.00

Calpain reg 抗体 (P-1) Alexa Fluor® 546

sc-32325 AF546
200 µg/ml
$357.00

Calpain reg 抗体 (P-1) Alexa Fluor® 594

sc-32325 AF594
200 µg/ml
$357.00

Calpain reg 抗体 (P-1) Alexa Fluor® 647

sc-32325 AF647
200 µg/ml
$357.00

Calpain reg 抗体 (P-1) Alexa Fluor® 680

sc-32325 AF680
200 µg/ml
$357.00

Calpain reg 抗体 (P-1) Alexa Fluor® 790

sc-32325 AF790
200 µg/ml
$357.00

If I use this antibody coupled to agarose beads for IP, does the light chains appears on the western blot  where I want to reveal the immunoprecipitated CAPNS1 (same size as the light chain) ? Thanks for your response  

Asked by: cordo
Thank you for your question. The agarose conjugated antibody (sc-32325 AC) is covalently bound to sepharose (agarose) beads. This will greatly reduce the amount of IP antibody (heavy and light chains) which might be detected in the sample by Western blot. However, cross-linking of the IgG to the agarose will never be 100% and some antibodies are not cross-linked and may come off under elution or when using reducing agents in the sample buffer before gel loading. One way to avoid detecting the light chain IgG in your Western blot is to use the primary antibody directly conjugated to HRP (sc-32325 HRP) for direct Western blot of your immunoprecipitated samples. Then it will not be necessary to use anti-mouse IgG secondary antibody for detection.
Answered by: Technical Support
Date published: 2020-07-08

Are there any issues with using sc-32325: Calpain reg (P-1) mouse monoclonal primary antibody on mouse samples?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent,we recommend using a directly conjugated primary antibody. Please contact our special orders department for pricing and availability of sc-32325 conjugates.
Answered by: Technical Support
Date published: 2017-03-07
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_32325, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 150ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-04-24
Rated 4 out of 5 by from Robust bands seen in WB using human PBLRobust bands seen in WB using human PBL, TF-1 and K-562 whole cell lysates. -SCBT QC
Date published: 2014-03-01
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_32325, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT
Calpain reg 抗体 (P-1) is rated 4.0 out of 5 by 2.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_2
  • loc_zh_CN, sid_32325, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 105ms
  • REVIEWS, PRODUCT