Date published: 2025-9-7
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BMPR-II Antibody (E-1) - Western Blotting - Image 356296
BMPR-II 抗体 (E-1): sc-393304. Hep G2 (A) 和 HeLa (B) 全细胞裂解液中 BMPR-II 表达的 Western 印迹分析.
BMPR-II 抗体 (E-1): sc-393304. Hep G2 (A) 和 HeLa (B) 全细胞裂解液中 BMPR-II 表达的 Western 印迹分析.

BMPR-II 抗体 (E-1): sc-393304

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说明书
  • BMPR-II 抗体 E-1 是小鼠单克隆 IgG1 κ，BMPR-II抗体, 在4篇文献中引用，规格为200 µg/ml
  • 抗原表位位于human来源BMPR-II胞外域的61-82氨基酸之间
  • BMPR-II 抗体 (E-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 BMPR-II; 也和以下物种反应，包括： 和 canine, bovine and porcine
  • 抗BMPR-II抗体（E-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取BMPR-II (E-1): sc-393304的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是BMPR-II Antibody (E-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与BMPR-II Antibody (E-1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加BMP Receptor抗体，包括BMPR-IA, BMPR-IB和BMPR-II

BMPR-II抗体（E-1）是一种IgG1 κ小鼠单克隆BMPR-II抗体（也被称为骨形态发生蛋白受体2型抗体、骨形态发生蛋白受体II型抗体、丝氨酸/苏氨酸激酶抗体、骨形态发生蛋白受体2型抗体、BMP II型受体抗体、MPR3抗体、BRK-3抗体、T-ALK抗体、PPH1抗体、II型激活素受体样激酶抗体、原发性肺动脉高压1抗体或POVD1抗体），它可以通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）和酶联免疫吸附法（ELISA）检测来自小鼠、大鼠和人类的BMPR-II蛋白。BMPR-II抗体（E-1）既可作为非偶联形式的抗BMPR-II抗体使用，也可作为多种偶联形式的抗BMPR-II抗体使用，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。转化生长因子β超家族的成员会与一对跨膜蛋白结合，这对跨膜蛋白被称为I型和II型受体，它们包含丝氨酸/苏氨酸激酶并会结合形成一个信号复合体。目前已知有两种I型受体，即BMPR-IA（也被称为SKR5、ALK-3和BRK-1）和BMPR-IB（也被称为ALK-6和SKR6），它们会与骨形态发生蛋白（BMP）-2、BMP-4和骨形成蛋白（OP）-1（也被称为BMP-7）结合。BMPR-IA和BMPR-IB都在人神经胶质瘤细胞系中表达。而II型受体BMPR-II可以有效地与OP-1和BMP-2结合，与BMP-4的结合则较弱，它在包括大脑在内的不同组织中都有广泛表达。BMP受体家族的成员被认为会以剂量依赖的方式对基因表达、细胞分化和形态发生产生不同的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

BMPR-II 抗体 (E-1) 参考文献:

  1. BMPR-II杂合小鼠患有轻度肺动脉高压，且肺血管重塑对长期缺氧的反应受损。  |  Beppu, H., et al. 2004. Am J Physiol Lung Cell Mol Physiol. 287: L1241-7. PMID: 15286002
  2. 溶酶体抑制剂氯喹可增加细胞表面BMPR-II的水平，并恢复携带BMPR-II突变内皮细胞的BMP9信号。  |  Dunmore, BJ., et al. 2013. Hum Mol Genet. 22: 3667-79. PMID: 23669347
  3. siRNA靶向BMPR-II对人类肝癌细胞的生物学活性的影响及其机制。  |  Zeng, P., et al. 2014. Cancer Cell Int. 14: 55. PMID: 25002834
  4. BMPR-II突变通过超炎症反应促进肺动脉高压。  |  Soon, E., et al. 2015. Clin Med (Lond). 15 Suppl 3: s15. PMID: 26026008
  5. 成釉细胞瘤和类腺瘤牙源性肿瘤中BMP-2和BMP-4及其受体BMPR-IA和BMPR-II的免疫表达。  |  Nascimento, MA., et al. 2017. Arch Oral Biol. 73: 223-229. PMID: 27780042
  6. 肺动脉高压发病机制中的TGFβ和BMPRII信号通路。  |  Tielemans, B., et al. 2019. Drug Discov Today. 24: 703-716. PMID: 30529762
  7. 骨形态发生蛋白人类 II 型受体的克隆和特征描述。  |  Rosenzweig, BL., et al. 1995. Proc Natl Acad Sci U S A. 92: 7632-6. PMID: 7644468
  8. 骨形态发生蛋白（BMPs）的人类 II 型受体：将双激酶受体模型扩展到 BMPs。  |  Liu, F., et al. 1995. Mol Cell Biol. 15: 3479-86. PMID: 7791754
  9. 鉴定成骨蛋白-1 和骨形态发生蛋白-4 的 I 型受体。  |  ten Dijke, P., et al. 1994. J Biol Chem. 269: 16985-8. PMID: 8006002
  10. 骨形态发生蛋白 IB 型受体在恶性胶质瘤肿瘤中逐渐表达。  |  Yamada, N., et al. 1996. Br J Cancer. 73: 624-9. PMID: 8605097
  11. 发育中和成年大鼠大脑中丝氨酸/苏氨酸激酶受体（包括骨形态发生因子 II 型受体）的表达。  |  Söderström, S., et al. 1996. Cell Tissue Res. 286: 269-79. PMID: 8854897
  12. 骨形态发生蛋白受体和激活素受体在后纵韧带骨化患者的骨化韧带组织中高度表达。  |  Yonemori, K., et al. 1997. Am J Pathol. 150: 1335-47. PMID: 9094990

