Date published: 2025-9-6
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Apolipoprotein A I/APOA1 Antibody (B-10) - Western Blotting - Image 159878
载脂蛋白 A-I 抗体 (B-10): sc-376818. 人血浆中载脂蛋白 A-I 的 Western 印迹分析.
载脂蛋白 A-I 抗体 (B-10): sc-376818. 人血浆中载脂蛋白 A-I 的 Western 印迹分析.

Apolipoprotein A I/APOA1 抗体 (B-10): sc-376818

4.5(10)
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说明书
  • Apolipoprotein A I/APOA1 抗体 B-10 是小鼠单克隆 IgG2b κ，Apolipoprotein A I/APOA1抗体, 在57篇文献中引用，规格为200 µg/ml
  • 免疫human物种的代表全长apoA-I的氨基酸1-267
  • Apolipoprotein A I/APOA1 抗体 (B-10) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 apoA-I
  • 抗Apolipoprotein A I/APOA1抗体（B-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Apolipoprotein A I/APOA1 (B-10): sc-376818的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 Apolipoprotein A I/APOA1 抗体 (B-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Apolipoprotein A I/APOA1 抗体 (B-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Apolipoprotein 抗体包括 apoA-I, apoA-II, apoA-IV, apoA-V, apoC-I, apoC-II, apoC-III, apoC-IV, apoL1 和 apoL3

apoA-I抗体（B-10）是一种IgG2b κ小鼠单克隆apoA-I抗体（也称为APOA1抗体或载脂蛋白A1抗体），它通过WB、IP、IF、IHC(P)和ELISA检测人源apoA-I蛋白。apoA-I抗体（B-10）既以非缀合的抗apoA-I抗体形式存在，也以多种缀合形式的抗apoA-I抗体存在，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®缀合物。载脂蛋白是血浆脂蛋白的蛋白质成分。人类apoA-I基因编码一条单链、243个氨基酸的蛋白质，该蛋白质促进胆固醇从组织流向肝脏以进行排泄。载脂蛋白A-I是血浆中高密度脂蛋白（HDL）的主要蛋白质成分。它可以作为卵磷脂胆固醇酰基转移酶（LCAT）的辅因子，后者负责形成大多数血浆胆固醇酯。人类apoA-II基因编码HDL颗粒中第二丰富的蛋白质，在那里它影响血浆中游离脂肪酸（FFA）的水平。人类apoA-IV基因编码一个由396个氨基酸组成的前体蛋白，该蛋白经过蛋白水解处理后与乳糜微粒一起从肠道分泌出来。ApoA-IV是体外LCAT的强效激活剂。人类apoA-V基因编码一个由366个氨基酸组成的蛋白质，被认为是血浆甘油三酯水平的重要决定因素。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Apolipoprotein A I/APOA1 抗体 (B-10) 参考文献:

  1. 高密度脂蛋白亚类和载脂蛋白 A-I。  |  Duriez, P. and Fruchart, JC. 1999. Clin Chim Acta. 286: 97-114. PMID: 10511287
  2. 鉴定介导细胞胆固醇外流并稳定 ATP 结合盒转运体 A1 的载脂蛋白 A-I 结构元素。  |  Natarajan, P., et al. 2004. J Biol Chem. 279: 24044-52. PMID: 15051721
  3. 载脂蛋白 A-I 刺激人巨噬细胞分泌载脂蛋白 E 与胆固醇外流无关。  |  Kockx, M., et al. 2004. J Biol Chem. 279: 25966-77. PMID: 15066991
  4. 规模效应：APOA1/C3/A4/A5 基因簇的变异。  |  Fullerton, SM., et al. 2004. Hum Genet. 115: 36-56. PMID: 15108119
  5. 多种罕见等全基因导致血浆中高密度脂蛋白胆固醇水平较低。  |  Cohen, JC., et al. 2004. Science. 305: 869-72. PMID: 15297675
  6. 不同大小的载脂蛋白 A-I 盘状高密度脂蛋白中铰链结构域的鉴定及其结构影响。  |  Maiorano, JN., et al. 2004. Biochemistry. 43: 11717-26. PMID: 15362856
  7. 匹伐他汀对 HepG2 细胞脂蛋白 A-I 生成的影响  |  Maejima, T., et al. 2004. Biochem Biophys Res Commun. 324: 835-9. PMID: 15474503
  8. 人类载脂蛋白 A-I N 端 (1-44) 结构域的构象和脂质结合。  |  Zhu, HL. and Atkinson, D. 2004. Biochemistry. 43: 13156-64. PMID: 15476409
  9. 载脂蛋白 E 同工型和载脂蛋白 AI 可防止淀粉样前体蛋白羧基末端片段相关的细胞毒性。  |  Maezawa, I., et al. 2004. J Neurochem. 91: 1312-21. PMID: 15584908
  10. 来自低HDL-C杂合子HDL中的ApoA-I突变L202P和K131del。  |  Ljunggren, S., et al. 2014. Proteomics Clin Appl. 8: 241-50. PMID: 24273187
  11. ApoA-I淀粉样变体的结构决定因素解释了为什么胆固醇代谢会得到改善，尽管HDL水平较低。  |  Del Giudice, R., et al. 2017. Biochim Biophys Acta Mol Basis Dis. 1863: 3038-3048. PMID: 28887204
  12. AIBP和APOA-I通过促进胆固醇外排协同抑制肠道肿瘤的生长和转移。  |  Zhang, T., et al. 2019. J Transl Med. 17: 161. PMID: 31101050

