Date published: 2025-9-6
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alpha Tubulin Antibody (B-5-1-2) - Western Blotting - Image 375360
α微管蛋白抗体 (B-5-1-2): sc-23948. NIH/3T3 (A), 3T3-L1 (B), Sol8 (C), C2C12 (D), SJRH30 (E) 和 A-10 (F) 全细胞裂解液中 α 管蛋白表达的 Western 印迹分析.
α微管蛋白抗体 (B-5-1-2): sc-23948. NIH/3T3 (A), 3T3-L1 (B), Sol8 (C), C2C12 (D), SJRH30 (E) 和 A-10 (F) 全细胞裂解液中 α 管蛋白表达的 Western 印迹分析.

alpha Tubulin 抗体 (B-5-1-2): sc-23948

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说明书
  • alpha Tubulin抗体(B-5-1-2)是小鼠单克隆IgG1 κ, 在574篇文献中引用，规格为200 µg/ml
  • 针对Strongylocentrotus purpuratus（海胆）精子轴丝中的抗Sarkosyl丝带
  • alpha Tubulin 抗体 (B-5-1-2) 推荐用于WB, IP, IF 和 IHC(P)，检测multiple 来源的 α Tubulin
  • 抗alpha Tubulin抗体（B-5-1-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取alpha Tubulin (B-5-1-2): sc-23948的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 alpha Tubulin 抗体 (B-5-1-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与alpha Tubulin 抗体 (B-5-1-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Tubulin 抗体 包括 α 1 Tubulin, α 3 Tubulin, α 4 Tubulin, α Tubulin, β Tubulin, β 2 Tubulin, β 3 Tubulin, δ Tubulin, ε Tubulin和γ Tubulin

α Tubulin 抗体 (B-5-1-2) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，针对来自 Strongylocentrotus purpuratus（海胆）精子轴丝的抗 Sarkosyl 抗性丝带。抗-α Tubulin 抗体 (B -5-1-2）可检测包括人类、小鼠、大鼠等在内的多种物种的α微管蛋白，可用于蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）以及石蜡包埋切片免疫组织化学（IHCP）等应用。微管蛋白是细胞骨架中一种重要的结构蛋白，有多种同工型，包括α、β、γ、δ和ε，它们构成微管的基础，对于细胞分裂、细胞内运输和维持细胞形状等过程至关重要。α微管蛋白与β微管蛋白形成异二聚体，组装成微管，为细胞结构提供支架，并促进有丝分裂期间染色体的正确排列和分离。α-微管蛋白的翻译后修饰（包括乙酰化、脱酪氨酸化和磷酸化）在调节微管稳定性和动力学方面发挥着重要作用，影响细胞运动和信号传导等过程。α-微管蛋白单克隆抗体（B-5-1-2）适用于研究微管蛋白的结构作用、翻译后修饰、细胞骨架动力学和细胞调控的研究人员。α Tubulin (B-5-1-2) 抗体有非结合型和结合型两种形式，包括辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）、琼脂糖和多种 Alexa Fluor® 结合物，为各种实验设置提供了灵活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

alpha Tubulin 抗体 (B-5-1-2) 参考文献:

  1. 培养的大鼠肾间质细胞核中存在βII异型小管蛋白。  |  Walss, C., et al. 1999. Cell Motil Cytoskeleton. 42: 274-84. PMID: 10223634
  2. 鉴定大西洋鳕鱼（Gadus morhua）冷适应微管中的βIII-和βIV-微管蛋白异型：抗体图谱和 cDNA 测序。  |  Modig, C., et al. 1999. Cell Motil Cytoskeleton. 42: 315-30. PMID: 10223637
  3. Delta-tubulin和epsilon-tubulin：两种新的人类中心体管蛋白揭示了中心体结构和功能的新方面。  |  Chang, P. and Stearns, T. 2000. Nat Cell Biol. 2: 30-5. PMID: 10620804
  4. 人脑中具有管蛋白免疫反应的神经元核内包涵体。  |  Woulfe, J. and Munoz, D. 2000. Neuropathol Appl Neurobiol. 26: 161-71. PMID: 10840279
  5. α-, β-和γ-管蛋白：序列比较和结构限制。  |  Burns, RG. 1991. Cell Motil Cytoskeleton. 20: 181-9. PMID: 1773446
  6. γ-微管蛋白存在于黑腹果蝇和智人体内，与中心体有关。  |  Zheng, Y., et al. 1991. Cell. 65: 817-23. PMID: 1904010
  7. α-微管蛋白Ser165的磷酸模拟突变可促进微管中GTP帽的持久性。  |  Maddula, V., et al. 2022. Biochemistry. 61: 1508-1516. PMID: 35799350
  8. 隐球菌与α-微管蛋白和相关皮罗宁类似物的分子对接。  |  Vergoten, G. and Bailly, C. 2023. Plants (Basel). 12: PMID: 36679009
  9. 特约评论：三磷酸核苷酸在肌动蛋白和微管蛋白组装和功能中的作用。  |  Weisenberg, RC. 1981. Cell Motil. 1: 485-97. PMID: 6756643
  10. 多种形式的微管蛋白：不同的基因产物和共价修饰。  |  Ludueña, RF. 1998. Int Rev Cytol. 178: 207-75. PMID: 9348671
  11. 氨基端和羧基端γ-和α-微管蛋白突变体在培养上皮细胞中的表达。  |  Leask, A. and Stearns, T. 1998. J Biol Chem. 273: 2661-8. PMID: 9446570
  12. Rbl2p-β-微管蛋白复合物的形成和功能。  |  Archer, JE., et al. 1998. Mol Cell Biol. 18: 1757-62. PMID: 9488492

