Date published: 2025-9-6
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alpha Tubulin Antibody (YOL1/34) - Western Blotting - Image 16435
α 管突蛋白 (YOL1/34): sc-53030. NIH/3T3 (A), HeLa (B), A-431 (C), K-562 (D), PC-12 (E) 和 SW-13 (F) 全细胞裂解液中微管蛋白表达的 Western 印迹分析.
α 管突蛋白 (YOL1/34): sc-53030. NIH/3T3 (A), HeLa (B), A-431 (C), K-562 (D), PC-12 (E) 和 SW-13 (F) 全细胞裂解液中微管蛋白表达的 Western 印迹分析.

alpha Tubulin 抗体 (YOL1/34): sc-53030

4.9(9)
寫評論提問

说明书
  • alpha Tubulin 抗体 YOL1/34 是大鼠单克隆 IgG2a, 在88篇文献中引用，提供 200 µg/ml
  • 全长纯化的Saccharomyces cerevisiae来源的α Tubulin
  • alpha Tubulin 抗体 (YOL1/34) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 yeast来源的α Tubulin
  • 抗alpha Tubulin抗体（YOL1/34）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取alpha Tubulin (YOL1/34): sc-53030的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成alpha Tubulin 抗体 (YOL1/34)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Tubulin抗体，包括α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

α Tubulin 抗体（YOL1/34）是一种鼠单克隆 IgG2a 抗体，可识别小鼠、大鼠、人类和酵母中的 α Tubulin，即细胞骨架的关键结构蛋白。α Tubulin 单克隆抗体（YOL1/34）针对从酿酒酵母（酵母）中提取的全长纯化 α Tubulin 制备，适用于多种技术 ，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。α微管蛋白与β微管蛋白形成异二聚体，并组装成微管，微管对于细胞过程至关重要，例如维持细胞形状、细胞内运输和细胞分裂过程中的纺锤体形成。微管作为动态结构，对于细胞运动、信号传导和细胞组织等多种功能至关重要。α Tubulin (YOL1/34) 单克隆抗体还可用于检测翻译后修饰，例如酪氨酸化，后者通过影响微管的稳定性和组装来调节微管的动态。抗-α Tubulin 抗体 (YOL1/34) 可检测来自不同物种的 α Tubulin，因此是跨物种微管行为比较研究的宝贵工具。此外，α Tubulin 存在多种同工型，每种同工型在不同细胞环境中都会对微管的功能多样性产生影响。抗-α Tubulin 抗体（YOL1/34）有非结合型和结合型两种形式，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物，为各种实验设置提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

alpha Tubulin 抗体 (YOL1/34)

sc-53030
200 µg/ml
$316.00

alpha Tubulin 抗体 (YOL1/34) AC

sc-53030 AC
500 µg/ml, 25% agarose
$416.00

alpha Tubulin 抗体 (YOL1/34) HRP

sc-53030 HRP
200 µg/ml
$316.00

alpha Tubulin 抗体 (YOL1/34) FITC

sc-53030 FITC
200 µg/ml
$330.00

alpha Tubulin 抗体 (YOL1/34) PE

sc-53030 PE
200 µg/ml
$343.00

alpha Tubulin 抗体 (YOL1/34) Alexa Fluor® 488

sc-53030 AF488
200 µg/ml
$357.00

alpha Tubulin 抗体 (YOL1/34) Alexa Fluor® 546

sc-53030 AF546
200 µg/ml
$357.00

alpha Tubulin 抗体 (YOL1/34) Alexa Fluor® 594

sc-53030 AF594
200 µg/ml
$357.00

alpha Tubulin 抗体 (YOL1/34) Alexa Fluor® 647

sc-53030 AF647
200 µg/ml
$357.00

alpha Tubulin 抗体 (YOL1/34) Alexa Fluor® 680

sc-53030 AF680
200 µg/ml
$357.00

alpha Tubulin 抗体 (YOL1/34) Alexa Fluor® 790

sc-53030 AF790
200 µg/ml
$357.00

Salve a tutti, mi consigliereste un secondario compatibile con questo anticorpo?

Asked by: Sss89
Grazie per la sua domanda. Al momento non offriamo anticorpi secondari o proteine di legame specifici contro gli anticorpi primari di ratto. Per ulteriori informazioni, contattare il nostro servizio tecnico. "What secondary antibody is compatible with this antibody?" Thank you for your question. Unfortunately, we currently do not offer any secondary antibodies or binding proteins specific against rat primary antibodies. Please contact our technical service if you require additional information.
Answered by: Tech Support Europe
Date published: 2019-07-23

Hi, I would like to request a 10 microgram sample of α Tubulin (YOL1/34): sc-53030 for use with western blots of yeast extracts. Thanks! Sincerely, Telsa

Asked by: Telsa
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample, e-mail <scbt@scbt.com>.
Answered by: Technical Support 15
Date published: 2017-12-15

Where does the epitope of YOL1/34 antibody bind on the tubulin? Does it bind to the folded or unfolded protein?  Thanks

Asked by: Emmanuel Nsamba
Thank you for your question. This rat monoclonal antibody, α Tubulin (YOL1/34): sc-53030, is raised against full length purified alpha tubulin of Saccharomyces cerevisiae origin. The exact epitope recognized by the antibody has not yet been determined. We have data using the antibody for detection of the denatured (Western blot) protein, as well as the native protein (IF and IHC(P)).
Answered by: Technical Support
Date published: 2022-02-24

Can α Tubulin Antibody (YOL1/34): sc-53030 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, α Tubulin Antibody (YOL1/34): sc-53030 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 14
Date published: 2017-01-09
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Rated 5 out of 5 by from YOL1/34 anti-α-tubulinAn excellent, reliable, multi-species convenient antibody for microtubule imaging. Especially effective for whole-mount immunofluorescence in plant samples.
Date published: 2024-04-02
Rated 5 out of 5 by from Label osteoclast microtubulesGuimbal, S. et al. (PubMed 31461543) used alpha Tubulin (YOL1/34) antibody to label microtubules in osteoclasts.
Date published: 2021-09-22
Rated 5 out of 5 by from anti-α-tubulinExcellent monoclonal antibody for the detection of α-tubulin of yeast and human origin. Recommended dilution: 1/1000
Date published: 2019-02-11
Rated 5 out of 5 by from Great for cell homogenates.It did not work for whole tissue homogentes, but it had a great signal for whole cell homogenates.
Date published: 2018-01-31
Rated 5 out of 5 by from Great Antibody for Staining and IFThis antibody works very well for a variety of applications including WB, IF, and even IHC. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, yeast, and human samples.
Date published: 2016-12-28
Rated 5 out of 5 by from It seems to work well so far in WBIt seems to work well so far in WB.
Date published: 2015-09-16
Rated 4 out of 5 by from Used on human samples in WBUsed on human samples in WB, saw band of interest.
Date published: 2015-09-14
Rated 5 out of 5 by from Beautiful IF dataBeautiful IF data, specific staining of cytoplasm.
Date published: 2015-03-20
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alpha Tubulin 抗体 (YOL1/34) is rated 4.9 out of 5 by 9.
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