Akt3抗体 (EE-M14) 推荐用于 mouse, rat 和 human 来源的Akt3 WB, IP 和 ELISA检测

Akt3 抗体 (EE-M14): sc-134254

Akt3抗体 (EE-M14) is rated 4.6 out of 5 by 9.
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说明书

    • Akt3抗体(EE-M14)是小鼠单克隆IgG2a κ, 在4篇文献中引用,规格为100 µg/ml
    • raised against recombinant Akt3 protein of human origin
    • 推荐用于 mouse, rat 和 human 来源的Akt3 WB, IP 和 ELISA检测
    • 关于如何获取Akt3 (EE-M14): sc-134254免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • 目前,我们还没有完成Akt3 抗体 (EE-M14)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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For Western Blot, is it recommended to use denatured or non-denatured conditions with Akt3 (EE-M14): sc-134254 antibody?

Asked by: Randy McDonald
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 4 out of 5 by from Good for WB I've tested this antibody in western blotting and it worked well at 1:1000 dilution.
Date published: 2021-01-28
Rated 4 out of 5 by from Worked for WB Gave a good band after longer exposure time. Used 1:200 for mouse tissue lysates
Date published: 2018-03-23
Rated 4 out of 5 by from Good antibody Worked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from a little weak this antibody is specific , but the band is a little weak.
Date published: 2017-07-07
Rated 5 out of 5 by from Works well for Western with HSC-3 cells WB: 1:200. Single strong band with HSC-3 and cal-27 cells.
Date published: 2017-01-10
Rated 5 out of 5 by from Single strong band in mouse NIH/3T3 samples Single strong band in mouse NIH/3T3 samples. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Works well for Western in MCF7 cells Works well for Western in MCF7 cells. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Great WB data in HeLa cells Great WB data in HeLa cells. -SCBT QC
Date published: 2015-02-06
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