Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
aggrecan Antibody (4F4) - Immunohistochemistry - Image 382986
aggrecan 抗体 (4F4): sc-33695. 对福尔马林固定, 石蜡包埋的人软骨组织进行免疫过氧化物酶染色, 显示软骨细胞的胞浆染色.
aggrecan Antibody (4F4) - Immunohistochemistry - Image 382986
aggrecan 抗体 (4F4): sc-33695. 对福尔马林固定, 石蜡包埋的人软骨组织进行免疫过氧化物酶染色, 显示软骨细胞的胞浆染色.
aggrecan Antibody (4F4) - Western Blotting - Image 11510
aggrecan 抗体 (4F4): sc-33695. 用软骨素酶 ABC, 角蛋白酶 I 和内-β-半乳糖苷酶预消化的人关节软骨中 aggrecan 表达的 Western 印迹分析. 意大利帕尔马大学 Roberto Perris 博士友情提供.
aggrecan Antibody (4F4) - Immunohistochemistry - Image 37977
aggrecan 抗体 (4F4): sc-33695. 福尔马林固定, 石蜡包埋的人体软组织的免疫过氧化物酶染色, 低倍 (A) 和高倍 (B) 放大后显示软骨细胞的细胞质染色. 瑞典人类蛋白质图谱 (HPA) 计划友情提供.
aggrecan 抗体 (4F4): sc-33695. 对福尔马林固定, 石蜡包埋的人软骨组织进行免疫过氧化物酶染色, 显示软骨细胞的胞浆染色.

aggrecan 抗体 (4F4): sc-33695

4.5(13)
寫評論提問

说明书
  • aggrecan 抗体 4F4 是小鼠单克隆 IgG1 κ，aggrecan抗体, 在40篇文献中引用，规格为200 µg/ml
  • 针对human关节软骨aggrecan
  • aggrecan 抗体 (4F4) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 aggrecan
  • 抗aggrecan抗体（4F4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取aggrecan (4F4): sc-33695的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 aggrecan 抗体 (4F4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与aggrecan 抗体 (4F4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

aggrecan 抗体（4F4）是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测人类来源的 aggrecan 蛋白。抗 aggrecan 抗体（4F4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白 素（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。 蛋白是一种大型硫酸软骨素蛋白多糖，在维持软骨的结构完整性和弹性方面起着至关重要的作用。 蛋白主要位于软骨的细胞外基质中，与透明质酸和连接蛋白形成聚集物，有助于提高组织的抗压强度和承受机械应力的能力。这种定位对于软骨的正常功能至关重要，因为聚糖与胶原纤维相互作用，为关节提供必要的支撑和缓冲。值得注意的是，聚糖降解通常与各种形式的炎症性关节炎有关，导致软骨损伤和丢失，这凸显了在临床环境中监测聚糖水平的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

aggrecan 抗体 (4F4) 参考文献:

  1. C 端 G3 结构域在 aggrecan 核心蛋白的分拣和分泌以及累积突变前体的泛素介导降解中的作用。  |  Domowicz, MS., et al. 2000. J Biol Chem. 275: 35098-105. PMID: 11063750
  2. 本应通过外排途径运输的 Aggrecan 结构域被误导至细胞核。  |  Chen, TL., et al. 2001. Exp Cell Res. 263: 224-35. PMID: 11161721
  3. aggrecan 结构域在生物合成, 糖胺聚糖修饰和产品分泌中的作用。  |  Kiani, C., et al. 2001. Biochem J. 354: 199-207. PMID: 11171095
  4. Aggrecan：自身免疫反应的目标分子  |  Buzás, EI., et al. 1996. Pathol Oncol Res. 2: 219-228. PMID: 11173607
  5. 软骨蛋白多糖。  |  Knudson, CB. and Knudson, W. 2001. Semin Cell Dev Biol. 12: 69-78. PMID: 11292372
  6. 基于 Aggrecan 的细胞外基质是人类基底神经节回路不可或缺的一部分。  |  Brückner, G., et al. 2008. Neuroscience. 151: 489-504. PMID: 18055126
  7. 通过组织自溶从软骨中提取凝集素肽。  |  Nakano, T., et al. 2014. Recent Pat Food Nutr Agric. 6: 54-9. PMID: 24923587
  8. Aggrecan 引导细胞外基质介导的神经元可塑性  |  Rowlands, D., et al. 2018. J Neurosci. 38: 10102-10113. PMID: 30282728
  9. 血管生成素在心血管发育和疾病中的作用。  |  Koch, CD., et al. 2020. J Histochem Cytochem. 68: 777-795. PMID: 32870742
  10. Aggrecan：研究生物物理和生物力学特性的方法。  |  Nia, HT., et al. 2022. Methods Mol Biol. 2303: 209-226. PMID: 34626381
  11. FMRP 对 aggrecan mRNA 翻译的调控控制着神经元周围网的发育。  |  Van't Spijker, HM. and Richter, JD. 2024. J Neurochem. 168: 1909-1922. PMID: 38225196
  12. 受外周能量状态调控的可塑性凝集素屏障可控制代谢信号进入弓状神经元。  |  Kuczynski-Noyau, L., et al. 2024. Nat Commun. 15: 6701. PMID: 39112471

