acrogranin CRISPR/Cas9 KO Plasmid (m): sc-420696
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- acrogranin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the acrogranin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
acrogranin CRISPR/Cas9 KO Plasmid (m) | sc-420696 | 20 µg | $397.00 |
Overview
Grn encodes acrogranin (progranulin), a secreted and lysosome-associated glycoprotein that modulates cell growth, survival, and inflammatory signaling in diverse tissues. In mouse, acrogranin participates in endolysosomal homeostasis and proteostasis and can influence pathways linked to microglial activation, cytokine networks, and extracellular matrix remodeling. Altered GRN/acrogranin function has been connected to neurodegeneration, aberrant immune responses, and tissue repair phenotypes, making it a useful node for studying neuroinflammation and lysosomal stress biology. Grn-dependent processes are also relevant to models of metabolic and fibrotic remodeling where secreted growth factors and lysosomal function intersect.
acrogranin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Grn gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Grn together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Grn open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish acrogranin protein expression.
This CRISPR knockout system enables efficient generation of Grn-deficient cell models for investigation of acrogranin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Grn exon(s) critical for acrogranin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Grn genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by acrogranin CRISPR/Cas9 KO Plasmid (m) and acrogranin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Grn locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by acrogranin HDR Plasmid (m) and acrogranin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Grn homology arms to support homology-directed repair at defined Grn target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.