Date published: 2025-9-6
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apoB Antibody (A-6) - Western Blotting - Image 297022
载脂蛋白 B 抗体 (A-6): sc-393636. 人肝脏组织提取物中载脂蛋白 B 表达的 Western 印迹分析 (A) 和人血浆中载脂蛋白 B 表达的 Western 印迹分析 (B).
载脂蛋白 B 抗体 (A-6): sc-393636. 人肝脏组织提取物中载脂蛋白 B 表达的 Western 印迹分析 (A) 和人血浆中载脂蛋白 B 表达的 Western 印迹分析 (B).

apoB 抗体 (A-6): sc-393636

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寫評論提問

说明书
  • apoB抗体(A-6)是小鼠单克隆IgG1 κ, 在29篇文献中引用，规格为200 µg/ml
  • 免疫human物种的apoB的氨基酸1-300
  • 抗-apoB 抗体 (A-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 apoB
  • 抗apoB抗体（A-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取apoB (A-6): sc-393636的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是apoB Antibody (A-6) for WB applications. 的首选辅助检测试剂，这些试剂现与apoB Antibody (A-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Apolipoprotein抗体，包括apoA-I, apoA-II, apoA-IV, apoA-V, apoC-I, apoC-II, apoC-III, apoC-IV, apoL1, apoL3, apoOL, apoB, apoD, apoE, apoF和apoH

apoB抗体（A-6）是一种IgG1 κ小鼠单克隆apoB抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的apoB。apoB抗体（A-6）既有非偶联形式的抗apoB抗体，也有多种偶联形式的抗apoB抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。载脂蛋白B（apoB）mRNA的转录后编辑由肝细胞中的APOBEC1（也称为人（或大鼠）小肠载脂蛋白B mRNA编辑蛋白，HEPR或REPR）调节，以实现编辑和未编辑RNA分子的稳态比例。已知有两种形式的apoB在哺乳动物的血浆中循环。apoB-100主要是在肝脏中合成的，作为极低密度脂蛋白颗粒的结构组分。apoB-100的截断形式apoB-48在小肠中合成，包含较大蛋白质的氨基末端2152个氨基酸。这种apoB产生的器官特异性分配是共同apoB基因RNA编辑的结果。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

apoB 抗体 (A-6) 参考文献:

  1. 含载脂蛋白 B-100 脂蛋白的组装和分泌的分子机制。  |  Borén, J., et al. 1991. Prog Lipid Res. 30: 205-18. PMID: 1823939
  2. 含载脂蛋白 B-100 的脂蛋白是血液循环中 3-碘甲腺原胺的主要载体。  |  Roy, G., et al. 2012. J Biol Chem. 287: 1790-800. PMID: 22128163
  3. 低密度脂蛋白结合区外的新型功能性 APOB 突变导致家族性高胆固醇血症。  |  Alves, AC., et al. 2014. Hum Mol Genet. 23: 1817-28. PMID: 24234650
  4. 针对载脂蛋白B-100的自身抗体是冠状动脉易损斑块的新标记。  |  Egusa, G. 2021. J Atheroscler Thromb. 28: 1020-1022. PMID: 33455997
  5. 由 APOB 基因变异导致的同卵家族性高胆固醇血症，临床过程不寻常。  |  Chlebus, K., et al. 2021. Kardiol Pol. 79: 1030-1031. PMID: 34125946
  6. APOB 基因表达作为冠状动脉粥样硬化严重程度标志物的重要性。  |  Owczarek, J., et al. 2023. Pol Arch Intern Med. 133: PMID: 37548372
  7. ApoA-I和ApoB水平以及ApoB与ApoA-I的比率可作为胃和食管胃连接部腺癌新辅助治疗病理形态反应的候选预处理生物标志物。  |  Nurczyk, K., et al. 2023. Pol Przegl Chir. 96: 44-49. PMID: 38629280
  8. 肾上腺皮质腺瘤患者的低密度脂蛋白颗粒和 APOB 浓度较高。  |  Sandooja, R., et al. 2024. J Clin Endocrinol Metab. 110: 195-207. PMID: 38870260
  9. 含载脂蛋白 B-100 的脂蛋白的组装和分泌。  |  Borén, J., et al. 1993. Biochem Soc Trans. 21: 487-93. PMID: 8359516
  10. 载脂蛋白 B 脂蛋白与蛋白聚糖结合的细胞后果。对动脉粥样硬化的潜在作用。  |  Hurt-Camejo, E., et al. 1997. Arterioscler Thromb Vasc Biol. 17: 1011-7. PMID: 9194748

