Date published: 2025-9-6
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alpha Tubulin Antibody (YL1/2) - Western Blotting - Image 372996
α微管蛋白抗体 (YL1/2): sc-53029. NIH/3T3 (A), RAW 264. 7 (B), C3H/10T1/2 (C), Jurkat (D), A-10 (E) 和 K-562 (F) 全细胞裂解液中 α 管蛋白表达的 Western 印迹分析.
α微管蛋白抗体 (YL1/2): sc-53029. NIH/3T3 (A), RAW 264. 7 (B), C3H/10T1/2 (C), Jurkat (D), A-10 (E) 和 K-562 (F) 全细胞裂解液中 α 管蛋白表达的 Western 印迹分析.

alpha Tubulin 抗体 (YL1/2): sc-53029

4.6(10)
寫評論提問

说明书
  • alpha Tubulin 抗体 YL1/2 是大鼠单克隆 IgG2a, 在90篇文献中引用，提供 200 µg/ml
  • 全长纯化 α Tubulin（Saccharomyces cerevisiae来源
  • alpha Tubulin 抗体 (YL1/2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和 yeast来源的α Tubulin; 还建议用于检测the tyrosinated form of α Tubulin
  • 抗alpha Tubulin抗体（YL1/2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取alpha Tubulin (YL1/2): sc-53029的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成alpha Tubulin 抗体 (YL1/2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体 包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

α Tubulin 抗体（YL1/2）是一种鼠单克隆 IgG2a 抗体，针对来自酿酒酵母（酵母）的全长纯化 α Tubulin 产生。α Tubulin（YL1/2）单克隆抗体可检测来自不同物种的 α Tubulin，包括小鼠、大鼠、人类和酵母，应用范围包括蛋白质印迹（ WB)、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）等多种应用中检测包括小鼠、大鼠、人类和酵母在内的多种物种的α微管蛋白。α微管蛋白是微管网络的核心组成部分，是细胞骨架的重要组成部分，参与维持细胞形状、细胞内运输和有丝分裂期间染色体的正确分离。微管由α和β微管蛋白异二聚体组成，可组装成动态结构，为细胞提供机械支撑，并促进细胞分裂和运动等多种细胞过程。抗α微管蛋白抗体（YL1/2）还可识别α微管蛋白的酪氨酸化形式，这种翻译后修饰在调节微管稳定性和新微管的组装方面发挥着重要作用。酪氨酸化和脱酪氨酸化循环对于控制微管动力学至关重要，会影响细胞功能，如信号传导和运动。了解α微管蛋白在细胞内的定位和调节对于研究细胞组织、微管功能和细胞周期过程的调节至关重要。α Tubulin (YL1/2) 抗体有非结合型和结合型两种形式（包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种 Alexa Fluor® 结合物），为各种实验技术提供了多功能工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

alpha Tubulin 抗体 (YL1/2)

sc-53029
200 µg/ml
$316.00

alpha Tubulin 抗体 (YL1/2) AC

sc-53029 AC
500 µg/ml, 25% agarose
$416.00

alpha Tubulin 抗体 (YL1/2) HRP

sc-53029 HRP
200 µg/ml
$316.00

alpha Tubulin 抗体 (YL1/2) FITC

sc-53029 FITC
200 µg/ml
$330.00

alpha Tubulin 抗体 (YL1/2) PE

sc-53029 PE
200 µg/ml
$343.00

alpha Tubulin 抗体 (YL1/2) Alexa Fluor® 488

sc-53029 AF488
200 µg/ml
$357.00

alpha Tubulin 抗体 (YL1/2) Alexa Fluor® 546

sc-53029 AF546
200 µg/ml
$357.00

alpha Tubulin 抗体 (YL1/2) Alexa Fluor® 594

sc-53029 AF594
200 µg/ml
$357.00

alpha Tubulin 抗体 (YL1/2) Alexa Fluor® 647

sc-53029 AF647
200 µg/ml
$357.00

alpha Tubulin 抗体 (YL1/2) Alexa Fluor® 680

sc-53029 AF680
200 µg/ml
$357.00

alpha Tubulin 抗体 (YL1/2) Alexa Fluor® 790

sc-53029 AF790
200 µg/ml
$357.00

I have a question concering α Tubulin Antibody (YL1/2): sc-53029. I would like to know whether it reacts with α Tubulin in Drosophila melanogaster in case of immunostaining and gives positive result?

Asked by: Amid
Thank you for your question. We do not have any information on whether this works on Drosophila melanogaster samples (we do know it works on mouse, rat, human, yeast and dog, so it is possible it works fine). Unfortunately we do not have a sample of this antibody, but we do offer a sample of another alpha tubulin antibody, sc-8035. If you would like to try a sample of that, please contact our European Technical Service team, they would be happy to help you! You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-26

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 2
Date published: 2017-01-10
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Rated 4 out of 5 by from Specific signal by western blotWe used this antibody on human cultured tenocytes and we obtained a clear band at the expected molecular weight
Date published: 2020-10-12
Rated 5 out of 5 by from Works well in mouse keratinocytes!Our lab uses alpha Tubulin antibody in mouse keratinocytes. It works well when fixed using Methanol at 1:100. We use this antibody often and it looks great!
Date published: 2020-10-01
Rated 5 out of 5 by from Specific bandsSpecific bands at 55kda, 1:800 used for mouse spleen tissue ,I got ideal result always use this antibody.
Date published: 2017-07-29
Rated 5 out of 5 by from Western blotWestern blot using mice brain tissue (60ug) at 1:500 dilution over night incubation for 4℃，nice bands seen.
Date published: 2017-05-09
Rated 5 out of 5 by from Outstanding for all applicationsTake a look at WB,IF and IHC results; great data!!
Date published: 2017-01-11
Rated 5 out of 5 by from Reliable Antibody, Great for IHCThis antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC. Clear signal with little to no background. Beautiful tissue staining. Works well in a variety of species including mouse, rat, yeast, and human samples.
Date published: 2017-01-04
Rated 5 out of 5 by from immunofluorecence in cultured cells worksimmunofluorecence in cultured cells works really well use at 1:100 in Methanol fix. Also works well in Tissue such as mouse skin and intestinal epithelium.
Date published: 2016-07-27
Rated 5 out of 5 by from The antibody works well for both WB and ICCThe antibody works well for both WB and ICC.
Date published: 2015-10-02
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alpha Tubulin 抗体 (YL1/2) is rated 4.6 out of 5 by 10.
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