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c-Kit Antibody (E-3) - Western Blotting - Image 349933
c-Kit 抗体 (E-3): sc-365504. TF-1 全细胞裂解液中 c-Kit 表达的 Western 印迹分析.
c-Kit 抗体 (E-3): sc-365504. TF-1 全细胞裂解液中 c-Kit 表达的 Western 印迹分析.

c-Kit 抗体 (E-3): sc-365504

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说明书
  • c-Kit 抗体 E-3 是小鼠单克隆 IgG1 κ，c-Kit抗体, 在32篇文献中引用，规格为200 µg/ml
  • 特定用于human起源的c-Kit的C-terminus中的氨基酸958-976之间的表位图谱
  • c-Kit 抗体 (E-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 c-Kit p145
  • 抗c-Kit抗体（E-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取c-Kit (E-3): sc-365504的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是c-Kit Antibody (E-3) for WB applications. 的首选辅助检测试剂，这些试剂现与c-Kit Antibody (E-3) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

c-Kit抗体（E-3）是一种IgG1 κ小鼠单克隆c-Kit抗体（也称为CD117抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的c-Kit蛋白。c-Kit抗体（E-3）既可作为非偶联的抗c-Kit抗体形式，也可作为多种偶联形式的抗c-Kit抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。c-Kit原癌基因是受体酪氨酸激酶家族的一员，更具体地说，它与血小板衍生生长因子受体（PDGFR）密切相关。c-Kit是HZ4-猫科肉瘤病毒转化基因（v-Kit）的正常细胞同源物，编码一种跨膜受体。c-Kit调节多种生物反应，包括趋化性、细胞增殖、凋亡和粘附。c-Kit还与小鼠W位点的产物相同，因此是肥大细胞和造血发育的组成部分。c-Kit受体的配体（KL）已被鉴定，并在小鼠steel（SI）位点编码。Kit是原癌基因c-Kit的人类同源物。Kit的突变对于各种癌症的肿瘤生长和进展都是不可或缺的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

c-Kit 抗体 (E-3) 参考文献:

  1. 从成年小鼠骨髓中纯化的 c-kit 表达造血干细胞重建胸腺。  |  de Vries, P., et al. 1992. J Exp Med. 176: 1503-9. PMID: 1281208
  2. 造血干细胞表达小鼠 c-kit 但不依赖钢因子生成的证据。  |  Ikuta, K. and Weissman, IL. 1992. Proc Natl Acad Sci U S A. 89: 1502-6. PMID: 1371359
  3. 试剂盒配体：一种在钢突变成纤维细胞中发生改变的细胞表面分子。  |  Flanagan, JG. and Leder, P. 1990. Cell. 63: 185-94. PMID: 1698555
  4. 单克隆抗体 YB5.B8 可鉴定人类 c-kit 蛋白产物。  |  Lerner, NB., et al. 1991. Blood. 77: 1876-83. PMID: 1708291
  5. 大鼠 c-kit 配体干细胞因子可诱导体内结缔组织型和粘膜肥大细胞的发育。通过解剖分布, 组织化学和蛋白酶表型进行分析。  |  Tsai, M., et al. 1991. J Exp Med. 174: 125-31. PMID: 1711559
  6. 人类原癌基因 c-kit：一种新的细胞表面受体酪氨酸激酶，用于一种未确定的配体。  |  Yarden, Y., et al. 1987. EMBO J. 6: 3341-51. PMID: 2448137
  7. 编码跨膜酪氨酸激酶受体的原癌基因 c-kit 映射到小鼠 W 基因座上。  |  Chabot, B., et al. 1988. Nature. 335: 88-9. PMID: 2457811
  8. 小鼠的显性白斑（W）基因座编码 c-kit 原癌基因。  |  Geissler, EN., et al. 1988. Cell. 55: 185-92. PMID: 2458842
  9. c-kit 蛋白，一种跨膜激酶：在组织中的鉴定和特征描述。  |  Majumder, S., et al. 1988. Mol Cell Biol. 8: 4896-903. PMID: 2463468
  10. 一种新的急性转化猫科逆转录病毒及其癌基因 v-kit 与蛋白激酶基因家族的关系。  |  Besmer, P., et al. Nature. 320: 415-21. PMID: 3007997

