Date published: 2025-9-9
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Axl Antibody (H-3) - Western Blotting - Image 291025
Axl 抗体 (H-3): sc-166269. Caki-1 (A) 和 FHs 173We (B) 全细胞裂解液中 Axl 表达的 Western 印迹分析.
Axl 抗体 (H-3): sc-166269. Caki-1 (A) 和 FHs 173We (B) 全细胞裂解液中 Axl 表达的 Western 印迹分析.

Axl 抗体 (H-3): sc-166269

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寫評論提問

说明书
  • Axl 抗体 H-3 是小鼠单克隆 IgG1 κ，Axl抗体, 在29篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Axl的氨基酸771-894
  • Axl 抗体 (H-3) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Axl
  • 抗Axl抗体（H-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Axl (H-3): sc-166269的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Axl Antibody (H-3) for WB applications. 的首选辅助检测试剂，这些试剂现与Axl Antibody (H-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Axl抗体（H-3）是一种IgG1 κ小鼠单克隆Axl抗体（也称为AXL抗体或AXL受体酪氨酸激酶抗体），可通过WB、IP、IF和ELISA检测人源Axl蛋白。Axl抗体（H-3）既可以作为非偶联的抗Axl抗体形式提供，也可以作为多种偶联形式的抗Axl抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。受体酪氨酸激酶UFO家族由亚家族成员Rse（也称为Tyro3、Sky、Brt、Dtk、Etk2和Tif）、Axl（也称为UFO或ARK）和Mer（也称为Nyk或Eyk）组成。Rse主要在成年大脑中表达，在其他组织中的表达水平较低。Axl在心脏和骨骼肌中的表达水平最高。Mer已被鉴定为可能参与胶质母细胞瘤发展的酪氨酸激酶，它在卵巢、前列腺、肺和肾中的表达水平最高。生长停滞特异性基因Gas6和相关的抗凝因子蛋白质S已被鉴定为UFO家族受体的配体。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Axl 抗体 (H-3) 参考文献:

  1. 一种具有致癌潜力的新型假定酪氨酸激酶受体。  |  Janssen, JW., et al. 1991. Oncogene. 6: 2113-20. PMID: 1834974
  2. Axl抑制剂作为新型癌症治疗剂。  |  Shen, Y., et al. 2018. Life Sci. 198: 99-111. PMID: 29496493
  3. AXL作为乳腺癌治疗的目标。  |  Colavito, SA. 2020. J Oncol. 2020: 5291952. PMID: 32148495
  4. 免疫逃逸机制与AXL。  |  Son, HY. and Jeong, HK. 2021. Front Oncol. 11: 756225. PMID: 34778071
  5. AXL启动的旁分泌激活pSTAT3可增强肿瘤相关巨噬细胞的间充质和血管生成支持功能。  |  Hung, CN., et al. 2023. Cell Rep. 42: 113067. PMID: 37659081
  6. Axl和MerTK调节滑膜炎症，并通过抑制IL-6在类风湿性关节炎中起调节作用。  |  Nerviani, A., et al. 2024. Nat Commun. 15: 2398. PMID: 38493215
  7. rse是一种与Axl/Ufo同源的新型受体型酪氨酸激酶，在大脑中高水平表达。  |  Mark, MR., et al. 1994. J Biol Chem. 269: 10720-8. PMID: 7511603
  8. 抗凝因子蛋白 S 及其近亲 Gas6 是受体酪氨酸激酶 Tyro 3/Axl 家族的配体。  |  Stitt, TN., et al. 1995. Cell. 80: 661-70. PMID: 7867073

