Date published: 2025-9-11

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Biotin 抗体 (BK-1/39): sc-53179

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说明书
  • Biotin 抗体 BK-1/39 是小鼠单克隆 IgG1 κ,Biotin抗体, 在7篇文献中引用,规格为200 µg/ml
  • 与Biotin结合,与匙孔血蓝蛋白结合
  • Biotin 抗体 (BK-1/39) 推荐用于 WB, IP 和 IF,检测 Biotin ; 允许形成抗体-Biotin复合物,从而提高检测系统的灵敏度
  • 抗Biotin抗体(BK-1/39)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Biotin (BK-1/39): sc-53179免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Biotin Antibody (BK-1/39) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Biotin Antibody (BK-1/39) 打包提供(请参阅下面的订购信息)。

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関連項目

生物素抗体(BK-1/39)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测多种生物素蛋白。抗生物素抗体(BK-1/39)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®选项。生物素,又称维生素B7、维生素H或辅酶R,在细胞代谢中发挥着至关重要的作用,特别是在合成脂肪酸和氨基酸所必需的羧化反应中,以及在糖异生中。生物素的结构特征是四氢噻吩环与脲基环融合,这使得这种蛋白质作为催化关键代谢途径的羧化酶的辅助因子至关重要。虽然某些微生物和植物可以合成生物素,但饮食来源也能提供这种必需蛋白质,缺乏这种蛋白质虽然罕见,但会导致皮炎、脱发和神经系统症状。生物素在自然界中广泛存在,这凸显了这种蛋白质在维持各种生物的新陈代谢健康方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Biotin 抗体 (BK-1/39) 参考文献:

  1. 抗生物素蛋白-生物素技术简介。  |  Wilchek, M. and Bayer, EA. 1990. Methods Enzymol. 184: 5-13. PMID: 2201884
  2. 诊断测试中的生物素干扰。  |  Chun, KY. 2017. Clin Chem. 63: 619-620. PMID: 28130485
  3. 生物素酶的调节。  |  Dakshinamurti, K. and Chauhan, J. 1988. Annu Rev Nutr. 8: 211-33. PMID: 2904822
  4. 生物素功能化的靶向聚二乙炔胶束。  |  Doerflinger, A., et al. 2018. Chem Commun (Camb). 54: 3613-3616. PMID: 29577125
  5. 基于生物素-链霉亲和素化学的免疫测定中的生物素干扰:一种新兴威胁。  |  Luong, JHT., et al. 2019. Biotechnol Adv. 37: 634-641. PMID: 30872068
  6. 妊娠剧吐中的生物素缺乏。  |  Onder, AB., et al. 2019. J Obstet Gynaecol. 39: 1160-1163. PMID: 31335232
  7. 严重肥胖症中肠道微生物生物素代谢和宿主生物素状态的损害:生物素和益生元补充剂对改善代谢的影响。  |  Belda, E., et al. 2022. Gut. 71: 2463-2480. PMID: 35017197
  8. 重新审视生物素反应性疾病患儿生物素的给药。  |  Wolf, B. 2022. Mol Genet Metab. 137: 225-227. PMID: 35843775
  9. 免疫测定设计和生物素干扰。  |  Dasgupta, A. 2022. Adv Clin Chem. 109: 165-183. PMID: 35953126
  10. 分枝杆菌生物素合酶需要辅助蛋白将脱硫生物素转化为生物素。  |  Qu, D., et al. 2024. Nat Commun. 15: 4161. PMID: 38755122

订购信息

产品名称产品编号规格价格数量收藏夹

Biotin 抗体 (BK-1/39)

sc-53179
200 µg/ml
$316.00

Biotin (BK-1/39): m-IgG Fc BP-HRP 套装

sc-528558
200 µg Ab; 10 µg BP
$354.00

Biotin (BK-1/39): m-IgGκ BP-HRP 套装

sc-520958
200 µg Ab, 40 µg BP
$354.00

Biotin (BK-1/39): m-IgG1 BP-HRP 套装

sc-542993
200 µg Ab; 20 µg BP
$354.00

Biotin 抗体 (BK-1/39) AC

sc-53179 AC
500 µg/ml, 25% agarose
$416.00

Biotin 抗体 (BK-1/39) HRP

sc-53179 HRP
200 µg/ml
$316.00

Biotin 抗体 (BK-1/39) FITC

sc-53179 FITC
200 µg/ml
$330.00

Biotin 抗体 (BK-1/39) PE

sc-53179 PE
200 µg/ml
$343.00

Biotin 抗体 (BK-1/39) Alexa Fluor® 488

sc-53179 AF488
200 µg/ml
$357.00

Biotin 抗体 (BK-1/39) Alexa Fluor® 546

sc-53179 AF546
200 µg/ml
$357.00

Biotin 抗体 (BK-1/39) Alexa Fluor® 594

sc-53179 AF594
200 µg/ml
$357.00

Biotin 抗体 (BK-1/39) Alexa Fluor® 647

sc-53179 AF647
200 µg/ml
$357.00

Biotin 抗体 (BK-1/39) Alexa Fluor® 680

sc-53179 AF680
200 µg/ml
$357.00

Biotin 抗体 (BK-1/39) Alexa Fluor® 790

sc-53179 AF790
200 µg/ml
$357.00

I would like to receive a 10 µg GRATIS Sample from the Biotin (BK-1/39): sc-53179 antibody. Would like to test if it works for non-radioactive EMSA assay for detection of Biotin labelled oligos.

Asked by: Tzvete
Thank you for your question. Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service 1
Date published: 2023-09-29

Can I use Biotin (BK-1/39): sc-53179 monoclonal antibody for ChIP?

Asked by: cjMara
We have not tested this Biotin (BK-1/39): sc-53179 mouse monoclonal antibody for Chromatin Immunoprecipitation (ChIP). However, there is a recent publication validating the use of this antibody for this research application. Please refer to PMID: 27639690 (also available on our website, listed under the product citations for this antibody).
Answered by: Technical Support 10
Date published: 2017-01-20
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Rated 3 out of 5 by from it's working.. butThe biotin antibody seems to work but too much background and I need to use quite high amount of protein to detect the biotinylated target protein.
Date published: 2017-10-31
Rated 5 out of 5 by from Works greatWorks great for WB at 1:1000 dilution in HeLa and 293T cells.
Date published: 2017-09-06
Rated 5 out of 5 by from Specific WB dataGreat product for western blot applications; strong, specific signal
Date published: 2017-02-05
Rated 5 out of 5 by from Nice antibody for Biotin!This is a great choice if you're looking for an antibody for biotin detection. Strong and specific.
Date published: 2017-01-10
Rated 4 out of 5 by from Bands seen in WB using Biotin conjugatedBands seen in WB using Biotin conjugated normal goat IgG. -SCBT QC
Date published: 2014-06-11
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Biotin 抗体 (BK-1/39) is rated 4.4 out of 5 by 5.
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