订购信息

产品名称产品编号规格价格数量收藏夹

c-Kit 抗体 (E-3)

sc-365504
200 µg/ml
$316.00

c-Kit (E-3): m-IgG Fc BP-HRP 套装

sc-527373
200 µg Ab; 10 µg BP
$354.00

c-Kit (E-3): m-IgG1 BP-HRP 套装

sc-532746
200 µg Ab; 20 µg BP
$354.00

c-Kit 抗体 (E-3) AC

sc-365504 AC
500 µg/ml, 25% agarose
$416.00

c-Kit 抗体 (E-3) HRP

sc-365504 HRP
200 µg/ml
$316.00

c-Kit 抗体 (E-3) FITC

sc-365504 FITC
200 µg/ml
$330.00

c-Kit 抗体 (E-3) PE

sc-365504 PE
200 µg/ml
$343.00

c-Kit 抗体 (E-3) Alexa Fluor® 488

sc-365504 AF488
200 µg/ml
$357.00

c-Kit 抗体 (E-3) Alexa Fluor® 546

sc-365504 AF546
200 µg/ml
$357.00

c-Kit 抗体 (E-3) Alexa Fluor® 594

sc-365504 AF594
200 µg/ml
$357.00

c-Kit 抗体 (E-3) Alexa Fluor® 647

sc-365504 AF647
200 µg/ml
$357.00

c-Kit 抗体 (E-3) Alexa Fluor® 680

sc-365504 AF680
200 µg/ml
$357.00

c-Kit 抗体 (E-3) Alexa Fluor® 790

sc-365504 AF790
200 µg/ml
$357.00

c-Kit (E-3) 中和勝肽

sc-365504 P
100 µg/0.5 ml
$68.00

Does this antibody work on PFA-fixed brain tissue for IHC?

Asked by: BB Barrier
Thank you for your question. c-Kit Antibody (E-3): sc-365504 is recommended for detection of c-Kit p145 of mouse, rat and human origin by WB, IP, IF and ELISA. We do not recommend using this antibody in IHC applications.
Answered by: Tech Support Europe
Date published: 2024-12-25

Which antigen retrieval technique is needed for IHC-P ? thanks

Asked by: SophiePaquet
This antibody has not yet been tested on formalin-fixed paraffin-embedded tissue sections, or for IHC(P). This antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2024-12-25

I tested sample of this antibody (1:400) with mouse thymic epithelial cells and got strong single band. However, the protein band came up around 55~60kda in 8% page gel. I am wondering if this is the right band size for c-kit ?   

Asked by: kidarime
Thank you for your question. The expected molecular weight for the c-Kit precursor is ~ 120 kDa, and mature c-Kit is ~145 kDa. We have not tested this antibody, c-Kit (E-3): sc-365504, in mouse thymic epithelial cells so we do not have any information about the abundance of the protein in these samples. However, we would not expect c-Kit to be detected at a molecular weight ~55-60 kDa. We would strongly recommend including a secondary antibody control (omit primary antibody) to determine if the lower molecular weight band you are observing is non-specific due to your secondary antibody alone. And we would also recommend increasing the primary antibody concentration and using a dilution of 1:200. And using a positive control lysate for c-Kit, such as TF-1 or TT whole cell lysate, and loading 50 µg of protein.
Answered by: Technical Support
Date published: 2017-08-24

What is the recommended positive control for c-Kit Antibody (E-3): sc-365504?

Asked by: Trav11
Thank you for your question. For c-Kit Antibody (E-3): sc-365504, we have several recommended positive controls, which include: HEL 92.1.7 Cell Lysate (sc-2270), TF-1 Cell Lysatet (sc-2412), CCRF-HSB-2 Cell Lysate (sc-2265), as well as a few others. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 2
Date published: 2017-01-18
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Rated 1 out of 5 by from WB： no stripeThe antibody was used to detect the expression of c-kit in gastric antrum of rats. Repeated operation for more than three times, using different groups of tissues, there was no band.
Date published: 2021-02-10
Rated 1 out of 5 by from Not good for WBUsed to detect c-kit in different commercially available human breast cancer cell lines, it does not work well. Produce a weak band near the expected weight and indistinguishable from different other bands in the neighboring.
Date published: 2019-09-17
Rated 1 out of 5 by from Did not work for WB analysisTried 1:500 dilution for WB analysis with human and canine bladder cancer cell lines - no signal detected
Date published: 2018-06-19
Rated 5 out of 5 by from 质量很好购买了抗体之后，特异性很好，值得推荐,希望以后有更多的优惠多多购买相关产品。如果可以多搞优惠就更好了
Date published: 2018-04-10
Rated 5 out of 5 by from I'm glad to use itWB test results are very nice, we have obtained clear, specific frequency band and background after the experiment.
Date published: 2017-10-24
Rated 5 out of 5 by from goodthis antibody was used for tracing the male germ cell in monkey ,is great when diluted 1:100.
Date published: 2017-10-10
Rated 1 out of 5 by from poor than previous antibody (sc-168G)Because sc-168 antibody is discontinued, I tested sample with the lysates from mouse thymic epithelial cell lines and got non-specific band only around 55kda. Conditions of WB ; 8% SDS-PAGE, Wet transfer (400mA/2hr), 1:400 primary antibody in 5% TBST.
Date published: 2017-08-31
Rated 5 out of 5 by from good quality antibodyThis antibody recongize the target protein very well, 1:600 dilution is strong enouth to detect the target protein .Recommend use 5% BSA as blocking buffer.
Date published: 2017-08-19
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c-Kit 抗体 (E-3) is rated 3.9 out of 5 by 21.
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