Date published: 2026-6-3

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B23/Nucleophosmin Antibody (7H10B9): sc-65943

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Datasheets
  • B23/Nucleophosmin Antibody (7H10B9) is a mouse monoclonal IgG1 provided at 100 µg/ml
  • raised against B23 recombinant protein of human origin
  • recommended for detection of B23 of human origin by WB, IP, IF and IHC(P)
  • See B23/Nucleophosmin (E-3): sc-271737 for B23/Nucleophosmin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for B23/Nucleophosmin Antibody (7H10B9). This work is in progress.
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    B23 Antibody (7H10B9) is a mouse monoclonal IgG1 antibody that detects B23 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. B23, also known as nucleophosmin, plays a crucial role in ribosomal assembly and the transport of ribosomal RNA, making B23 essential for protein synthesis and cellular function. B23 is predominantly located in the nucleolus, where B23 is involved in the maturation of ribosomal subunits, and proper localization is vital for maintaining efficient ribosome biogenesis. Additionally, B23 undergoes significant post-translational modifications, particularly phosphorylation by Cdc2 kinase during mitosis, which can influence B23′s activity and interactions with other cellular proteins. Anti-B23 antibody (7H10B9) serves as an invaluable tool for researchers studying ribosome assembly, cell cycle regulation, and nucleolar function, providing insights into the molecular mechanisms underlying cellular growth and proliferation.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    B23/Nucleophosmin Antibody (7H10B9)

    sc-65943
    100 µg/ml
    $322.00

    Can sc-65943: B23 (7H10B9) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Cweed
    Thank you for your question. Yes, sc-65943: B23 (7H10B9) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 4 out of 5 by from Clear band observed in WB in HEL 92Clear band observed in WB in HEL 92.1.7 whole cell lysate. -SCBT QC
    Date published: 2015-02-19
    Rated 4 out of 5 by from Good specificity in WB in human B23 transfectedGood specificity in WB in human B23 transfected 293 whole cell lysate. -SCBT QC
    Date published: 2013-10-06
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    B23/Nucleophosmin Antibody (7H10B9) is rated 4.0 out of 5 by 2.
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