订购信息

产品名称产品编号规格价格数量收藏夹

aggrecan 抗体 (4F4)

sc-33695
200 µg/ml
$316.00

aggrecan (4F4): m-IgG Fc BP-HRP 套装

sc-526707
200 µg Ab; 10 µg BP
$354.00

aggrecan (4F4): m-IgG1 BP-HRP 套装

sc-532080
200 µg Ab; 20 µg BP
$354.00

aggrecan 抗体 (4F4) AC

sc-33695 AC
500 µg/ml, 25% agarose
$416.00

aggrecan 抗体 (4F4) HRP

sc-33695 HRP
200 µg/ml
$316.00

aggrecan 抗体 (4F4) FITC

sc-33695 FITC
200 µg/ml
$330.00

aggrecan 抗体 (4F4) PE

sc-33695 PE
200 µg/ml
$343.00

aggrecan 抗体 (4F4) Alexa Fluor® 488

sc-33695 AF488
200 µg/ml
$357.00

aggrecan 抗体 (4F4) Alexa Fluor® 546

sc-33695 AF546
200 µg/ml
$357.00

aggrecan 抗体 (4F4) Alexa Fluor® 594

sc-33695 AF594
200 µg/ml
$357.00

aggrecan 抗体 (4F4) Alexa Fluor® 647

sc-33695 AF647
200 µg/ml
$357.00

aggrecan 抗体 (4F4) Alexa Fluor® 680

sc-33695 AF680
200 µg/ml
$357.00

aggrecan 抗体 (4F4) Alexa Fluor® 790

sc-33695 AF790
200 µg/ml
$357.00

About your Aggrecan antibody.sc-33695, according to the datasheet, it is indicated to be reactive with human samples. I would like to use this antibody for immunostaining purposes,is it suitable for use with rat or mouse tissues?

Asked by: Anonymous
Thank you for your question. sc-33695 is for human samples only, mice and rats are not supported. Thank you.
Answered by: Jiajun Z
Date published: 2025-08-07

Hi there! Has this antibody been used in brain/spinal cord tissue for IHC?

Asked by: shantan
Thank you for your question. We have many citations of this antibody in IF and IHC, but unfortunately I do not see if any of them are in brain/spinal cord. Aggrecan is detected in chondrocytes throughout the developing skeleton/ cartilage and we have many publications in bone marrow.
Answered by: Tech Service
Date published: 2019-11-19

Can aggrecan (4F4) sc-33695 monoclonal antibody be used with porcine tissue or cells?

Asked by: Vanessa_silos
Thank you for your question. We have not tested this antibody in porcine tissue. We are aware of one product citation using this antibody in porcine tissue. Please refer to the following publication for details and verification, PMID: 19112334.
Answered by: Technical Support
Date published: 2019-04-13

I would like to detection of aggrecan in western blot assay. I tried dilution 1:500 in 5% skinmilk. and 2nd antibody used sc-2005 product. but It does not detection. How can I do? my sample orign is human pellet.

Asked by: vtml
Thank you for your question. We recommend starting with a 1:100 primary antibody dilution for Western blot. For further assistance, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-06

Can aggrecan (4F4): sc-33695 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, aggrecan (4F4): sc-33695 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2019-02-28
  • y_2025, m_8, d_27, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_5
  • loc_zh_CN, sid_33695, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 105ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good, it can be used in IHCI used in bone in 1:100. it's specific. I think it can used in 1:200.
Date published: 2017-08-09
Rated 4 out of 5 by from Good antibody in detecting aggrecanThis antibody works very well in detecting cell cycle protein aggrecan without any nonspecific bands.
Date published: 2017-06-01
Rated 5 out of 5 by from Great antibody.Antibody provides clear and specific results.Great antibody.
Date published: 2017-02-13
Rated 5 out of 5 by from Excellentaggrecan antibody antibody is really great. we got specific signal for the protein at the right molecular weight.
Date published: 2017-02-08
Rated 5 out of 5 by from The use of this antibody was satisfactoryThe use of this antibody has a good specificity for WB dilution and the 1:500 results are perfect.
Date published: 2017-02-05
Rated 5 out of 5 by from Good, versatile antibodyThe aggrecan Antibody (4F4): sc-33695 works well for multiple applications including WB, IP, IF and IHC(P). This antibody has also been cited several times for further verification.
Date published: 2017-01-20
Rated 5 out of 5 by from Performance good antibodyThis antibody in the use of performance is very good, it produces a good effect in mice.
Date published: 2016-12-21
Rated 5 out of 5 by from Fantastic antibody of Agg!I have used many kinds of Agg from different companies, but unfortunately, some of them are not work well... But this Agg can easily get strong band at 1:1000! Beyond my imagination!
Date published: 2016-12-19
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_33695, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 23ms
  • REVIEWS, PRODUCT
aggrecan 抗体 (4F4) is rated 4.5 out of 5 by 13.
  • y_2025, m_8, d_27, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_13
  • loc_zh_CN, sid_33695, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 120ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。