登陆账户

购物车 (0)

c-Mpl Antibody (E-7) - Western Blotting - Image 374519 — c-Mpl 抗体 (E-7): sc-377417. 拉莫斯 (A), Neuro-2A (B) 和 HeLa (C) 全细胞裂解液中 c-Mpl 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

c-Mpl 抗体 (E-7): sc-377417. 拉莫斯 (A), Neuro-2A (B) 和 HeLa (C) 全细胞裂解液中 c-Mpl 表达的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

c-Mpl 抗体 (E-7): sc-377417

Name: c-Mpl抗体 (E-7)
Brand: SCBT
SKU: sc-377417
Price: 316.0 USD
Availability: InStock

4.8(4)

寫評論提問

说明书

c-Mpl 抗体 E-7 是小鼠单克隆 IgM κ，c-Mpl抗体, 在1篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的c-Mpl的C-terminus附近的氨基酸531-569之间
推荐用于 mouse, rat 和 human 来源的c-Mpl WB, IP, IF 和 ELISA检测
关于如何获取c-Mpl (E-7): sc-377417的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgGκ BP-HRP是c-Mpl Antibody (E-7) 适用于 WB 应用。的首选辅助检测试剂。该试剂现可与c-Mpl Antibody (E-7) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

查看产品引用文献(1)

快捷链接

c-Mpl 抗体 (E-7) 参考文献:

c-Mpl 细胞内结构域的远半部在控制体内血小板生成过程中的作用。 | Luoh, SM., et al. 2000. Mol Cell Biol. 20: 507-15. PMID: 10611229
桡骨缺失血小板减少综合征中的 c-Mpl 信号缺陷 | Ballmaier, M., et al. 1998. Stem Cells. 16 Suppl 2: 177-84. PMID: 11012189
先天性无巨核细胞血小板减少症（CAMT）的血小板生成素受体（c-mpl 基因）中两个不同氨基酸替换突变的复合杂合性。 | Tonelli, R., et al. 2000. Hum Genet. 107: 225-33. PMID: 11071383
血小板生成素受体（c-Mpl）的C-甘露糖基化调节血小板生成素依赖性JAK-STAT信号传导。 | Sasazawa, Y., et al. Biochem Biophys Res Commun. 468: 262-8. PMID: 26505790
C-Cbl 调节 c-MPL 受体的运输和内化。 | Märklin, M., et al. 2020. J Cell Mol Med. 24: 12491-12503. PMID: 32954656
c-Mpl-del 是 c-Mpl 的另一种剪接形式，可促进 AMKL 进展和耐药性。 | Li, F., et al. 2022. Cell Death Dis. 13: 869. PMID: 36229456
PF4 激活血小板中的 c-Mpl-Jak2 途径。 | Buka, RJ., et al. 2024. Blood. 143: 64-69. PMID: 37883794
c-Mpl 配体--血小板生成素对体外血小板活化的调节作用 | Chen, J., et al. 1995. Blood. 86: 4054-62. PMID: 7492761
血小板生成素（TPO）诱导 STAT5 和 STAT3 的酪氨酸磷酸化和活化。 | Bacon, CM., et al. 1995. FEBS Lett. 370: 63-8. PMID: 7544303
TPO和IL-3在骨髓前体细胞系中诱导重叠但不同的蛋白酪氨酸磷酸化。 | Dorsch, M., et al. 1995. Biochem Biophys Res Commun. 214: 424-31. PMID: 7545904
c-Mpl 配体血小板生成素可诱导 Tyk2, JAK2 和 STAT3 的酪氨酸磷酸化，并增强体外激动剂诱导的血小板聚集。 | Ezumi, Y., et al. 1995. FEBS Lett. 374: 48-52. PMID: 7589510
c-mpl缺陷小鼠血小板减少。 | Gurney, AL., et al. 1994. Science. 265: 1445-7. PMID: 8073287

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
c-Mpl 抗体 (E-7)	sc-377417	200 µg/ml	$316.00
c-Mpl (E-7): m-IgGκ BP-HRP 套装	sc-523324	200 µg Ab, 40 µg BP	$354.00
c-Mpl (E-7) 中和勝肽	sc-377417 P	100 µg/0.5 ml	$68.00

If the antibody to c-Mpl E-7 is a monoclonal mouse Ig M isotype, why do you suggest to use m-IgGκ BP-HRP as a secondary reagent? Is the secondary able to bind IgM as well?

