Date published: 2025-9-8
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Amphiregulin Antibody (G-4) - Western Blotting - Image 18475
Amphiregulin 抗体 (G-4): sc-74501. OV-90 全细胞裂解液 (A) 和大鼠卵巢组织提取物 (B) 中 Amphiregulin 表达的 Western 印迹分析.
Amphiregulin 抗体 (G-4): sc-74501. OV-90 全细胞裂解液 (A) 和大鼠卵巢组织提取物 (B) 中 Amphiregulin 表达的 Western 印迹分析.

Amphiregulin 抗体 (G-4): sc-74501

4.8(12)
寫評論提問

说明书
  • Amphiregulin 抗体 G-4 是小鼠单克隆 IgG1 κ，Amphiregulin抗体, 在29篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Amphiregulin的氨基酸1-155
  • Amphiregulin 抗体 (G-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Amphiregulin
  • 抗Amphiregulin抗体（G-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Amphiregulin (G-4): sc-74501的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Amphiregulin 抗体 (G-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Amphiregulin 抗体 (G-4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

Amphiregulin抗体（G-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Amphiregulin蛋白。抗Amphiregulin抗体（G-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Amphiregulin是表皮生长因子（EGF）家族的一员，在细胞信号传导中起着至关重要的作用，主要由角质形成细胞产生，在角质形成细胞中，Amphiregulin作为内源性生长因子发挥作用。Amphiregulin对于人体角质形成细胞的正常增殖至关重要，并与ErbB-1受体结合，后者对于皮肤、肺和胃肠道上皮组织的发育至关重要。研究表明，Amphiregulin的缺失会导致人类角质形成细胞端粒酶活性下降，这表明Amphiregulin在调节细胞衰老过程中起着重要作用。在研究中使用Amphiregulin单克隆抗体（G-4）可以深入了解上皮发育的机制以及Amphiregulin在各种生理和病理条件下的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Amphiregulin 抗体 (G-4) 参考文献:

  1. 衰老和细胞衰老对角质形成细胞端粒酶活性的影响  |  Matsui, M., et al. 2000. J Dermatol Sci. 22: 80-7. PMID: 10674820
  2. 表皮生长因子家族的新成员 Epiregulin 是正常人角质形成细胞中的一种自分泌生长因子。  |  Shirakata, Y., et al. 2000. J Biol Chem. 275: 5748-53. PMID: 10681561
  3. 物理医学培训。  |  TEGNER, WS. 1954. Acta Physiother Rheumatol Belg. 9: 49-52. PMID: 13180383
  4. 一种由硫酸肝素调控的人类角朊细胞自分泌因子与羊栖菜素相似或相同。  |  Cook, PW., et al. 1991. Mol Cell Biol. 11: 2547-57. PMID: 2017164
  5. 两性胰岛素在协调免疫、炎症和组织修复方面的新功能。  |  Zaiss, DMW., et al. 2015. Immunity. 42: 216-226. PMID: 25692699
  6. 巨噬细胞-细胞外基质轴通过安非他酮诱导的转化生长因子β活化来指导组织修复。  |  Minutti, CM., et al. 2019. Immunity. 50: 645-654.e6. PMID: 30770250
  7. Th17细胞衍生的安非拉酮通过激活肠肌成纤维细胞中的mTOR和MEK促进结肠炎相关性肠纤维化  |  Zhao, X., et al. 2023. Gastroenterology. 164: 89-102. PMID: 36113570
  8. 产生两性胰岛素的调节性 T 细胞对肺泡再生龛的调节。  |  Kaiser, KA., et al. 2023. J Exp Med. 220: PMID: 36534084
  9. 皮肤伤口愈合过程中 MR1 驱动信号和两性胰岛素对 MAIT 细胞的招募和修复功能的作用。  |  du Halgouet, A., et al. 2023. Immunity. 56: 78-92.e6. PMID: 36630919
  10. 来自调节性 T 细胞的两性胰岛素会促进非酒精性脂肪性肝炎的肝纤维化和胰岛素抵抗。  |  Savage, TM., et al. 2024. Immunity. 57: 303-318.e6. PMID: 38309273
  11. 中间肺泡干细胞中持续表达的两性胰岛素会导致进行性纤维化。  |  Zhao, R., et al. 2024. Cell Stem Cell. 31: 1344-1358.e6. PMID: 39096904
  12. 肝素结合型 EGF 样生长因子激活 HER4 可刺激趋化，但不能刺激增殖。  |  Elenius, K., et al. 1997. EMBO J. 16: 1268-78. PMID: 9135143

