Date published: 2025-9-7
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Yes Antibody (C-10) - Western Blotting - Image 358454
c-Yes 抗体 (C-10): sc-46674. SW480 (A), A-431 (B), NIH/3T3 (C), Sol8 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 c-Yes 表达的 Western 印迹分析.
c-Yes 抗体 (C-10): sc-46674. SW480 (A), A-431 (B), NIH/3T3 (C), Sol8 (D), KNRK (E) 和 L8 (F) 全细胞裂解液中 c-Yes 表达的 Western 印迹分析.

Yes 抗体 (C-10): sc-46674

5.0(10)
寫評論提問

说明书
  • Yes 抗体 C-10 是小鼠单克隆 IgG1, 在6篇文献中引用，提供 200 µg/ml
  • 免疫human物种的c-Yes的氨基酸1-95
  • 抗-Yes 抗体 (C-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 c-Yes p61
  • 抗Yes抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Yes (C-10): sc-46674的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 Yes 抗体 (C-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Yes 抗体 (C-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

c-Yes抗体（C-10）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的c-Yes蛋白。c-Yes（C-10）抗体 非结合和多种结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。c-Yes蛋白在细胞信号传导通路中发挥着关键作用，特别是在调节细胞增殖和分化方面，这使得c-Yes成为癌症研究的重要靶标。作为Src家族激酶的成员，c-Yes（C-10）单克隆抗体可识别一种参与信号转导的蛋白质，该信号可影响包括运动和粘附在内的各种细胞过程。c-Yes蛋白主要位于细胞质中，并可转移到细胞膜，在细胞膜上与其他信号分子相互作用，从而调节其活性和功能。人类c-Yes基因位于18p11.31-p11.21染色体上，编码一种543个氨基酸的蛋白质，与c-Src有80%以上的同源性，这凸显了这些激酶在进化中的保守性和功能重要性。了解c-Yes的动态变化及其与特定蛋白的相互作用，有助于深入了解c-Yes在致癌过程中的作用以及癌症治疗中的潜在治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Yes 抗体 (C-10) 参考文献:

  1. SH3 和 SH2 结构域能够引导非受体酪氨酸激酶 cSrc 和 cYes 之间蛋白质相互作用的特异性。  |  Summy, JM., et al. 2000. Oncogene. 19: 155-60. PMID: 10644991
  2. Src 家族激酶：结构与功能。  |  Tatosyan, AG. and Mizenina, OA. 2000. Biochemistry (Mosc). 65: 49-58. PMID: 10702640
  3. 白细胞中 Src 激酶介导的信号传导。  |  Korade-Mirnics, Z. and Corey, SJ. 2000. J Leukoc Biol. 68: 603-13. PMID: 11073097
  4. Src 激酶活化和功能的精选一瞥。  |  Bjorge, JD., et al. 2000. Oncogene. 19: 5620-35. PMID: 11114743
  5. SRC家族激酶介导内皮素对上皮Na+通道的抑制作用  |  Gilmore, ES., et al. 2001. J Biol Chem. 276: 42610-7. PMID: 11560932
  6. c-Yes 酪氨酸激酶是 ES 细胞分化的强效抑制因子，能拮抗其系统发育近亲 c-Src 的作用。  |  Zhang, X., et al. 2014. ACS Chem Biol. 9: 139-46. PMID: 23895624
  7. 使用计算机模拟和实验技术鉴定c-Yes激酶的I型和II型抑制剂。  |  Ramakrishnan, C., et al. 2018. J Biomol Struct Dyn. 36: 1566-1576. PMID: 28589758
  8. c-yes 基因在人类染色体上的位置及其在不同组织中的表达。  |  Semba, K., et al. 1985. Science. 227: 1038-40. PMID: 2983418
  9. 人类结肠癌中的c-Yes酪氨酸激酶活性。  |  Park, J., et al. 1993. Oncogene. 8: 2627-35. PMID: 7690925
  10. 结肠癌前病变中c-yes酪氨酸激酶活性升高。  |  Peña, SV., et al. 1995. Gastroenterology. 108: 117-24. PMID: 7806032
  11. 深入了解 Src 激酶的功能：结构比较。  |  Williams, JC., et al. 1998. Trends Biochem Sci. 23: 179-84. PMID: 9612082
  12. 人类原癌基因的基因组织。  |  Sakaguchi, AY. 1983. Prog Clin Biol Res. 119: 93-103. PMID: 6191340

