Date published: 2025-9-11
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Aurora A Antibody (D-2) - Western Blotting - Image 150948
Aurora A 抗体 (D-2): sc-373856. XLK-WG 全细胞裂解液中极光 A 表达的 Western 印迹分析.
Aurora A Antibody (D-2) - Western Blotting - Image 150948
Aurora A 抗体 (D-2): sc-373856. XLK-WG 全细胞裂解液中极光 A 表达的 Western 印迹分析.
Aurora A 抗体 (D-2): sc-373856. XLK-WG 全细胞裂解液中极光 A 表达的 Western 印迹分析.

Aurora A 抗体 (D-2): sc-373856

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寫評論提問

说明书
  • Aurora A 抗体 D-2 是小鼠单克隆 IgG1 κ，Aurora A抗体, 在7篇文献中引用，规格为200 µg/ml
  • 与Xenopus laevis起源的Aurora A的C-terminus附近的肽图谱
  • 建议使用Aurora A抗体（D-2）通过WB, IP, IF和ELISA检测Aurora A的Xenopus和avian来源; 也和以下物种反应，包括： 和 equine, canine, bovine, porcine and avian
  • 抗Aurora A抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Aurora A (D-2): sc-373856的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Aurora A Antibody (D-2) for WB applications. 的首选辅助检测试剂，这些试剂现与Aurora A Antibody (D-2) 打包提供（请参阅下面的订购信息）。

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描述
说明书与实验方案
研究信息

関連項目

Aurora抗体

Aurora A抗体（D-2）是一种IgG1 κ小鼠单克隆Aurora A抗体（也称为Aurora A抗体），可通过WB、IP、IF和ELISA检测非洲爪蟾的Aurora A蛋白。Aurora A抗体（D-2）既可作为非共轭的抗Aurora A抗体形式，也可作为多种共轭的抗Aurora A抗体形式，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。致癌蛋白激酶Aurora A的激活调节真核细胞中的减数分裂和有丝分裂细胞周期。具体而言，Aurora A在G2/M进展中起关键作用。激活是通过自磷酸化发生的，虽然有14个位点受此影响，但只有位于295位的苏氨酸残基是活动所必需的。尽管自磷酸化介导激活，但许多其他蛋白质也会影响激活，包括纺锤体组装因子TPX2和p53。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Aurora A 抗体 (D-2) 参考文献:

  1. 章鱼卵提取物中的 pEg2 aurora-A 激酶, 组蛋白 H3 磷酸化和染色体组装  |  Scrittori, L., et al. 2001. J Biol Chem. 276: 30002-10. PMID: 11402032
  2. 鉴定章鱼极光-A 激酶 pEg2 中的功能性破坏盒。  |  Arlot-Bonnemains, Y., et al. 2001. FEBS Lett. 508: 149-52. PMID: 11707286
  3. 人极光A转录的细胞周期依赖性调控是由E4TF1的周期性抑制介导的。  |  Tanaka, M., et al. 2002. J Biol Chem. 277: 10719-26. PMID: 11790771
  4. 果蝇 Aurora A 激酶是 D-TACC 定位于中心体和调节星状微管所必需的。  |  Giet, R., et al. 2002. J Cell Biol. 156: 437-51. PMID: 11827981
  5. Aurora-A过表达揭示了四倍体化是p53-/-细胞中心体扩增的主要途径。  |  Meraldi, P., et al. 2002. EMBO J. 21: 483-92. PMID: 11847097
  6. Aurora A 激酶参与了爪蟾卵母细胞减数分裂 I-II 过程。  |  Castro, A., et al. 2003. J Biol Chem. 278: 2236-41. PMID: 12426316
  7. 鉴定章鱼 Aurora A 上的新型磷酸化位点并分析固定金属亲和层析法的磷酸肽富集。  |  Haydon, CE., et al. 2003. Mol Cell Proteomics. 2: 1055-67. PMID: 12885952
  8. 极光 A 和有丝分裂的承诺  |  Prigent, C. and Giet, R. 2003. Cell. 114: 531-2. PMID: 13678575

订购信息

产品名称产品编号规格价格数量收藏夹

Aurora A 抗体 (D-2)

sc-373856
200 µg/ml
$316.00

Aurora A (D-2): m-IgG Fc BP-HRP 套装

sc-527419
200 µg Ab; 10 µg BP
$354.00

Aurora A (D-2): m-IgG1 BP-HRP 套装

sc-532792
200 µg Ab; 20 µg BP
$354.00

Aurora A 抗体 (D-2) AC

sc-373856 AC
500 µg/ml, 25% agarose
$416.00

Aurora A 抗体 (D-2) HRP

sc-373856 HRP
200 µg/ml
$316.00

Aurora A 抗体 (D-2) FITC

sc-373856 FITC
200 µg/ml
$330.00

Aurora A 抗体 (D-2) PE

sc-373856 PE
200 µg/ml
$343.00

Aurora A 抗体 (D-2) Alexa Fluor® 488

sc-373856 AF488
200 µg/ml
$357.00

Aurora A 抗体 (D-2) Alexa Fluor® 546

sc-373856 AF546
200 µg/ml
$357.00

Aurora A 抗体 (D-2) Alexa Fluor® 594

sc-373856 AF594
200 µg/ml
$357.00

Aurora A 抗体 (D-2) Alexa Fluor® 647

sc-373856 AF647
200 µg/ml
$357.00

Aurora A 抗体 (D-2) Alexa Fluor® 680

sc-373856 AF680
200 µg/ml
$357.00

Aurora A 抗体 (D-2) Alexa Fluor® 790

sc-373856 AF790
200 µg/ml
$357.00

Aurora A (D-2) 中和勝肽

sc-373856 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-373856 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great with western blottingEven though it says it is not for human, we did a western and it recognized full length Aurora A in human cells and it was a single band. I recommend it.
Date published: 2018-11-08
Rated 4 out of 5 by from Good for WBThis Aurora A Antibody (D-2), sc-373856, recognizes a band of predicted M.W. on WB (HeLa Cell).
Date published: 2018-02-20
Rated 5 out of 5 by from 一般申请的抗体试用装，抗体按照说明书1：100稀释后使用，未在目的位置发现条带，但是在偏目的条带的上方20KD左右有条带，不知道是不是抗体特异性不好
Date published: 2018-01-10
Rated 3 out of 5 by from commonlyI use your antibody to do western blot. However there has some other brands except target brand.
Date published: 2017-09-25
Rated 4 out of 5 by from a relative good antibody for WBThis antibody recognizes a band of predicted M.W. on WB (1:500 dilution, 40 ug HeLa extract).
Date published: 2017-09-03
Rated 5 out of 5 by from it is goodi diluted this antibody with 1:500,and it is good!
Date published: 2017-08-29
Rated 5 out of 5 by from highly recommendedAs a user, I strongly recommend the need for a friend to use the product！
Date published: 2017-07-05
Rated 1 out of 5 by from Didnot work for WBNo bands were observed in MDA-MB-231 cell lysates. Used the ab at 1:1000 dilution.
Date published: 2017-07-03
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Aurora A 抗体 (D-2) is rated 4.3 out of 5 by 11.
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