Date published: 2025-9-6

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ALG-2 抗体 (H-11): sc-376950

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说明书
  • ALG-2 抗体 H-11 是小鼠单克隆 IgG1 κ,ALG-2抗体, 在1篇文献中引用,规格为200 µg/ml
  • 免疫human物种的代表全长ALG-2的氨基酸1-191
  • ALG-2 抗体 (H-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 ALG-2
  • 抗ALG-2抗体(H-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ALG-2 (H-11): sc-376950免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是ALG-2 Antibody (H-11) for WB applications. 的首选辅助检测试剂,这些试剂现与ALG-2 Antibody (H-11) 打包提供(请参阅下面的订购信息)。

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関連項目

ALG-2抗体(H-11)是一种IgG1 κ小鼠单克隆ALG-2抗体(也称为ALG-2抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的ALG-2蛋白。ALG-2抗体(H-11)既有非缀合的抗ALG-2抗体形式,也有多种缀合的抗ALG-2抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®缀合物。细胞内Ca2+浓度的增加会诱导细胞凋亡。糖皮质激素介导和T细胞受体介导的途径需要短暂升高的Ca2+浓度,从而导致T细胞凋亡。ALG-2(凋亡相关基因2)是一种Ca2+结合蛋白,参与凋亡程序后期发生的调节事件,其中几个死亡信号会汇聚在一起。ALG-2是一种在正常脑中表达的蛋白质,在缺血性脑中的表达量更大。ALG-2蛋白含有五个EF手样基序,与五EF手家族成员具有同源性,其中包括钙蛋白酶小亚基sorcin和Grancalcin。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ALG-2 抗体 (H-11) 参考文献:

  1. 暂时性局灶性脑缺血后大鼠大脑中与细胞凋亡相关的基因 2 (ALG2) 的表达增加。  |  Li, W., et al. 2000. Neuroscience. 96: 161-8. PMID: 10683420
  2. 两种形式的凋亡相关蛋白 ALG-2 具有不同的 Ca(2+) 亲和力和目标识别能力。  |  Tarabykina, S., et al. 2000. J Biol Chem. 275: 10514-8. PMID: 10744743
  3. Ca2+ 在细胞杀伤中的作用  |  Nicotera, P., et al. 1990. Chem Res Toxicol. 3: 484-94. PMID: 2103319
  4. 通过 CD3/T 细胞受体复合物的刺激对未成熟胸腺细胞的钙依赖性杀伤。  |  McConkey, DJ., et al. 1989. J Immunol. 143: 1801-6. PMID: 2528581
  5. 糖皮质激素通过提高细胞膜 Ca2+ 浓度激活胸腺细胞的自杀过程。  |  McConkey, DJ., et al. 1989. Arch Biochem Biophys. 269: 365-70. PMID: 2537063
  6. 干扰细胞凋亡:钙(2+)结合蛋白 ALG-2 和阿尔茨海默病基因 ALG-3。  |  Vito, P., et al. 1996. Science. 271: 521-5. PMID: 8560270
  7. 参与 T 细胞受体诱导的程序性细胞死亡的基因功能克隆。  |  D'Adamio, L., et al. 1997. Semin Immunol. 9: 17-23. PMID: 9106304
  8. 具有五个 EF-手基序的 Ca2+ 结合蛋白家族不断壮大。  |  Maki, M., et al. 1997. Biochem J. 328 (Pt 2): 718-20. PMID: 9441591
  9. 与细胞凋亡相关的基因 ALG-2 mRNA 在幼鼠和老龄鼠脑中的定位  |  Venn, MK. and Conway, EL. 1998. Neuroreport. 9: 1981-5. PMID: 9674578

订购信息

产品名称产品编号规格价格数量收藏夹

ALG-2 抗体 (H-11)

sc-376950
200 µg/ml
$316.00

ALG-2 (H-11): m-IgG Fc BP-HRP 套装

sc-527557
200 µg Ab; 10 µg BP
$354.00

ALG-2 (H-11): m-IgG1 BP-HRP 套装

sc-532930
200 µg Ab; 20 µg BP
$354.00

ALG-2 抗体 (H-11) AC

sc-376950 AC
500 µg/ml, 25% agarose
$416.00

ALG-2 抗体 (H-11) HRP

sc-376950 HRP
200 µg/ml
$316.00

ALG-2 抗体 (H-11) FITC

sc-376950 FITC
200 µg/ml
$330.00

ALG-2 抗体 (H-11) PE

sc-376950 PE
200 µg/ml
$343.00

ALG-2 抗体 (H-11) Alexa Fluor® 488

sc-376950 AF488
200 µg/ml
$357.00

ALG-2 抗体 (H-11) Alexa Fluor® 546

sc-376950 AF546
200 µg/ml
$357.00

ALG-2 抗体 (H-11) Alexa Fluor® 594

sc-376950 AF594
200 µg/ml
$357.00

ALG-2 抗体 (H-11) Alexa Fluor® 647

sc-376950 AF647
200 µg/ml
$357.00

ALG-2 抗体 (H-11) Alexa Fluor® 680

sc-376950 AF680
200 µg/ml
$357.00

ALG-2 抗体 (H-11) Alexa Fluor® 790

sc-376950 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with ALG-2 (H-11): sc-376950 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from IF Sections!I used this AB (1:1000) in chick developing spinal cord sections and it looks great! When compared to in situs of ALG-2, it is quite similar with an enrichment in the motor column. Clean and consistent staining.
Date published: 2018-03-13
Rated 5 out of 5 by from Works great in Western blotI tried this antibody with the dilution of 1:1000 in milk. Results are perfect.
Date published: 2017-09-11
Rated 5 out of 5 by from The antibody works well for western blot.I applied a free sample of the antibody and detect the ALG-2 in HEK293T cells. The band is very clear and specific.
Date published: 2017-04-11
Rated 5 out of 5 by from Produced beautiful immunofluorescence cytoplasmicProduced beautiful immunofluorescence cytoplasmic and nuclear staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-08
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ALG-2 抗体 (H-11) is rated 4.8 out of 5 by 5.
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