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&alpha;<sub>1A</sub>-AR Antibody (4D8) - Western Blotting - Image 33665
α1A-AR (4D8): sc-100291. HL-60 全细胞裂解液中 α1A-AR 表达的 Western 印迹分析.
α1A-AR Antibody (4D8) - Western Blotting - Image 33665
α1A-AR (4D8): sc-100291. HL-60 全细胞裂解液中 α1A-AR 表达的 Western 印迹分析.
α1A-AR Antibody (4D8) - Western Blotting - Image 366640
α1A-AR 抗体 (4D8): sc-100291. HL-60 (A), PC-3 (B) 和 Hep G2 (C) 全细胞裂解液以及小鼠脑 (D) 和大鼠脑 (E) 组织提取物中 α1A-AR 表达的 Western 印迹分析.
α1A-AR (4D8): sc-100291. HL-60 全细胞裂解液中 α1A-AR 表达的 Western 印迹分析.

α1A-AR 抗体 (4D8): sc-100291

4.8(4)
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说明书
  • alpha 1A-AR 抗体 4D8 是小鼠单克隆 IgG1 κ，α1A-AR抗体, 在12篇文献中引用，规格为100 µg/ml
  • 针对human起源的α1A-AR的N-terminal区域
  • 推荐用于 mouse, rat 和 human 来源的α1A-AR WB, IP 和 ELISA检测
  • 关于如何获取α1A-AR (4D8): sc-100291的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成α1A-AR 抗体 (4D8)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

此外 AR 抗体包括 α1A-AR, α1D-AR, α2B-AR, β2-AR 和 β3-AR

α1A-AR抗体（4D8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的α1A-AR蛋白。抗α1A-AR抗体（4D8）以非结合形式提供。α1A-AR在交感神经系统起着关键作用，通过介导儿茶酚胺（如肾上腺素和去甲肾上腺素）的作用来调节心血管功能。α1A-AR与Gq/11蛋白结合，导致血管收缩和血压升高，影响血管张力和心输出量。α1A-AR的表达横跨心脏、大脑、肝脏和前列腺组织，其中ADRA1A基因的替代性剪接产生了多种具有独特C端的多态性，表现出不同的激活模式。心脏、肝脏和前列腺中的α1A-AR转录本长度从2.0到6.0kb不等，突出了其在生理过程中的重要作用以及在病理生理条件下的潜在影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

α1A-AR 抗体 (4D8) 参考文献:

  1. α-1-肾上腺素能受体亚型通过涉及 p27Kip1 的 cAMP 依赖性机制对转染 CHO 细胞的细胞周期进行不同程度的控制。  |  Shibata, K., et al. 2003. J Biol Chem. 278: 672-8. PMID: 12409310
  2. α1-肾上腺素能受体亚型对细胞周期的不同调节。  |  Gonzalez-Cabrera, PJ., et al. 2004. Endocrinology. 145: 5157-67. PMID: 15297446
  3. 依赖于β-阿司匹林的自发性α1a-肾上腺素受体内吞作用会导致α-受体阻滞剂通过循环区进行细胞内运输。  |  Pediani, JD., et al. 2005. Mol Pharmacol. 67: 992-1004. PMID: 15626751
  4. 哺乳动物甲胎蛋白会改变α（1a）-肾上腺素能受体的亚细胞定域, 内化和信号传导。  |  Xu, Q., et al. 2006. Mol Pharmacol. 70: 532-41. PMID: 16690783
  5. 实时检测单个活细胞在苯肾上腺素刺激下的α1A-AR 运动。  |  Xu, N., et al. 2007. Acta Pharmacol Sin. 28: 796-802. PMID: 17506938
  6. 终末期肾病患者网膜动脉中α1-肾上腺素受体功能变化的证据。  |  Cruz-Domínguez, MP., et al. 2008. Auton Autacoid Pharmacol. 28: 19-27. PMID: 18257748
  7. 通过共聚焦显微镜和图像分析对少突胶质细胞瘤进行 GFAP 和 alpha1a-AR 染色以及核形态测量：预测少突胶质细胞瘤存活率的有用参数。  |  Moro-Rodríguez, E., et al. 2008. Diagn Pathol. 3 Suppl 1: S26. PMID: 18673515
  8. α1-肾上腺素受体药物组：下尿路中的α1L-肾上腺素受体和α1A-肾上腺素受体。  |  Nishimune, A., et al. 2010. Int J Urol. 17: 31-7. PMID: 19694838
  9. 衰老雌性小鼠的颈动脉功能障碍是由α;1A肾上腺素受体活性增加和COX衍生的血管收缩剂前列腺素介导的。  |  Costa, TJ., et al. 2023. Am J Physiol Heart Circ Physiol. 324: H417-H429. PMID: 36705993
  10. 人类α 1C-肾上腺素能受体 cDNA 的克隆, 功能表达和组织分布。  |  Hirasawa, A., et al. 1993. Biochem Biophys Res Commun. 195: 902-9. PMID: 8396931
  11. 新型人类 alpha1A 肾上腺素受体异构体的分子克隆, 基因组特征和表达。  |  Chang, DJ., et al. 1998. FEBS Lett. 422: 279-83. PMID: 9490024
  12. 人体输尿管中α1肾上腺素受体亚型的研究。  |  Itoh, Y., et al. 2007. Int J Urol. 14: 749-53. PMID: 17681068

订购信息

产品名称产品编号规格价格数量收藏夹

α1A-AR 抗体 (4D8)

sc-100291
100 µg/ml
$333.00

How selective is this antibody for alpha-1A? We need to address the selectivity issue in light of Michel et al. (2009) &quot;How reliable are G-protein-coupled receptor antibodies?&quot; NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY 379: 386-388.

Asked by: cpoon
Thank you for your question. The manuscript you mention recommends validating by IHC, but I would like to aslso suggest that also using siRNAs and CRISPR followed by a WB would also provide validation results to determine the reliability of the antibody. You can find recommended gene silencing products on the antibody product block: https://www.scbt.com/scbt/product/alpha1a-ar-antibody-4d8?requestFrom=search If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email &lt;scbt@scbt.com&gt;, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-06-28

For Western Blot, is it recommended to use denatured or non-denatured conditions with &alpha;1A-AR (4D8): sc-100291 antibody?

Asked by: Germaine
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Great for Western BlotAntibody works well in human cell lines HL-60, PC-3, and HepG2, and in mouse and rat brain tissue extracts. -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from Worked very wellI used this antibody on mice tissues (liver, muscle, aorta) and it worked very well.
Date published: 2018-04-06
Rated 5 out of 5 by from positive resultsProduct sc-100291 is used for kinase assay using human myeloid cells,positive results!
Date published: 2016-12-17
Rated 4 out of 5 by from Distinct band observed by WB using HLDistinct band observed by WB using HL-60 whole cell lysate. -SCBT QC
Date published: 2014-02-27
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α 1A -AR 抗体 (4D8) is rated 4.8 out of 5 by 4.
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