订购信息

产品名称产品编号规格价格数量收藏夹

apoB 抗体 (A-6)

sc-393636
200 µg/ml
$316.00

apoB (A-6): m-IgG Fc BP-HRP 套装

sc-527703
200 µg Ab; 10 µg BP
$354.00

apoB (A-6): m-IgG1 BP-HRP 套装

sc-533076
200 µg Ab; 20 µg BP
$354.00

apoB 抗体 (A-6) AC

sc-393636 AC
500 µg/ml, 25% agarose
$416.00

apoB 抗体 (A-6) HRP

sc-393636 HRP
200 µg/ml
$316.00

apoB 抗体 (A-6) FITC

sc-393636 FITC
200 µg/ml
$330.00

apoB 抗体 (A-6) PE

sc-393636 PE
200 µg/ml
$343.00

apoB 抗体 (A-6) Alexa Fluor® 488

sc-393636 AF488
200 µg/ml
$357.00

apoB 抗体 (A-6) Alexa Fluor® 546

sc-393636 AF546
200 µg/ml
$357.00

apoB 抗体 (A-6) Alexa Fluor® 594

sc-393636 AF594
200 µg/ml
$357.00

apoB 抗体 (A-6) Alexa Fluor® 647

sc-393636 AF647
200 µg/ml
$357.00

apoB 抗体 (A-6) Alexa Fluor® 680

sc-393636 AF680
200 µg/ml
$357.00

apoB 抗体 (A-6) Alexa Fluor® 790

sc-393636 AF790
200 µg/ml
$357.00

What housekeeping marker you suggest as a western blot control for the Anti-apoB Antibody (A-6): m-IgG Fc BP-HRP bundle?

Asked by: NasimRostampour
Thank you for your questions. You can use the following high molecular weight markers 460 kDa “marker" = DNA-PKCS (G-4): sc-5282 300 kDa “marker” = p300 (F-4): sc-48343
Answered by: Santa Cruz TS
Date published: 2021-07-10

Can you explain how I can use Anti-apoB Antibody (A-6): m-IgG Fc BP-HRP Bundle expecificly? I know I dont need the secondary for this antibody but what is your recomendation for dilution and everything?

Asked by: Nasim88
For detailed information on assay specific dilutions and use, Please contact Technical Service.
Answered by: Tech Sales
Date published: 2021-05-04

Can it be used for Apo B100/48 specific detection?

Asked by: Stanley_S
Thank you for your question. Yes, this ApoB monoclonal antibody can detect ApoB and it's possible degradation products.
Answered by: Tech Service
Date published: 2025-06-13

Has this antibody been tested for detection of mouse ApoB? Does it recognize ApoB48?

Asked by: ZQChen
Thank you for your question. Yes, this antibody has been tested for detection of mouse apoB. There is Western blot data on our website in mouse plasma and liver tissue extracts. We do not know if the antibody can detect ApoB48.
Answered by: Technical Support
Date published: 2018-12-11

What is the recommended starting dilution for Western blot for sc-393636: apoB (A-6) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 2 out of 5 by from OK for ELISAUsed for ELISA against human ApoB-100 and it showed weak binding compared with another vendor's goat polyclonal antisera.
Date published: 2021-12-01
Rated 5 out of 5 by from Good antibodyThe apoB Antibody (A-6): sc-393636 works well for the detection of apoB of human origin via Western blot applications.
Date published: 2017-02-03
Rated 4 out of 5 by from Works great for human liver tissue extractWorks great for human liver tissue extract and apoB in human plasma by WB. -SCBT QC
Date published: 2013-06-17
Rated 5 out of 5 by from Good Western blot data of apoB expressionGood Western blot data of apoB expression in human liver tissue extract and apoB in human plasma . -SCBT QC
Date published: 2013-06-17
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apoB 抗体 (A-6) is rated 4.0 out of 5 by 4.
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