Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Bcl10 Antibody (331.3) - Western Blotting - Image 174
Bcl10 抗体 (331. 3): sc-5273. KNRK (A), WR19L (B), HuT 78 (C), MOLT-4 (D), U-937 (E) 和 NAMALWA (F) 全细胞裂解液中 Bcl10 表达的 Western 印迹分析.
Bcl10 抗体 (331. 3): sc-5273. KNRK (A), WR19L (B), HuT 78 (C), MOLT-4 (D), U-937 (E) 和 NAMALWA (F) 全细胞裂解液中 Bcl10 表达的 Western 印迹分析.

Bcl10 抗体 (331.3): sc-5273

4.7(14)
寫評論提問

说明书
  • Bcl10 抗体 331.3 是小鼠单克隆 IgG1 κ，Bcl10抗体, 在98篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Bcl10的氨基酸168-233
  • Bcl10 抗体 (331.3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Bcl10
  • 抗Bcl10抗体（331.3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Bcl10 (331.3): sc-5273的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Bcl10 抗体 (331.3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Bcl10 抗体 (331.3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

Bcl10抗体（331.3）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学和酶联免疫吸附等方法检测小鼠、大鼠和人类来源的Bcl10。Bcl10 (331.3) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物。Bcl10，也称为CIPER、c-CARMEN和mE10，在免疫信号传导中起着至关重要的作用，最初被确定为MALT B细胞淋巴瘤和其他肿瘤类型中截短或突变的基因。Bcl10的结构特征是N端有半胱天冬酶招募结构域（CARD），该结构域通过NIK依赖机制激活NFκB信号通路，对激活NFκB信号通路至关重要。这种激活非常重要，因为NFκB是免疫反应和细胞存活的关键调节因子，使Bcl10成为调控细胞凋亡和炎症的重要成分。此外，Bcl10还与半胱天冬酶-9加工成活性二聚体有关，突出了其在细胞凋亡信号传导中的重要性。虽然Bcl10在许多人类肿瘤和淋巴瘤中并未发生突变，但过表达可通过CARD介导的寡聚化诱导细胞凋亡，这凸显了其在癌症研究中作为治疗靶标的潜力。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Bcl10 抗体 (331.3) 参考文献:

  1. BCL10 在正常和肿瘤性淋巴组织中的表达。在 MALT 淋巴瘤中的核定位。  |  Ye, H., et al. 2000. Am J Pathol. 157: 1147-54. PMID: 11021819
  2. Bcl10 是抗原受体诱导的 NF-kappaB 激活和神经管闭合的正向调节因子。  |  Ruland, J., et al. 2001. Cell. 104: 33-42. PMID: 11163238
  3. Bcl10和MALT1是麦芽淋巴瘤染色体易位的独立靶点，它们在新型NF-kappa B信号通路中相互合作。  |  Lucas, PC., et al. 2001. J Biol Chem. 276: 19012-9. PMID: 11262391
  4. Bcl10 与 TRAF2 和 cIAPs 的相互结合调节着细胞凋亡信号的传递。  |  Yui, D., et al. 2001. Oncogene. 20: 4317-23. PMID: 11466612
  5. Bcl10。  |  Thome, M. and Tschopp, J. 2002. Curr Biol. 12: R45. PMID: 11818074
  6. Bcl10 通过泛素化 NEMO 激活 NF-kappaB 通路。  |  Zhou, H., et al. 2004. Nature. 427: 167-71. PMID: 14695475
  7. Bcl10 在 B 细胞发育和 LPS 反应中的新作用  |  Fischer, KD., et al. 2004. Trends Immunol. 25: 113-6. PMID: 15049289
  8. 由 T 细胞活化诱导的 Bcl10 降解可对 NF-kappa B 信号进行负向调节。  |  Scharschmidt, E., et al. 2004. Mol Cell Biol. 24: 3860-73. PMID: 15082780
  9. BCL10通过与Pellino2的相互作用介导脂多糖/类托尔受体-4信号传导。  |  Liu, Y., et al. 2004. J Biol Chem. 279: 37436-44. PMID: 15213237
  10. 伴有t(14;18)(q32;q21)/IGH-MALT1的MALT淋巴瘤的特点是细胞质中MALT1和BCL10的强表达。  |  Ye, H., et al. 2005. J Pathol. 205: 293-301. PMID: 15682443
  11. Bcl10 可促进抗原刺激的 B 淋巴细胞存活。  |  Tian, MT., et al. 2005. Blood. 106: 2105-12. PMID: 15878976
  12. DNA损伤后细胞存活中的BCL10。  |  Luo, Y., et al. 2019. Clin Chim Acta. 495: 301-308. PMID: 31047877