订购信息

产品名称产品编号规格价格数量收藏夹

Axl 抗体 (H-3)

sc-166269
200 µg/ml
$316.00

Axl (H-3): m-IgG Fc BP-HRP 套装

sc-527148
200 µg Ab; 10 µg BP
$354.00

Axl (H-3): m-IgG1 BP-HRP 套装

sc-532521
200 µg Ab; 20 µg BP
$354.00

Axl 抗体 (H-3) AC

sc-166269 AC
500 µg/ml, 25% agarose
$416.00

Axl 抗体 (H-3) HRP

sc-166269 HRP
200 µg/ml
$316.00

Axl 抗体 (H-3) FITC

sc-166269 FITC
200 µg/ml
$330.00

Axl 抗体 (H-3) PE

sc-166269 PE
200 µg/ml
$343.00

Axl 抗体 (H-3) Alexa Fluor® 488

sc-166269 AF488
200 µg/ml
$357.00

Axl 抗体 (H-3) Alexa Fluor® 546

sc-166269 AF546
200 µg/ml
$357.00

Axl 抗体 (H-3) Alexa Fluor® 594

sc-166269 AF594
200 µg/ml
$357.00

Axl 抗体 (H-3) Alexa Fluor® 647

sc-166269 AF647
200 µg/ml
$357.00

Axl 抗体 (H-3) Alexa Fluor® 680

sc-166269 AF680
200 µg/ml
$357.00

Axl 抗体 (H-3) Alexa Fluor® 790

sc-166269 AF790
200 µg/ml
$357.00

Does this antibody also recognize mouse Axl? Thanks

Asked by: Iker
Thank you for your question. We have not confirmed experimentally that this monoclonal, Axl Antibody (H-3): sc-166269, is reactive with mouse Axl. The antibody is raised against amino acids 771-894 of Axl of human origin, and the sequence shares 87% identity with mouse Axl. Thus, there is a good chance that the antibody would work for mouse. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to receive a sample for testing in your research. Please call us toll-free at (800) 457-3801 option 2, e-mail scbt@scbt.com, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-03-27

What is the recommended starting dilution for Western blot for Axl (H-3): sc-166269 monoclonal antibody?

Asked by: TinTin
Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in our recommended Western blot protocol, which you can find here: https://www.scbt.com/scbt/sv/resources/protocols/western-immuno-blotting
Answered by: Tech Service
Date published: 2017-01-30
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Rated 5 out of 5 by from AXLAXL AlexaFluor 647 antibody was used 1/100 diluted in D-PBS/Live-Dead stain (1:1000), supplemented with 60µg/ml human IgG (Sigma Aldrich) at 8◦C for 30 min. After that, cells were washed in FACS buffer (D-PBS/0.1% BSA), fixed in 4% PFA at room temperature (RT), and analyzed by flow cytometry.
Date published: 2023-01-16
Rated 4 out of 5 by from Good antibody for WBI tested this antibody diluted 1:500 in milk/TBST buffer, loading 40ug of total protein. The signal is good, clean bands without any background
Date published: 2022-07-05
Rated 5 out of 5 by from AXL for WBThe antibody is highly recommended for using in WB application. The signal is very strong, specific ad clear.
Date published: 2021-01-08
Rated 1 out of 5 by from Does not work for FCMThe AXL antibody does not work for flow cytometry
Date published: 2018-11-01
Rated 1 out of 5 by from Didn't work for us.This monoclonal Axl antibody(SC166269) didn't work as good as SC20741 we used before for last 5 years in our lab.
Date published: 2018-04-12
Rated 5 out of 5 by from Antibodies nicely working for WBImage shows analysis of AXL level in LN-229 cells upon silencing of AXL gene using siRNA. Axl Antibodies(H-3): sc-166269 detect a band of approximately 140 kDa (corresponding to molecular weight of AXL) and are specific.
Date published: 2018-02-23
Rated 1 out of 5 by from PostdocI have got the sample size of the antibody. Its did not work well compare to H-124 antibody. using H-124 antibody, I have got the specific intense band with in 1min exposure. But H-3 antibody is hard to detect specific band after 20 min of exposure.
Date published: 2017-04-07
Rated 5 out of 5 by from Strong AXL ab for WBNice strong WB band in HUV-EC-C whole cell lysate and A375 whole cell lysate.
Date published: 2017-01-10
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Axl 抗体 (H-3) is rated 3.7 out of 5 by 10.
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