订购信息

产品名称产品编号规格价格数量收藏夹

Amphiregulin 抗体 (G-4)

sc-74501
200 µg/ml
$316.00

Amphiregulin (G-4): m-IgG Fc BP-HRP 套装

sc-528674
200 µg Ab; 10 µg BP
$354.00

Amphiregulin (G-4): m-IgGκ BP-HRP 套装

sc-521123
200 µg Ab, 40 µg BP
$354.00

Amphiregulin (G-4): m-IgG1 BP-HRP 套装

sc-543063
200 µg Ab; 20 µg BP
$354.00

Amphiregulin 抗体 (G-4) AC

sc-74501 AC
500 µg/ml, 25% agarose
$416.00

Amphiregulin 抗体 (G-4) HRP

sc-74501 HRP
200 µg/ml
$316.00

Amphiregulin 抗体 (G-4) FITC

sc-74501 FITC
200 µg/ml
$330.00

Amphiregulin 抗体 (G-4) PE

sc-74501 PE
200 µg/ml
$343.00

Amphiregulin 抗体 (G-4) Alexa Fluor® 488

sc-74501 AF488
200 µg/ml
$357.00

Amphiregulin 抗体 (G-4) Alexa Fluor® 546

sc-74501 AF546
200 µg/ml
$357.00

Amphiregulin 抗体 (G-4) Alexa Fluor® 594

sc-74501 AF594
200 µg/ml
$357.00

Amphiregulin 抗体 (G-4) Alexa Fluor® 647

sc-74501 AF647
200 µg/ml
$357.00

Amphiregulin 抗体 (G-4) Alexa Fluor® 680

sc-74501 AF680
200 µg/ml
$357.00

Amphiregulin 抗体 (G-4) Alexa Fluor® 790

sc-74501 AF790
200 µg/ml
$357.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Amphiregulin Antibody (G-4): sc-74501

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-09
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_74501, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Amphiregulin Regulates Melanocytic SenescenceEach Western blot was blocked for 1 h with 5% milk powder/TBS-T and incubated overnight at 4 °C with anti-AREG (1:500, Santa Cruz Biotech, Heidelberg,Germany).
Date published: 2021-03-24
Rated 5 out of 5 by from Good antibody!1:200 diluted for western blot using rat protein,the band is special and strong
Date published: 2019-08-18
Rated 4 out of 5 by from it gives good WB bandstested the sample on human aortic SMCs, and it gives sharp bands with right size.
Date published: 2017-07-19
Rated 5 out of 5 by from very good productIt is obvious that the member of the epidermal growth factor family, amphiregulin, was originally characterized as a Schwannoma cell-derived growth factor expressed in response to androgen in an SC2G mouse cell line, which we find very good.
Date published: 2017-02-02
Rated 5 out of 5 by from Great detection!Strong staining and clear band regardless of being used in mouse, rat, or human. This antibody will help you get great results. It has been cited several times.
Date published: 2017-01-17
Rated 5 out of 5 by from Wow!! Great IHCOutstanding IHC results; much superior to discontinued polyclonals
Date published: 2017-01-06
Rated 4 out of 5 by from WBThere was a 55kd WB band in human derived cells, but the theory molecular weight is 28kd, and I do not hnow why.
Date published: 2016-12-29
Rated 5 out of 5 by from Good publishable data in WB and IF in mouseGood publishable data in WB and IF in mouse mammary glands. -SCBT Publication Review
Date published: 2015-07-16
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_74501, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 16ms
  • REVIEWS, PRODUCT
Amphiregulin 抗体 (G-4) is rated 4.8 out of 5 by 12.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_12
  • loc_zh_CN, sid_74501, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 99ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。