Bcl-2 Antibody (SPM117): sc-56018

Bcl-2 Antibody (SPM117) is a mouse monoclonal IgG1 antibody that detects Bcl-2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Bcl-2 plays a crucial role in regulating apoptosis, which is vital for maintaining tissue homeostasis and protecting against diseases, including cancer. This anti-apoptotic protein is primarily located in the outer mitochondrial membrane, where Bcl-2 functions to inhibit the release of pro-apoptotic factors, thereby preventing cell death. The localization of Bcl-2 is significant because direct modulation of mitochondrial integrity influences cellular fate in response to stress signals. By stabilizing the mitochondrial membrane, Bcl-2 can effectively block the activation of apoptotic pathways, such as the cytochrome c/Apaf-1 cascade, which is essential for the initiation of programmed cell death. Dysregulation of Bcl-2 expression, often due to mutations or overexpression, can lead to the evasion of apoptosis, contributing to the development and progression of various cancers. Anti-Bcl-2 antibody (SPM117) is an invaluable tool for researchers studying the mechanisms of apoptosis and its implications in cancer biology.

The preferred Bcl-2 antibody is Bcl-2 Antibody (C-2): sc-7382.