Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

α-Adaptin 1/2 抗体 (C-8): sc-17771

4.9(9)
寫評論提問

说明书
  • alpha-Adaptin 1/2 抗体 C-8 是小鼠单克隆 IgG2a κ,α-Adaptin 1/2抗体, 在24篇文献中引用,规格为200 µg/ml
  • 针对氨基酸678-977(缺失707-728)的α-Adaptin 2的mouse起源的
  • alpha-Adaptin 1/2 抗体 (C-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 α-Adaptin 1 and α-Adaptin 2
  • 抗alpha-Adaptin 1/2抗体(C-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取α-Adaptin 1/2 (C-8): sc-17771免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 α-Adaptin 1/2 抗体 (C-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与α-Adaptin 1/2 抗体 (C-8) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

α-Adaptin 1/2抗体(C-8)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶 酶联免疫吸附测定(ELISA)。α-Adaptin 1/2 (C-8) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合型。α-Adaptin在形成包被有衣藻素的囊泡中起着至关重要的作用,而囊泡对于受体介导的内吞作用至关重要,这一过程可使细胞内化营养物质和信号分子。α-Adaptin主要位于质膜上,是AP-2复合物的重要组成部分,负责介导囊泡从质膜上的运输。α-Adaptin与clathrin和其他适配蛋白的相互作用对于clathrin包被小泡的正确组装至关重要,可确保有效的内吞作用和细胞对必需物质的摄取。此外,α-Adaptin的剪接变体存在于不同的组织中,这凸显了α-Adaptin在各种细胞环境中的功能多样性和重要性。通过利用抗α-Adaptin 1/2抗体(C-8),研究人员可以深入了解内吞作用的机制以及α-Adaptin在细胞运输过程中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

α-Adaptin 1/2 抗体 (C-8) 参考文献:

  1. 对α-适配蛋白附属结构域结合多种配体的结构解释。  |  Owen, DJ., et al. 1999. Cell. 97: 805-15. PMID: 10380931
  2. 突触结合蛋白与mu2-和α-adaptin的双重相互作用促进了包被有包涵体的凹坑的形成。  |  Haucke, V., et al. 2000. EMBO J. 19: 6011-9. PMID: 11080148
  3. 在器官培养中,拉伸应力诱导小鼠颅骨中α-适配蛋白C的产生:内吞作用可能参与机械应力刺激的成骨细胞分化。  |  Shimomura, J., et al. 2003. J Cell Physiol. 195: 488-96. PMID: 12704659
  4. 鉴定出一种内吞蛋白家族,该家族定义了新的α-adaptin耳结合基序。  |  Ritter, B., et al. 2003. EMBO Rep. 4: 1089-95. PMID: 14555962
  5. HM1.24通过与alpha-adaptin相互作用,通过Clathrin介导的内吞作用从脂筏内吞。  |  Masuyama, N., et al. 2009. J Biol Chem. 284: 15927-41. PMID: 19359243
  6. Dynamin光致失活可阻断Clathrin和α-adaptin的结合,并诱导大量膜回收。  |  Kasprowicz, J., et al. 2014. J Cell Biol. 204: 1141-56. PMID: 24662566
  7. 控制14-3-3与内吞性适配子Numb结合以及Numb·α-adaptin复合物解离的结构决定因素。  |  Chen, X., et al. 2018. J Biol Chem. 293: 4149-4158. PMID: 29382713
  8. 内部 eGFP 标记的α-adaptin 是一种功能齐全, 经过改进的凝集素包被坑动态受信标记物。  |  Mino, RE., et al. 2020. Traffic. 21: 603-616. PMID: 32657003
  9. α-adaptin异构体的表达和定位。  |  Ball, CL., et al. 1995. J Cell Sci. 108 (Pt 8): 2865-75. PMID: 7593326
  10. α-adaptin的耳与Eps 15蛋白的COOH-末端结构域相互作用。  |  Benmerah, A., et al. 1996. J Biol Chem. 271: 12111-6. PMID: 8662627

订购信息

产品名称产品编号规格价格数量收藏夹

α-Adaptin 1/2 抗体 (C-8)

sc-17771
200 µg/ml
$316.00

α-Adaptin 1/2 (C-8): m-IgG Fc BP-HRP 套装

sc-528302
200 µg Ab; 10 µg BP
$354.00

α-Adaptin 1/2 (C-8): m-IgGκ BP-HRP 套装

sc-520645
200 µg Ab, 40 µg BP
$354.00

α-Adaptin 1/2 抗体 (C-8) AC

sc-17771 AC
500 µg/ml, 25% agarose
$416.00

α-Adaptin 1/2 抗体 (C-8) HRP

sc-17771 HRP
200 µg/ml
$316.00

α-Adaptin 1/2 抗体 (C-8) FITC

sc-17771 FITC
200 µg/ml
$330.00

α-Adaptin 1/2 抗体 (C-8) PE

sc-17771 PE
200 µg/ml
$343.00

α-Adaptin 1/2 抗体 (C-8) Alexa Fluor® 488

sc-17771 AF488
200 µg/ml
$357.00

α-Adaptin 1/2 抗体 (C-8) Alexa Fluor® 546

sc-17771 AF546
200 µg/ml
$357.00

α-Adaptin 1/2 抗体 (C-8) Alexa Fluor® 594

sc-17771 AF594
200 µg/ml
$357.00

α-Adaptin 1/2 抗体 (C-8) Alexa Fluor® 647

sc-17771 AF647
200 µg/ml
$357.00

α-Adaptin 1/2 抗体 (C-8) Alexa Fluor® 680

sc-17771 AF680
200 µg/ml
$357.00

α-Adaptin 1/2 抗体 (C-8) Alexa Fluor® 790

sc-17771 AF790
200 µg/ml
$357.00

Can α-Adaptin 1/2 (C-8): sc-17771 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, α-Adaptin 1/2 (C-8): sc-17771 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-02-07
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_17771, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 119ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good AB for WBI used this AB for WesternBlots with MCF7 whole cell lysate : strong signal detected by ECL.
Date published: 2020-07-31
Rated 5 out of 5 by from WB for brain tissueGood for WB, with low background. Does not work for IF or ICC in rat neuronal cultures.
Date published: 2020-01-07
Rated 5 out of 5 by from Great antibody for WBGreat antibody for western blot using this antibody for detect CASK in mouse brain lysate.
Date published: 2017-03-23
Rated 5 out of 5 by from Very popular choice!The (C-8) clone for alpha-Adaptin 1/2 is a well-established antibody with over a dozen known publications citing use of this clone.
Date published: 2017-01-21
Rated 5 out of 5 by from Strong band in WB in HepG2Strong band in WB in HepG2, KNRK and NIH/3T3 whole cell lysates and mouse liver tissue extract. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from no commentno comment
Date published: 2015-04-29
Rated 5 out of 5 by from Good specificity in WB in HepG2 whole cell lysateGood specificity in WB in HepG2 whole cell lysate. -SCBT QC
Date published: 2015-04-27
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_17771, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
α-Adaptin 1/2 抗体 (C-8) is rated 4.9 out of 5 by 9.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_9
  • loc_zh_CN, sid_17771, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 103ms
  • REVIEWS, PRODUCT