Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
C/EBP α Antibody (D-5) - Western Blotting - Image 139405
C/EBP α (D-5): sc-365318. C/EBP α 在 Caki-1 (A) 和 HeLa (B) 全细胞裂解液中表达的 Western 印迹分析.
C/EBP α Antibody (D-5) - Western Blotting - Image 139405
C/EBP α (D-5): sc-365318. C/EBP α 在 Caki-1 (A) 和 HeLa (B) 全细胞裂解液中表达的 Western 印迹分析.
C/EBP α (D-5): sc-365318. C/EBP α 在 Caki-1 (A) 和 HeLa (B) 全细胞裂解液中表达的 Western 印迹分析.

C/EBP α 抗体 (D-5): sc-365318

4.5(24)
寫評論提問

说明书
  • C/EBP alpha 抗体 D-5 是小鼠单克隆 IgM κ，C/EBP α抗体, 在64篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的C/EBP α的N-terminus的氨基酸2-25之间
  • 推荐用于 mouse, rat 和 human 来源的C/EBP α p42 WB, IP, IF 和 ELISA检测
  • 另提供Alexa Fluor® 488 偶联抗体，可用于IF、IHC(P)和FCM实验
  • 可用于ChIP的TransCruz试剂(sc-365318 X, 200 µg/0.1 ml)
  • 关于如何获取C/EBP α (D-5): sc-365318的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成C/EBP α 抗体 (D-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 C/EBP 抗体包括 C/EBP α, C/EBP β, C/EBP δ, C/EBP ε 和 C/EBP γ

C/EBP α 抗体 (D-5) 是一种小鼠单克隆 IgM 抗体，可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和酶联免疫吸附 (ELISA) 检测小鼠、大鼠和人源的 C/EBP α 蛋白。C/EBP α 抗体（D-5）有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶（HRP）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 共轭物。C/EBP alpha 蛋白是 CCAAT/增强子结合蛋白家族的成员，在调节肝脏、脂肪组织、肺部和肠道等各种组织的基因表达方面发挥着至关重要的作用。C/EBP alpha 参与多种细胞类型的分化，如肝细胞和脂肪细胞，并且对这些细胞的终极分化至关重要。C/EBP alpha 的结构具有一个基本区/亮氨酸拉链图案，可促进与 DNA 的特异性结合，从而使 C/EBP alpha 成为调节与生长和新陈代谢相关基因的转录因子。生长激素可激活 C/EBP alpha，提高 C/EBP alpha mRNA 和蛋白质水平，同时增强 DNA 结合活性，从而影响催乳素等基因的表达。C/EBP alpha 在终末分化、生长停滞细胞中的功能强调了 C/EBP alpha 在维持细胞特性和功能方面的重要性，这使得抗 C/EBP alpha 抗体 (D-5) 成为研究基因调控和细胞分化的研究人员的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

C/EBP α 抗体 (D-5) 参考文献:

  1. CCAAT/增强子结合蛋白α是催乳素基因表达的生理调节因子。  |  Jacob, KK. and Stanley, FM. 1999. Endocrinology. 140: 4542-50. PMID: 10499509
  2. 生长激素在培养的大鼠肝细胞中诱导 CCAAT/增强子结合蛋白 alpha（C/EBPalpha）。  |  Strand, P., et al. 2000. J Hepatol. 32: 618-26. PMID: 10782911
  3. 3T3-L1 细胞脂肪转化过程中三种 C/EBP 同工酶的表达调节。  |  Cao, Z., et al. 1991. Genes Dev. 5: 1538-52. PMID: 1840554
  4. 编码 CCAAT/增强子结合蛋白基因的组织特异性表达, 发育调控和遗传图谱。  |  Birkenmeier, EH., et al. 1989. Genes Dev. 3: 1146-56. PMID: 2792758
  5. 鉴定可与三种动物病毒的增强子核心元件结合的大鼠肝脏核蛋白。  |  Johnson, PF., et al. 1987. Genes Dev. 1: 133-46. PMID: 2824279
  6. 分离出编码 C/EBP 基因的重组拷贝。  |  Landschulz, WH., et al. 1988. Genes Dev. 2: 786-800. PMID: 2850264
  7. C/EBP 转录因子的 DNA 结合活性在肝细胞周期的 G1 阶段受到调控。  |  Rana, B., et al. 1995. J Biol Chem. 270: 18123-32. PMID: 7629124
  8. 体外和体内角质形成细胞分化过程中表达的转录因子 C/EBP alpha, C/EBP beta 和 CHOP (Gadd153)。  |  Maytin, EV. and Habener, JF. 1998. J Invest Dermatol. 110: 238-46. PMID: 9506442
  9. 高温诱导小鼠肝脏中的急性期蛋白基因亚群  |  Yiangou, M., et al. 1998. Biochim Biophys Acta. 1396: 191-206. PMID: 9540835

