Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BABAM1 CRISPR/Cas9 KO Plasmid (h): sc-409906
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BABAM1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BABAM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BABAM1 Antibody (H-10): sc-398570
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BABAM1 CRISPR/Cas9 KO Plasmid (h) | sc-409906 | 20 µg | $397.00 |
Overview
BABAM1 (BABAM1) is a core component of the BRCA1-A complex that recognizes ubiquitinated chromatin at sites of DNA double-strand breaks and helps coordinate the DNA damage response. Through interactions with BRCA1 and associated factors, BABAM1 supports genome stability by modulating damage signaling and influencing pathway choice during repair, including processes linked to homologous recombination regulation. Its activity connects to cell-cycle checkpoint control and replication stress responses, making it relevant to studies of chromatin-dependent repair coordination. Dysregulation of BRCA1-A complex function has been associated with altered DNA repair capacity and genomic instability phenotypes observed across multiple cancer contexts.
BABAM1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BABAM1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BABAM1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BABAM1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BABAM1 protein expression.
This CRISPR knockout system enables efficient generation of BABAM1-deficient cell models for investigation of BABAM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BABAM1 exon(s) critical for BABAM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BABAM1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BABAM1 CRISPR/Cas9 KO Plasmid (h) and BABAM1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BABAM1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BABAM1 HDR Plasmid (h) and BABAM1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BABAM1 homology arms to support homology-directed repair at defined BABAM1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.