Ajuba Antibody (E-3): sc-374610

Ajuba Antibody (E-3) is a mouse monoclonal IgG2b antibody that detects Ajuba in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ajuba is a crucial LIM protein that plays a significant role in various cellular processes, particularly in the regulation of the IL-1 signaling pathway, where Ajuba modulates interleukin-1-induced NFkB activation by influencing the assembly and activity of the αPKC/p62/TRAF6 multiprotein signaling complex. This regulation is vital for controlling inflammatory responses and cellular signaling, which can have implications in various diseases, including cancer and autoimmune disorders. Additionally, Ajuba is involved in cadherin-mediated cell-cell adhesion and influences cell migration by regulating the synthesis of phosphatidylinositol 4,5-bisphosphate (PIP2) through direct activation of PIPKIα activity. In differentiating mouse embryonic stem cells, Ajuba transcription is notably elevated, indicating its importance in developmental processes. In adult mouse tissues, Ajuba is expressed in the skin, brain, and genitourinary organs, highlighting its diverse functional roles. Immunofluorescence (IF) analysis of unsynchronized HeLa cells reveals cytoplasmic localization, while in cells synchronized at the G2/M phase, Ajuba localizes to the centrosome, where Ajuba interacts with LATS2 to regulate spindle apparatus organization through the recruitment of γ-tubulin, underscoring its critical role in cell division and maintaining genomic stability.

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Ajuba Antibody (E-3) References:

1. Ajuba overexpression regulates mitochondrial potential and glucose uptake through YAP/Bcl-xL/GLUT1 in human gastric cancer.  |  Li, H., et al. 2019. Gene. 693: 16-24. PMID: 30690182
2. The LIM Protein Ajuba Augments Tumor Metastasis in Colon Cancer.  |  Dommann, N., et al. 2020. Cancers (Basel). 12: PMID: 32679899
3. Ajuba transactivates N-cadherin expression in colorectal cancer cells through interaction with Twist.  |  Wu, Z., et al. 2021. J Cell Mol Med. 25: 8006-8014. PMID: 34173718
4. miR-433-3p Targets AJUBA to Inhibit Malignant Progression of Glioma.  |  Zhang, J., et al. 2022. Neuroimmunomodulation. 29: 44-54. PMID: 34518486
5. The SQSTM1/p62 UBA domain regulates Ajuba localisation, degradation and NF-κB signalling function.  |  Sultana, MA., et al. 2021. PLoS One. 16: e0259556. PMID: 34735553
6. Ajuba Overexpression Promotes Breast Cancer Chemoresistance and Glucose Uptake through TAZ-GLUT3/Survivin Pathway.  |  Li, X., et al. 2022. Biomed Res Int. 2022: 3321409. PMID: 35178446
7. AJUBA and WTIP can compete with LIMD1 for junctional localization and LATS regulation.  |  Kirichenko, E. and Irvine, KD. 2022. MicroPubl Biol. 2022: PMID: 36439396
8. Activation of Hippo Pathway Damages Slit Diaphragm by Deprivation of Ajuba Proteins.  |  Gilhaus, K., et al. 2023. J Am Soc Nephrol. 34: 1039-1055. PMID: 36930055
9. LIM domain-containing protein Ajuba inhibits chemotherapy-induced apoptosis by negatively regulating p53 stability in colorectal cancer cells.  |  Xu, B., et al. 2023. Mol Oncol. 17: 1678-1691. PMID: 36931700
10. Transcription factor EHF interacting with coactivator AJUBA aggravates malignancy and acts as a therapeutic target for gastroesophageal adenocarcinoma.  |  Peng, L., et al. 2024. Acta Pharm Sin B. 14: 2119-2136. PMID: 38799645

The preferred Ajuba antibody is Ajuba Antibody (B-3): sc-398008.