订购信息

产品名称产品编号规格价格数量收藏夹

C/EBP α 抗体 (D-5)

sc-365318
200 µg/ml
$316.00

C/EBP α 抗体 (D-5) X

sc-365318 X
200 µg/0.1 ml
$316.00

C/EBP α 抗体 (D-5) Alexa Fluor® 488

sc-365318 AF488
200 µg/ml
$357.00

C/EBP α (D-5) 中和勝肽

sc-365318 P
100 µg/0.5 ml
$68.00

Hello! I was just wondering if the C/EBP alpha antibody (D-5) was still available conjugated to FITC?

Asked by: catherine
Thank you for your question. For assistance please contact Technical service . Please call 800-457-3801or email scbt@scbt.com.
Answered by: BlakeJ
Date published: 2025-04-11

Is the epitope shared with porcine cEBPalpha? is it likely it also works with porcine cells?

Asked by: Maria
Thank you for your question. C/EBP α Antibody (D-5): sc-365318 is only recommended for detection of C/EBP α p42 of mouse, rat and human origin by WB, IP, IF and ELISA. We do not have data to support viability with porcine cell or tissue samples.
Answered by: Tech Support Europe
Date published: 2025-02-20

Can the TransCruz reagent sc-365318 X be used for applications other than ChIP or Gel Shift, such as Western Blot?

Asked by: AbPolly
Thank you for your question. Yes, the X form of the antibody can be used for all the recommended applications - just remember to dilute further since it is 10 times more concentrated than the standard form.
Answered by: Technical Support 5
Date published: 2017-01-19
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_365318, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 101ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from GreatWe use this antibody to detect C/EBPa expression in 3T3-L1 cells by western blotting. The results are great. We recommend this antibody to other researchers.
Date published: 2020-08-21
Rated 4 out of 5 by from Worked good for Western blotUsed at dilution of 1:500 in 5%BSA/TBST overnight. The band was clear but with non-specific signal.
Date published: 2018-08-09
Rated 3 out of 5 by from Pretty good, lots of non-specific bandsUsed on a western blot using 100 ug of protein from tissue. ~42 kDa band was visible and strong, just many non-specific bands.
Date published: 2018-02-21
Rated 4 out of 5 by from the antibody is goodI bought this antibody for CEBPa detection in 3T3L1 cells, bands are clear
Date published: 2018-01-30
Rated 5 out of 5 by from great antibodyWorked very well for WB and IC on mouse and rat cardiomyocytes
Date published: 2017-12-14
Rated 5 out of 5 by from greatWe use this antibody to detect C/EBP expression in the U251 cells by western blot. The results are surprising. We recommend this antibody to the researcher.
Date published: 2017-11-03
Rated 5 out of 5 by from Strong signal at expected sizeWe used this antibody at 1:200 dilution in 1% milk/TBS-T overnight. Signals shown are after 3 min of exposure to HRP/ECL reaction.
Date published: 2017-10-17
Rated 5 out of 5 by from great featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_24
  • loc_zh_CN, sid_365318, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
C/EBP α 抗体 (D-5) is rated 4.5 out of 5 by 24.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_24
  • loc_zh_CN, sid_365318, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。