acrogranin Double Nickase Plasmid (h): sc-401924-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- acrogranin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- acrogranin Double Nickase Plasmid (h) and acrogranin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GRN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: acrogranin Antibody (C-11): sc-377036
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
acrogranin Double Nickase Plasmid (h) | sc-401924-NIC | 20 µg | $410.00 | |||
acrogranin Double Nickase Plasmid (h2) | sc-401924-NIC-2 | 20 µg | $410.00 |
Human GRN encodes acrogranin (progranulin), a secreted glycoprotein processed into granulins that regulate cell survival, motility, and tissue remodeling. Acrogranin influences inflammatory signaling and lysosomal homeostasis, with reported links to TNF/NF-κB responses, growth factor–associated pathways, and regulation of extracellular matrix dynamics. GRN-dependent trafficking and proteolysis intersect with endo-lysosomal function and microglial biology, making GRN a key node in neuroinflammation and proteostasis. Genetic and functional perturbations of GRN are widely studied in neurodegeneration and inflammatory disease models, where altered progranulin levels affect neuronal resilience and immune cell activation states.
acrogranin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GRN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GRN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GRN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GRN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.