Asked by: simcopp

Thank you for your question. IgGκ BP-HRP is the preferred secondary detection reagent for c-Mpl Antibody (E-7) for WB applications but, yes an IgM secondary would work as well.

Answered by: BlakeJ

Date published: 2025-03-13

I am not getting the Western Blot for c-MPL. can you kindly describe the solution in which antibody dilution is made? Thank You

Asked by: amjad

Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Service

Date published: 2022-05-18

What application is the blocking peptide sc-377417 P appropriate for?

Asked by: Professor Griffin

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-24

Rated 4 out of 5 by RK_16 from c-MPLThe Antibody works great and efficiently detects WB, and IF Tried with both Adherent and non-adherent cells. However, we needed to use higher dilution then mentioned.

Date published: 2018-09-28

Rated 5 out of 5 by Germaine from Great WB antibodyProduced nice strong and clean bands in WB on Daudi, U266, LADMAC and M1 whole cell lysates in a 1:200 dilution.

Date published: 2017-02-08

Rated 5 out of 5 by michelles from TPO related function reearchI bought this antibody for the purification of c-Mpl by affinity chromatography.It have a high affinity to my target protein.

Date published: 2016-12-18

Rated 5 out of 5 by SS from Produced positive Western blot data of cProduced positive Western blot data of c-Mpl expression in Ramos and U266 whole cell lysates. -SCBT QC

Date published: 2014-05-03

c-Mpl 抗体 (E-7) is rated 4.8 out of 5 by 4.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	MPL	Entrez Gene ID: 4352	1p34.2	XM_017001320, NM_005373	UniProt ID: P40238	604498
Mouse	Mpl	Entrez Gene ID: 17480	4 D2.1	XM_011240468, NM_001122949, NM_001285496, NM_001285497, NM_010823	UniProt ID: Q08351	N/A

c-Mpl 抗体 (E-7): sc-377417

c-Mpl 抗体 (E-7) 参考文献:

c-Mpl 抗体 (E-7) 规格

替代产品名称: c-Mpl

产品克隆名称: E-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgM kappa light chain

氨基酸: 531-569

抗原表位靶标: c-Mpl

抗原表位种属: human

产品反应性/检测: c-Mpl

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 1篇各种科学出版物的引文

c-Mpl 抗体 (E-7)可选的偶联物/形式: P: Blocking Peptide

c-Mpl 抗体 (E-7)

c-Mpl (E-7): m-IgGκ BP-HRP 套装

c-Mpl (E-7) 中和勝肽

If the antibody to c-Mpl E-7 is a monoclonal mouse Ig M isotype, why do you suggest to use m-IgGκ BP-HRP as a secondary reagent? Is the secondary able to bind IgM as well?

I am not getting the Western Blot for c-MPL. can you kindly describe the solution in which antibody dilution is made? Thank You

What application is the blocking peptide sc-377417 P appropriate for?

c-Mpl 抗体 (E-7): sc-377417

c-Mpl 抗体 (E-7) 参考文献:

c-Mpl 抗体 (E-7) 规格

替代产品名称: c-Mpl

产品克隆名称: E-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgM kappa light chain

氨基酸: 531-569

抗原表位靶标: c-Mpl

抗原表位种属: human

产品反应性/检测: c-Mpl

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 1篇各种科学出版物的引文

c-Mpl 抗体 (E-7)可选的偶联物/形式: P: Blocking Peptide

c-Mpl 抗体 (E-7)

c-Mpl (E-7): m-IgGκ BP-HRP 套装

c-Mpl (E-7) 中和勝肽

If the antibody to c-Mpl E-7 is a monoclonal mouse Ig M isotype, why do you suggest to use m-IgG&kappa; BP-HRP as a secondary reagent? Is the secondary able to bind IgM as well?

I am not getting the Western Blot for c-MPL. can you kindly describe the solution in which antibody dilution is made? Thank You

What application is the blocking peptide sc-377417 P appropriate for?

If the antibody to c-Mpl E-7 is a monoclonal mouse Ig M isotype, why do you suggest to use m-IgGκ BP-HRP as a secondary reagent? Is the secondary able to bind IgM as well?