Date published: 2025-9-7

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apoL1 抗体 (A-3): sc-390440

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说明书
  • apoL1 抗体 A-3 是小鼠单克隆 IgG1 κ,apoL1抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列201-285位于human物种的apoL1的内部区域内
  • apoL1 抗体 (A-3) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 apoL1
  • 抗apoL1抗体(A-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取apoL1 (A-3): sc-390440免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是apoL1 Antibody (A-3) for WB applications. 的首选辅助检测试剂,这些试剂现与apoL1 Antibody (A-3) 打包提供(请参阅下面的订购信息)。

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関連項目

apoL1抗体(A-3)是一种IgG1 κ小鼠单克隆apoL1抗体(也称为apoL1抗体),能够通过WB、IP、IF和ELISA检测到来源于人类的apoL1蛋白。apoL1抗体(A-3)既有无偶联的抗apoL1抗体形式,也有多种偶联形式的抗apoL1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。载脂蛋白是血浆脂蛋白的蛋白质组分。载脂蛋白L基因家族编码六种高度同源的蛋白质,分别命名为apoL-I至-VI,它们与大型高密度脂蛋白(HDL)相关。人类apoL家族定位于22q12.1-13.1染色体上的一个127,000-bp区域。ApoL已被鉴定为胰腺特异性蛋白,由383个氨基酸组成,包含一个12个氨基酸的分泌信号肽。apoL基因具有无TATA启动子,并含有推定的固醇调节元件,这表明这些基因的转录可能与低密度脂蛋白受体和涉及甘油三酯和胆固醇合成途径的基因相协调。在血管内皮细胞的动脉粥样硬化变化过程中,ApoL同源物的表达可以发生10倍的变化,这包括动脉粥样硬化病变的炎症反应。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

apoL1 抗体 (A-3) 参考文献:

  1. 体外对肿瘤坏死因子-α有反应的血管内皮基因在动脉粥样硬化病变中也有表达,其中包括凋亡抑制蛋白-1, 丹宁酸和两个新基因。  |  Horrevoets, AJ., et al. 1999. Blood. 93: 3418-31. PMID: 10233894
  2. 载脂蛋白 L 基因家族:组织特异性表达, 剪接, 启动子区域;发现一个新基因。  |  Duchateau, PN., et al. 2001. J Lipid Res. 42: 620-30. PMID: 11290834
  3. 人类载脂蛋白 L 基因簇:鉴定, 分类和分布地点。  |  Page, NM., et al. 2001. Genomics. 74: 71-8. PMID: 11374903
  4. 载脂蛋白 L 基因簇是最近在进化过程中出现的,并在人类血管组织中表达。  |  Monajemi, H., et al. 2002. Genomics. 79: 539-46. PMID: 11944986
  5. 载脂蛋白 L-I 是人类血清中的锥虫裂解因子。  |  Vanhamme, L., et al. 2003. Nature. 422: 83-7. PMID: 12621437
  6. 脂蛋白 L-I 和锥虫裂解活性在灵长类血清中的分布。  |  Poelvoorde, P., et al. 2004. Mol Biochem Parasitol. 134: 155-7. PMID: 14747153
  7. 载脂蛋白 L-I 与低 HDL 的 CAD 患者的高血糖和血浆甘油三酯呈正缔合作用。  |  Albert, TS., et al. 2005. J Lipid Res. 46: 469-74. PMID: 15604524
  8. 胰腺表达的一种新的人类高密度脂蛋白载脂蛋白 L。载脂蛋白 L 的鉴定, 克隆, 表征和血浆分布。  |  Duchateau, PN., et al. 1997. J Biol Chem. 272: 25576-82. PMID: 9325276

订购信息

产品名称产品编号规格价格数量收藏夹

apoL1 抗体 (A-3)

sc-390440
200 µg/ml
$316.00

apoL1 (A-3): m-IgG Fc BP-HRP 套装

sc-527631
200 µg Ab; 10 µg BP
$354.00

apoL1 (A-3): m-IgG1 BP-HRP 套装

sc-533004
200 µg Ab; 20 µg BP
$354.00

apoL1 抗体 (A-3) AC

sc-390440 AC
500 µg/ml, 25% agarose
$416.00

apoL1 抗体 (A-3) HRP

sc-390440 HRP
200 µg/ml
$316.00

apoL1 抗体 (A-3) FITC

sc-390440 FITC
200 µg/ml
$330.00

apoL1 抗体 (A-3) PE

sc-390440 PE
200 µg/ml
$343.00

apoL1 抗体 (A-3) Alexa Fluor® 488

sc-390440 AF488
200 µg/ml
$357.00

apoL1 抗体 (A-3) Alexa Fluor® 546

sc-390440 AF546
200 µg/ml
$357.00

apoL1 抗体 (A-3) Alexa Fluor® 594

sc-390440 AF594
200 µg/ml
$357.00

apoL1 抗体 (A-3) Alexa Fluor® 647

sc-390440 AF647
200 µg/ml
$357.00

apoL1 抗体 (A-3) Alexa Fluor® 680

sc-390440 AF680
200 µg/ml
$357.00

apoL1 抗体 (A-3) Alexa Fluor® 790

sc-390440 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for sc-390440: apoL1 (A-3) monoclonal antibody?

Asked by: TinTin
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2023-12-30
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Rated 4 out of 5 by from Effect of APOL1 disease risk variants on APOL1 genOur present study focuses on the impact of these variants on APOL1 mRNA transcription and translation. APOL1 plasmids (EV, G0, G1 and G2) were transfected into human embryonic kidney (HEK) 293T cells. APOL1 variant expression was observed to be significantly lower than that of APOL1G0. Podocyte cell lines stably expressing APOL1 transgenes also showed lower levels of APOL1 expression of APOL1 variants (G1 and G2) compared with APOL1G0 by Western blotting and FACS analysis. APOL1 mRNA transcript stability was tested after actinomycin D pulsing; APOL1G1 and APOL1G2 mRNAs transcript decayed 10-15% and 15-20% (within a period of 0.5-3 h) respectively. Our data suggest that down-regulated APOL1 protein expression in APOL1 variants is due to compromised transcription and decay of the APOL1 variant transcripts.
Date published: 2017-08-11
Rated 5 out of 5 by from It is good!I have been used this antibody for incubate the membrane, the result is good!
Date published: 2017-07-25
Rated 5 out of 5 by from worked well in western blotWe used this antibody for western blot and got a band around the correct molecular weight
Date published: 2017-01-16
Rated 4 out of 5 by from Good specificity and signal strength withGood specificity and signal strength with little background in human plasma. -SCBT QC
Date published: 2014-06-19
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apoL1 抗体 (A-3) is rated 4.5 out of 5 by 4.
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