订购信息

产品名称产品编号规格价格数量收藏夹

Bcl10 抗体 (331.3)

sc-5273
200 µg/ml
$316.00

Bcl10 (331.3): m-IgG Fc BP-HRP 套装

sc-526476
200 µg Ab; 10 µg BP
$354.00

Bcl10 (331.3): m-IgG1 BP-HRP 套装

sc-531849
200 µg Ab; 20 µg BP
$354.00

Bcl10 抗体 (331.3) AC

sc-5273 AC
500 µg/ml, 25% agarose
$416.00

Bcl10 抗体 (331.3) HRP

sc-5273 HRP
200 µg/ml
$316.00

Bcl10 抗体 (331.3) FITC

sc-5273 FITC
200 µg/ml
$330.00

Bcl10 抗体 (331.3) PE

sc-5273 PE
200 µg/ml
$343.00

Bcl10 抗体 (331.3) Alexa Fluor® 488

sc-5273 AF488
200 µg/ml
$357.00

Bcl10 抗体 (331.3) Alexa Fluor® 546

sc-5273 AF546
200 µg/ml
$357.00

Bcl10 抗体 (331.3) Alexa Fluor® 594

sc-5273 AF594
200 µg/ml
$357.00

Bcl10 抗体 (331.3) Alexa Fluor® 647

sc-5273 AF647
200 µg/ml
$357.00

Bcl10 抗体 (331.3) Alexa Fluor® 680

sc-5273 AF680
200 µg/ml
$357.00

Bcl10 抗体 (331.3) Alexa Fluor® 790

sc-5273 AF790
200 µg/ml
$357.00

quel témoin positif peut on utiliser? ltissu lymphoide de l'appendice, de l'amygdale ou ganglion lymphatique? merci de votre aide

Asked by: geneviève
Merci pour votre question. Sur la fiche technique de cet anticorps, vous trouverez des exemples de lysats cellulaires recommandés en contrôles positifs pour des expériences de Western Blot. Sur cette page web, nous listons également des publications pour cet anticorps pour d'autres applications, telles que l'immunohistochimie, dans lesquelles vous trouverez des informations complémentaires sur les tissus utilisés avec cet anticorps.
Answered by: Technical Support Europe
Date published: 2019-03-05

Does this vial contain only the light chain (and no heavy chain)? I am using this for immunoprecipitation and would like to know if I would see the ~50kDa heavy chain band on the western blot.

Asked by: Chris170124
Thank you for your question. Bcl10 Antibody (331.3): sc-5273 is a mouse monoclonal primary antibody that contains the entire immunoglobulin molecule (heavy and light chains). If you use this antibody for immunoprecipitation of Bcl10, you would expect to see the heavy (~50 kDa) and light (~25 kDa) chains of the IgG if you are using a conventional anti-mouse IgG secondary antibody for detection in your Western blot. Because Bcl10 has an expected molecular weight of ~ 33 kDa, which is similar to the size of the IgG light chain, you may want to avoid using a secondary antibody in your Western blot. As an alternative, we would recommend using your primary antibody directly conjugated to HRP (sc-5273 HRP) if you are using a chemiluminescent detection system. The isotype of this primary antibody is IgG1, and the type of light chain is kappa. This information is provided on our website to help you select the appropriate secondary detection reagent, one of our recommended mouse IgG binding proteins, specific for kappa light chain.
Answered by: Technical Support
Date published: 2017-01-24

I would like to use the Bcl10 Antibody (331.3) for IHC staining of mouse tissue. Is this possible?

Asked by: Trav11
Thank you for your question. Yes, this is absolutely possible. In this case, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, Bcl10 Antibody (331.3): sc-5273 is available directly conjugated to HRP (sc-5273 HRP), phycoerythrin (sc-5273 PE), fluoroscein (sc-5273 FITC), Alexa Fluor® 488 (sc-5273 AF488) or Alexa Fluor® 647 (sc-5273 AF647). If you have any further questions, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-29
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_5273, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from 染色性が素晴らしい。膵臓の腺房細胞癌症例(FFPE切片)で免疫染色したところ、腫瘍細胞特異的にすばらしい染色性を示した。
Date published: 2020-08-19
Rated 5 out of 5 by from Nice antibodyVery clean & straightforward staining. Easy to use. Perfect!
Date published: 2019-11-06
Rated 4 out of 5 by from good functionthis antibody is good，my samples were the primary Kupffers from rat.
Date published: 2017-09-18
Rated 4 out of 5 by from a few non-specifc bandI've used the antibody a few times according to the protocol ,it worked well for me. 20 and 30 ug protein gave a strong signal after ECL. but also a constant non-specific band appeared.
Date published: 2017-07-22
Rated 5 out of 5 by from reliable antibodyExcellent signal obtained in WB. Specificity shown by sensitivity to Bcl10 knockdown by siRNA.
Date published: 2017-01-06
Rated 5 out of 5 by from Works wellThis antibody works well in Western Blotting for detecting Bcl10 of human origin. The dilution in my experiment is 1:500. This dilution gives gives good signal.
Date published: 2016-11-23
Rated 5 out of 5 by from This antibody gave strong band in 3 differentThis antibody gave strong band in 3 different human cell lines, works well in WB.
Date published: 2015-09-28
Rated 5 out of 5 by from Great signal in multiple cell lines such as CTLLGreat signal in multiple cell lines such as CTLL-2, MM-142, BYDP, RAW 264.7, WEHI-231 and IB4 whole cell lysates. -SCBT QC
Date published: 2015-04-28
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_13
  • loc_zh_CN, sid_5273, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 17ms
  • REVIEWS, PRODUCT
Bcl10 抗体 (331.3) is rated 4.7 out of 5 by 14.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_1, tr_13
  • loc_zh_CN, sid_5273, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 101ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。