Date published: 2025-9-7

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SMARCA4/Brg1 抗体 (H-10): sc-374197

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说明书
  • SMARCA4/Brg1抗体(H-10)是小鼠单克隆IgG1 κ, 在17篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Brg-1的N-terminus附近的氨基酸115-149之间
  • 抗-SMARCA4/Brg1 抗体 (H-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Brg-1; 也和以下物种反应,包括: 和 canine, bovine and porcine
  • 可用于ChIP的TransCruz试剂(sc-374197 X, 200 µg/0.1 ml)
  • 抗SMARCA4/Brg1抗体(H-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SMARCA4/Brg1 (H-10): sc-374197免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是SMARCA4/Brg1 Antibody (H-10) for WB applications. 的首选辅助检测试剂,这些试剂现与SMARCA4/Brg1 Antibody (H-10) 打包提供(请参阅下面的订购信息)。

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関連項目

Brg-1抗体(H-10)是一种IgG1 κ小鼠单克隆Brg-1抗体(也称为Brg1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Brg-1。Brg-1抗体(H-10)既可作为非偶联抗Brg-1抗体形式提供,也可作为多种偶联形式的抗Brg-1抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。SWI-SNF复合物通过以ATP依赖的方式重塑核小体结构参与转录的激活。Brm(也称为SNF2α)和Brg-1(也称为SNF2β)是哺乳动物SWI-SNF复合物的ATP酶亚基。Brm、Brg-1、Ini1(整合酶相互作用蛋白1,也称为SNF5)、BAF155(也称为SRG3)和BAF170被认为构成了SWI-SNF复合物的功能核心。向Brg-1中添加Ini1、BAF155和BAF170似乎可以增加重塑活性。其他复合物亚基被认为起调节作用。hSNF2L和hSNF2H都似乎是果蝇ISWI的同源物,ISWI是一种与Brm相关的ATP酶,存在于除SWI/SNF以外的染色质重塑复合物中,包括NURF(核小体重塑因子)。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SMARCA4/Brg1 抗体 (H-10)

sc-374197
200 µg/ml
$316.00

SMARCA4/Brg1 (H-10): m-IgG Fc BP-HRP 套装

sc-527442
200 µg Ab; 10 µg BP
$354.00

SMARCA4/Brg1 (H-10): m-IgG1 BP-HRP 套装

sc-532815
200 µg Ab; 20 µg BP
$354.00

SMARCA4/Brg1 抗体 (H-10) X

sc-374197 X
200 µg/0.1 ml
$316.00

SMARCA4/Brg1 抗体 (H-10) AC

sc-374197 AC
500 µg/ml, 25% agarose
$416.00

SMARCA4/Brg1 抗体 (H-10) HRP

sc-374197 HRP
200 µg/ml
$316.00

SMARCA4/Brg1 抗体 (H-10) FITC

sc-374197 FITC
200 µg/ml
$330.00

SMARCA4/Brg1 抗体 (H-10) PE

sc-374197 PE
200 µg/ml
$343.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 488

sc-374197 AF488
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 546

sc-374197 AF546
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 594

sc-374197 AF594
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 647

sc-374197 AF647
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 680

sc-374197 AF680
200 µg/ml
$357.00

SMARCA4/Brg1 抗体 (H-10) Alexa Fluor® 790

sc-374197 AF790
200 µg/ml
$357.00

SMARCA4/Brg1 (H-10) 中和勝肽

sc-374197 P
100 µg/0.5 ml
$68.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Brg-1 Antibody (H-10): sc-374197?

Asked by: Trav11
Thank you for your question. We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Technical Support 5
Date published: 2017-02-04
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Rated 4 out of 5 by from Brg-1 (H-10) used for western blot applicationWorks! 1/500 in 3%BSA. Fainter bands than Brg1-G7.
Date published: 2018-03-07
Rated 4 out of 5 by from ParfaitTesté en WB sur fibroblastes embryonnaires murin, il est très spécifique!
Date published: 2018-01-03
Rated 5 out of 5 by from New choice for WB of Brg-1The H-10 is a good new choice for detection of Brg-1 in Western blot. However, the G-7 clone is much more popular having been featured in over 50 publications!
Date published: 2017-01-13
Rated 5 out of 5 by from Currently the best of the Brg1 antibodies we haveBrg1 (H10) is an excellent antibody (similar to G7). We have great success using it for IPs, even with BS3-crosslinking. It is also very nice for Western Blots.
Date published: 2016-12-16
Rated 5 out of 5 by from Good WB antibodyFor 293T cell lysate, very strong signal (1:100 dilution)
Date published: 2016-12-05
Rated 5 out of 5 by from Nice WB signal achieved in KNice WB signal achieved in K-562 nuclear extract. -SCBT QC
Date published: 2015-03-30
Rated 5 out of 5 by from Very clean and specificVery clean and specific, gave beautiful bands in Western Blot.
Date published: 2015-03-25
Rated 4 out of 5 by from Beautiful band acquired in HeLa whole cell lysateBeautiful band acquired in HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-22
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SMARCA4/Brg1 抗体 (H-10) is rated 4.7 out of 5 by 9.
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