Date published: 2025-9-6

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β-dystroglycan 抗体 (4F7): sc-33702

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说明书
  • beta-dystroglycan 抗体 4F7 是小鼠单克隆 IgG2b κ,β-dystroglycan抗体, 在20篇文献中引用,规格为200 µg/ml
  • 与human的C-terminus β-dystroglycan
  • beta-dystroglycan 抗体 (4F7) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 β-dystroglycan
  • 抗beta-dystroglycan抗体(4F7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取β-dystroglycan (4F7): sc-33702免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 β-dystroglycan 抗体 (4F7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与β-dystroglycan 抗体 (4F7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

β-dystroglycan 抗体 (4F7) 是一种小鼠单克隆 IgG2b kappa 轻链抗体,可通过蛋白质印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和免疫组织化学检测小鼠、大鼠和人类来源的 β-dystroglycan。β-dystroglycan抗体(4F7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。β-肌动蛋白作为细胞表面受体,在多种细胞外基质成分(如层粘连蛋白、神经节苷脂和珍珠蛋白)的细胞表面受体中发挥着至关重要的作用,这些成分对于早期发育中基底膜的形成和细胞表面层粘连蛋白的正常组织至关重要。这种功能可以维持组织完整性并促进细胞信号传导。β-dystroglycan与α-dystroglycan形成复合物,将α-dystroglycan锚定在细胞膜上,并参与更大的多分子复合物,包括肌细胞膜上的dystrophin和神经肌肉连接处的utrophin。这些相互作用对于聚集烟碱型乙酰胆碱受体至关重要,而烟碱型乙酰胆碱受体是有效的神经肌肉传递所必需的。抗-β-肌萎缩蛋白抗体(4F7)是研究肌肉生物学和相关病理学的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β-dystroglycan 抗体 (4F7) 参考文献:

  1. 缺乏肌营养不良糖的奇异小鼠会患上肌肉营养不良症,肌神经突触也会受到破坏。  |  Côté, PD., et al. 1999. Nat Genet. 23: 338-42. PMID: 10610181
  2. β-肌收缩蛋白-生长因子受体 2 (Grb2) 相互作用的特征。  |  Russo, K., et al. 2000. Biochem Biophys Res Commun. 274: 93-8. PMID: 10903901
  3. 突触后膜上肌营养不良蛋白复合物成分的不同靶向性。  |  Marchand, S., et al. 2001. Eur J Neurosci. 13: 221-9. PMID: 11168526
  4. dystroglycan, β1整合素和perlecan在细胞表面层粘蛋白组织中的不同作用。  |  Henry, MD., et al. 2001. J Cell Sci. 114: 1137-44. PMID: 11228157
  5. 背根神经节卫星细胞中肌冻蛋白复合物的表达。  |  Masaki, T., et al. 2001. Acta Neuropathol. 101: 174-8. PMID: 11271373
  6. 合成携带新型甘露糖丝氨酸/苏氨酸连接的α-肌营养不良衍生糖基氨基酸。  |  Seifert, J., et al. 2000. Glycoconj J. 17: 407-23. PMID: 11294507
  7. 在神经元活动增强的情况下,β-肌营养不良蛋白是 MMP-9 的靶标。  |  Michaluk, P., et al. 2007. J Biol Chem. 282: 16036-41. PMID: 17426029
  8. 从质膜到细胞核的β-缓激肽逆向运输。  |  Gracida-Jiménez, V., et al. 2017. Sci Rep. 7: 9906. PMID: 28852008
  9. 在发育中的大鼠大脑中,β-缓激肽的出现先于胶质血管端足的形成。  |  Kálmán, M., et al. 2018. Eur J Histochem. 62: 2908. PMID: 29943956
  10. 前梯度 2 参与β-缓激肽的转录后调控。  |  Lee, E. and Lee, DH. 2021. Anim Cells Syst (Seoul). 25: 19-27. PMID: 33717413
  11. MiR-132通过基质金属蛋白酶-9在原发性神经元中的上调下调高血糖诱导的β-缓激肽降解。  |  Dou, Y., et al. 2021. J Cell Mol Med. 25: 7783-7795. PMID: 34160889
  12. α-肌收缩蛋白-β-肌收缩蛋白复合物。膜组织以及与 Agrin 受体的关系。  |  Deyst, KA., et al. 1995. J Biol Chem. 270: 25956-9. PMID: 7592785