订购信息

产品名称产品编号规格价格数量收藏夹

alpha Tubulin 抗体 (B-5-1-2)

sc-23948
200 µg/ml
$316.00

alpha Tubulin (B-5-1-2): m-IgG Fc BP-HRP 套装

sc-526639
200 µg Ab; 10 µg BP
$354.00

alpha Tubulin (B-5-1-2): m-IgG1 BP-HRP 套装

sc-532012
200 µg Ab; 20 µg BP
$354.00

alpha Tubulin 抗体 (B-5-1-2) AC

sc-23948 AC
500 µg/ml, 25% agarose
$416.00

alpha Tubulin 抗体 (B-5-1-2) HRP

sc-23948 HRP
200 µg/ml
$316.00

alpha Tubulin 抗体 (B-5-1-2) FITC

sc-23948 FITC
200 µg/ml
$330.00

alpha Tubulin 抗体 (B-5-1-2) PE

sc-23948 PE
200 µg/ml
$343.00

alpha Tubulin 抗体 (B-5-1-2) Alexa Fluor® 488

sc-23948 AF488
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-5-1-2) Alexa Fluor® 546

sc-23948 AF546
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-5-1-2) Alexa Fluor® 594

sc-23948 AF594
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-5-1-2) Alexa Fluor® 647

sc-23948 AF647
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-5-1-2) Alexa Fluor® 680

sc-23948 AF680
200 µg/ml
$357.00

alpha Tubulin 抗体 (B-5-1-2) Alexa Fluor® 790

sc-23948 AF790
200 µg/ml
$357.00

What buffer would I recommend to use to dilute this product? IF recommended concentration？

Asked by: ZSY2022
Thank you for your question. It would be helpful if you could email us directly at asia@scbio.cn. So we can have a more interactive discussion about this and other related issues.
Answered by: Zhanglong
Date published: 2022-06-08

Has α Tubulin Antibody (B-5-1-2): sc-23948 been used for brain sections?

Asked by: Alex3
Thank you for your question. This antibody is fine for mouse tissue sections, and should work great on your mouse brain samples. If you have any further questions, please reach out to Technical support at scbt@scbt.com, or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

I am working with mouse tissue, but need to perform immunofluorescence. Is this possible with α Tubulin Antibody (B-5-1-2)?

Asked by: Trav11
If working with mouse tissue, you will need to use one of our mouse monoclonal primary antibodies that are directly conjugated. The α Tubulin Antibody (B-5-1-2): sc-23948 is not currently available with direct conjugation. But, we have an alternative, α Tubulin Antibody (TU-02): sc-8035, that is available directly conjugated to HRP (sc-8035 HRP), phycoerythrin (sc-8035 PE), fluorescein (sc-8035 FITC), Alexa Fluor® 488 (sc-8035 AF488) or Alexa Fluor® 647 (sc-8035 AF647). This product can be found at the following link: https://www.scbt.com/scbt/product/alpha-tubulin-antibody-tu-02
Answered by: Tech Service 8
Date published: 2016-12-22
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Rated 5 out of 5 by from Muy buen reconocimiento de la proteínaLo ultilice para WB y obtuve una banda muy clara al poco tiempo de revelado
Date published: 2023-10-03
Rated 4 out of 5 by from Good antibodiesThis antibody works well in western blot of human whole cell extracts.
Date published: 2021-05-31
Rated 5 out of 5 by from A bit weaker than DM1A but equally goodExcellent for WB and IF, using saponin for permeabilization in IF.
Date published: 2020-11-20
Rated 5 out of 5 by from Work well as internal controlThe band developed in WB was clear and consistent. We are satisfactory for its performance as an internal control.
Date published: 2020-09-01
Rated 5 out of 5 by from Wonderful housekeeping gene, loading controlConsistently smooth, clean bands at 55kDa are observed, serving as a loading control and marker of cytoskeleton elements (microtubule) in various human cell lines.
Date published: 2018-07-12
Rated 5 out of 5 by from good antibodyI tried it for IF. Good signals although they were slightly weaker than DM1A's.
Date published: 2018-06-18
Rated 5 out of 5 by from confirmed for ICC, IHC, WBThis antibody works very well for immunocytochemistry, immunohistochemistry (frozen sections) and western blots.
Date published: 2017-11-12
Rated 5 out of 5 by from Used in WBstrong signal for WB, we usually use the dilution 1:500
Date published: 2017-08-17
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alpha Tubulin 抗体 (B-5-1-2) is rated 4.9 out of 5 by 24.
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