订购信息

产品名称产品编号规格价格数量收藏夹

Apolipoprotein A I/APOA1 抗体 (B-10)

sc-376818
200 µg/ml
$316.00

Apolipoprotein A I/APOA1 (B-10): m-IgG Fc BP-HRP 套装

sc-529941
200 µg Ab; 10 µg BP
$354.00

Apolipoprotein A I/APOA1 (B-10): m-IgGκ BP-HRP 套装

sc-523106
200 µg Ab, 40 µg BP
$354.00

Apolipoprotein A I/APOA1 抗体 (B-10) AC

sc-376818 AC
500 µg/ml, 25% agarose
$416.00

Apolipoprotein A I/APOA1 抗体 (B-10) HRP

sc-376818 HRP
200 µg/ml
$316.00

Apolipoprotein A I/APOA1 抗体 (B-10) FITC

sc-376818 FITC
200 µg/ml
$330.00

Apolipoprotein A I/APOA1 抗体 (B-10) PE

sc-376818 PE
200 µg/ml
$343.00

Apolipoprotein A I/APOA1 抗体 (B-10) Alexa Fluor® 488

sc-376818 AF488
200 µg/ml
$357.00

Apolipoprotein A I/APOA1 抗体 (B-10) Alexa Fluor® 546

sc-376818 AF546
200 µg/ml
$357.00

Apolipoprotein A I/APOA1 抗体 (B-10) Alexa Fluor® 594

sc-376818 AF594
200 µg/ml
$357.00

Apolipoprotein A I/APOA1 抗体 (B-10) Alexa Fluor® 647

sc-376818 AF647
200 µg/ml
$357.00

Apolipoprotein A I/APOA1 抗体 (B-10) Alexa Fluor® 680

sc-376818 AF680
200 µg/ml
$357.00

Apolipoprotein A I/APOA1 抗体 (B-10) Alexa Fluor® 790

sc-376818 AF790
200 µg/ml
$357.00

Do you know of any cases, where the antibody was used to bind to the ApoA1-derived membrane scaffold protein?

Asked by: Umbach27
Thank you for your question. We only have data for the detection of apoA-I of human origin in WB, IP, IF, IHC(P) and ELISA applications.
Answered by: Tech Support Europe
Date published: 2021-01-20

Is antigen retrieval required with working with paraffin-embedded sections?

Asked by: AbPolly
Thank you for your question. Yes, we recommend performing antigen retrieval when using this antibody for IHC(P). Our recommended method is citrate buffer, pH 6, and heat. Our full protocol can be found on the website here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-02-09

Can we purchase following two items from vendors in India right now? are these two items are available in santa cruz right now? sc-376818 and sc-516102

Asked by: saikatm91
Thank you for your question. This primary antibody and mouse binding protein are typically available in stock, however please inquire with our Customer Care Department for the most up to date information about product availability. Both products are currently available to be ordered through one of our authorized distributors in India. A link to search for contact information for our distributors is provided below. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-01-10

can sc-7297 is being uesed as secondery antibody for sc-376818

Asked by: saikatm91
Thank you for your question. No, it can not be used as a secondary antibody. sc-7297 is the catalog number for a mouse monoclonal primary antibody against HCAM (DF1485). And sc-376818 is the catalog number for a different mouse monoclonal primary antibody against apoA-I (B-10). A list of recommended secondary antibodies to be used with this primary antibody can be found on the product page. Choose which application you are using the primary antibody in, and a list of products is provided.
Answered by: Technical Support
Date published: 2017-01-05
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Rated 4 out of 5 by from APOA1This antibody is good,the bands were clear but had some unnecerssary bands
Date published: 2017-08-25
Rated 5 out of 5 by from Clean and good sensitivityProduced a single band at expected MW in HepG2 cell extract and human kidney lysate. No protein detected in HEK293, possibly the protein is not expressed in this cell line?
Date published: 2017-07-24
Rated 5 out of 5 by from Apolipoprotein A1 antibody for human samplesI used this antibody for the human samples and used for immunoblotting as well as IHC both i found nice results.
Date published: 2017-03-03
Rated 5 out of 5 by from functional abApoE in conditioned media of primary astrocytes from human apoE3/3 and apoE4/4 heterozygous mice was analyzed. Two bands were observed. These 2 bands were also observed when another anti-ApoE antibody (Non-sc) was used
Date published: 2017-02-16
Rated 5 out of 5 by from Good for detecting the human proteinA good choice for apoA detection. It provides clear and strong signal in blotting and staining procedures. This product has been published with.
Date published: 2017-01-27
Rated 1 out of 5 by from Could not detect anything on my blot or IPCould not detect anything on my blot or IP.
Date published: 2015-03-27
Rated 5 out of 5 by from Worked well in formalin fixedWorked well in formalin fixed, paraffin-embedded human kidney tissue for IHC. -SCBT QC
Date published: 2015-03-19
Rated 5 out of 5 by from Immunofluorescence staining of formalinImmunofluorescence staining of formalin-fixed HepG2 cells shows golgi apparatus localization. -SCBT QC
Date published: 2015-02-11
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Apolipoprotein A I/APOA1 抗体 (B-10) is rated 4.5 out of 5 by 10.
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