订购信息

产品名称产品编号规格价格数量收藏夹

β-dystroglycan 抗体 (4F7)

sc-33702
200 µg/ml
$316.00

β-dystroglycan (4F7): m-IgG Fc BP-HRP 套装

sc-528488
200 µg Ab; 10 µg BP
$354.00

β-dystroglycan (4F7): m-IgGκ BP-HRP 套装

sc-520879
200 µg Ab, 40 µg BP
$354.00

β-dystroglycan (4F7): m-IgG2b BP-HRP 套装

sc-550043
200 µg Ab; 10 µg BP
$354.00

β-dystroglycan 抗体 (4F7) AC

sc-33702 AC
500 µg/ml, 25% agarose
$416.00

β-dystroglycan 抗体 (4F7) HRP

sc-33702 HRP
200 µg/ml
$316.00

β-dystroglycan 抗体 (4F7) FITC

sc-33702 FITC
200 µg/ml
$330.00

β-dystroglycan 抗体 (4F7) PE

sc-33702 PE
200 µg/ml
$343.00

β-dystroglycan 抗体 (4F7) Alexa Fluor® 488

sc-33702 AF488
200 µg/ml
$357.00

β-dystroglycan 抗体 (4F7) Alexa Fluor® 546

sc-33702 AF546
200 µg/ml
$357.00

β-dystroglycan 抗体 (4F7) Alexa Fluor® 594

sc-33702 AF594
200 µg/ml
$357.00

β-dystroglycan 抗体 (4F7) Alexa Fluor® 647

sc-33702 AF647
200 µg/ml
$357.00

β-dystroglycan 抗体 (4F7) Alexa Fluor® 680

sc-33702 AF680
200 µg/ml
$357.00

β-dystroglycan 抗体 (4F7) Alexa Fluor® 790

sc-33702 AF790
200 µg/ml
$357.00

What is the sequence of the antigen aginst which this monoclonal was raised?

Asked by: ProfSteve
Thank you for your question. This antibody is made against a sequence mapping at the c-terminus of the human β-dystroglycan protein (accession#Q14118). For more detailed information, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-01-10

How can β-dystroglycan (4F7): sc-33702 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenniferc
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. β-dystroglycan (4F7): sc-33702 monoclonal antibody is currently available conjugated to phycoerythrin (sc-33702 PE), fluorescein (sc-33702 FITC), Alexa Fluor® 488 (sc-33702 AF488) or Alexa Fluor® 647 (sc-33702 AF647)
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 4 out of 5 by from Buon funzionamentoHo testato questo anticorpo su lisato totale di ippocampo murino e sono molto soddisfatta della resa ottenuta
Date published: 2024-06-14
Rated 5 out of 5 by from Great antibody !!Outstanding western blot, Also very nice IHC and IF staining data
Date published: 2017-01-13
Rated 5 out of 5 by from Works well for immunfluorescenceThe beta-Dystroglycan Antibody (4F7) shows strong, localized staining pattern in immunofluorescence applications with several publication citations.
Date published: 2016-12-21
Rated 4 out of 5 by from Published data for IP and ICC in rat hippocampalPublished data for IP and ICC in rat hippocampal neuron cultures, COS-7. -SCBT Publication Review
Date published: 2015-06-30
Rated 4 out of 5 by from Strong positive band obtained in WB in ratStrong positive band obtained in WB in rat skeletal muscle tissue extract and SK-BR-3 whole cell lysate. -SCBT QC
Date published: 2015-05-08
Rated 4 out of 5 by from Produced clear immunoperoxidase membraneProduced clear immunoperoxidase membrane staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-03-20
Rated 4 out of 5 by from Published IHC(P) data for use in human PDAC cellsPublished IHC(P) data for use in human PDAC cells. -SCBT Publication Review
Date published: 2015-01-27
Rated 4 out of 5 by from Clear band of interest detected in WB inClear band of interest detected in WB in C6 and L6 whole cell lysates. -SCBT QC
Date published: 2015-01-13
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β-dystroglycan 抗体 (4F7) is rated 4.3 out of 5 by 8.
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