Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BMPR-IA CRISPR/Cas9 KO Plasmid (h2): sc-400581-KO-2
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BMPR-IA CRISPR/Cas9 Knockout (KO) Plasmid (h2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BMPR-IA genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BMPR-IA Antibody (7K7): sc-134285
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BMPR-IA CRISPR/Cas9 KO Plasmid (h2) | sc-400581-KO-2 | 20 µg | $397.00 |
Overview
BMPR1A encodes bone morphogenetic protein receptor type IA (BMPR-IA), a serine/threonine kinase receptor that binds BMP ligands and initiates canonical SMAD1/5/9 signaling as well as non-canonical MAPK pathways. Through these cascades, BMPR-IA regulates cell fate specification, proliferation, differentiation, and tissue morphogenesis, with prominent roles in osteogenic programs and epithelial homeostasis. Altered BMPR1A signaling is implicated in developmental abnormalities and cancer-relevant processes via dysregulated BMP/TGF-β pathway activity, including effects on stem cell dynamics and growth control. BMPR1A is therefore widely studied in contexts such as lineage commitment, organoid biology, and signal transduction crosstalk.
BMPR-IA CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the BMPR1A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BMPR1A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BMPR1A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BMPR-IA protein expression.
This CRISPR knockout system enables efficient generation of BMPR1A-deficient cell models for investigation of BMPR-IA signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BMPR1A exon(s) critical for BMPR-IA function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BMPR1A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BMPR-IA CRISPR/Cas9 KO Plasmid (h) and BMPR-IA CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BMPR1A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BMPR-IA HDR Plasmid (h) and BMPR-IA HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BMPR1A homology arms to support homology-directed repair at defined BMPR1A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.