订购信息

产品名称产品编号规格价格数量收藏夹

Yes 抗体 (C-10)

sc-46674
200 µg/ml
$316.00

Yes (C-10): m-IgG Fc BP-HRP 套装

sc-525548
200 µg Ab; 10 µg BP
$354.00

Yes 抗体 (C-10) AC

sc-46674 AC
500 µg/ml, 25% agarose
$416.00

Yes 抗体 (C-10) HRP

sc-46674 HRP
200 µg/ml
$316.00

Yes 抗体 (C-10) FITC

sc-46674 FITC
200 µg/ml
$330.00

Yes 抗体 (C-10) PE

sc-46674 PE
200 µg/ml
$343.00

Yes 抗体 (C-10) Alexa Fluor® 488

sc-46674 AF488
200 µg/ml
$357.00

Yes 抗体 (C-10) Alexa Fluor® 546

sc-46674 AF546
200 µg/ml
$357.00

Yes 抗体 (C-10) Alexa Fluor® 594

sc-46674 AF594
200 µg/ml
$357.00

Yes 抗体 (C-10) Alexa Fluor® 647

sc-46674 AF647
200 µg/ml
$357.00

Yes 抗体 (C-10) Alexa Fluor® 680

sc-46674 AF680
200 µg/ml
$357.00

Yes 抗体 (C-10) Alexa Fluor® 790

sc-46674 AF790
200 µg/ml
$357.00

c-Yes (C-10)和c-Yes (F-7)有什么区别？

Asked by: Wqiwen
感谢您的提问。这两只抗体的抗原表位不同，可应用的实验类型和样本种属相同。 Thank you for your question. The two antibodies have different epitope sequences, but they can be used in the same application and sample species.
Answered by: Technical Service SH
Date published: 2022-11-08

I am using c-Yes (C-10): sc-46674 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from specific/sharp bands detectedThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
Rated 5 out of 5 by from Anti-cYES antibodyMouse anti-c-YES antibody (sc-46674) works great on immunoblotting at 1:1000 in 2.5 % milk powder. It also works great on IP at 1ug per 1 ml of cell lyaste or 300 ug of tissue lysate.
Date published: 2017-08-24
Rated 5 out of 5 by from Very good antibodyI used this antibody for my IP WB on human samples, and it gave me strong band!
Date published: 2017-03-30
Rated 5 out of 5 by from Great choice for WB!This monoclonal c-Yes Antibody (C-10) gives excellent results in Western blotting in samples including, SW480, A-431, NIH/3T3, Sol8, KNRK and L8 whole cell lysates.
Date published: 2017-02-08
Rated 5 out of 5 by from Excellent antibody for Western blottingThis product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It strongly detected an appropriately sized 55kDa protein in these immunoblots, which is the expect migration position for Yes on SDS-PAGE gels, although the predicted molecular mass for Yes is ~60.8 kDa.
Date published: 2016-10-13
Rated 5 out of 5 by from Clean bands observed in western blot analysis of cClean bands observed in western blot analysis of c-Yes expression in Jurkat and CTLL-2 whole cell lysates. -SCBT QC
Date published: 2015-07-03
Rated 5 out of 5 by from Superb immunoperoxidase cytoplasmic stainingSuperb immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human colon tissue. -SCBT QC
Date published: 2015-04-26
Rated 5 out of 5 by from Successfully immunoprecipitated cSuccessfully immunoprecipitated c-Yes protein from LN cells; verified with western blot analysis. -SCBT Publication Review
Date published: 2015-04-14
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Yes 抗体 (C-10) is rated 5.0 out of 5 by 10.
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