订购信息

产品名称产品编号规格价格数量收藏夹

BMPR-II 抗体 (E-1)

sc-393304
200 µg/ml
$316.00

BMPR-II (E-1): m-IgGκ BP-HRP 套装

sc-523895
200 µg Ab, 40 µg BP
$354.00

BMPR-II (E-1): m-IgG1 BP-HRP 套装

sc-533619
200 µg Ab; 20 µg BP
$354.00

BMPR-II 抗体 (E-1) AC

sc-393304 AC
500 µg/ml, 25% agarose
$416.00

BMPR-II 抗体 (E-1) HRP

sc-393304 HRP
200 µg/ml
$316.00

BMPR-II 抗体 (E-1) FITC

sc-393304 FITC
200 µg/ml
$330.00

BMPR-II 抗体 (E-1) PE

sc-393304 PE
200 µg/ml
$343.00

BMPR-II 抗体 (E-1) Alexa Fluor® 488

sc-393304 AF488
200 µg/ml
$357.00

BMPR-II 抗体 (E-1) Alexa Fluor® 546

sc-393304 AF546
200 µg/ml
$357.00

BMPR-II 抗体 (E-1) Alexa Fluor® 594

sc-393304 AF594
200 µg/ml
$357.00

BMPR-II 抗体 (E-1) Alexa Fluor® 647

sc-393304 AF647
200 µg/ml
$357.00

BMPR-II 抗体 (E-1) Alexa Fluor® 680

sc-393304 AF680
200 µg/ml
$357.00

BMPR-II 抗体 (E-1) Alexa Fluor® 790

sc-393304 AF790
200 µg/ml
$357.00

BMPR-II (E-1) 中和勝肽

sc-393304 P
100 µg/0.5 ml
$68.00

Do you have data demonstrating that BMPR-II Antibody (E-1) Alexa Fluor® 647 detects BMPR-II on the surface of mouse cells?

Asked by: LisaD
Thank you for your question. We do not have any images for AF647 but we do have a warranty for one year from date of purchase .
Answered by: BlakeJ
Date published: 2023-05-10

Is the BMPR-II Antibody (E-1) Alexa Fluor® 680 suitable for flow cytometry and if so, do you have data demonstrating its use?

Asked by: Frankie Wignall
The conjugated primary antibody is recommended and warrantied for flow cytometry (FCM), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2018-12-05

What application is the blocking peptide sc-393304 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works great for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:500 for overnight @ 4oC.
Date published: 2024-12-10
Rated 1 out of 5 by from Did not work for BMMCs IFTried 1:100 dilution using mouse BMMCs for IF and no signal detected
Date published: 2018-06-21
Rated 4 out of 5 by from sc-393304 REVIEWWe tried this antibody by western blot for several times, using samples of bovine pulmonary arteries. Our data suggested the first antibody dilution was 1:100 and 5% BSA was better than 5% milk as the dilution buffer. We got double bands above 95KDa when we used 5% milk as dilution buffer, but single band with 5% BSA. The bands were easier to get and clearer when using 5% BSA as dilution buffer.
Date published: 2017-07-11
Rated 5 out of 5 by from Good antibodyHUVEC lysate 30ug Great signal in Western Blotting on HUVEC using it at a 1:1000 dilution.
Date published: 2017-07-03
Rated 5 out of 5 by from Strong WB monoclonalGreat signal in Western Blotting on HeLa cells using it at a 1:200 dilution.
Date published: 2017-01-18
Rated 5 out of 5 by from Highly potent and specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~115 kDa target protein in HeLa/Jurkat cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Good specificity and signal strength in HepGood specificity and signal strength in Hep G2 and TE671 whole cell lysates. -SCBT QC
Date published: 2015-02-11
Rated 5 out of 5 by from Produced positive Western blot data of BMPRProduced positive Western blot data of BMPR-II expression in IMR-32 and A-431 whole cell lysates. -SCBT QC
Date published: 2013-11-15
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BMPR-II 抗体 (E-1) is rated 4.4 out of 5 by 8.
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