Date published: 2025-9-7
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ACCα Antibody (D-5) - Western Blotting - Image 58513
ACCα (D-5): sc-137104. DU 145 (A) 和 Jurkat (B) 全细胞裂解液中 ACCα 表达的 Western 印迹分析.
ACCα Antibody (D-5) - Western Blotting - Image 58513
ACCα (D-5): sc-137104. DU 145 (A) 和 Jurkat (B) 全细胞裂解液中 ACCα 表达的 Western 印迹分析.
ACCα (D-5): sc-137104. DU 145 (A) 和 Jurkat (B) 全细胞裂解液中 ACCα 表达的 Western 印迹分析.

ACCα 抗体 (D-5): sc-137104

4.1(15)
寫評論提問

说明书
  • ACC alpha 抗体 D-5 是小鼠单克隆 IgG2b κ，ACCα抗体, 在30篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-76，在ACCα的human起源的N-terminus处进行映射
  • ACC alpha 抗体 (D-5) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 ACCα
  • 抗ACC alpha抗体（D-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ACCα (D-5): sc-137104的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是ACCα Antibody (D-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与ACCα Antibody (D-5) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外ACC抗体，包括ACCβ

ACCα抗体（D-5）是一种IgG2b κ小鼠单克隆ACC alpha抗体（也称为ACACA抗体、乙酰辅酶A羧化酶Alpha抗体、乙酰辅酶A羧化酶1抗体、ACC1抗体、ACC-Alpha抗体、ACAC抗体或ACCA抗体），可通过WB、IP、IF和ELISA检测人源的ACC alpha蛋白。ACCα抗体（D-5）既有非偶联抗ACC alpha抗体形式，也有多种偶联形式的抗ACC alpha抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。乙酰辅酶A羧化酶（ACC）是一个复杂的多功能酶系统，可催化乙酰辅酶A羧化成丙二酰辅酶A，这是脂肪酸合成中的限速步骤。运动可降低人体肌肉中乙酰辅酶A羧化酶的活性。ACCα（ACC1）是长链脂肪酸生物合成中的限速酶，而ACCβ（ACC2）可控制线粒体脂肪酸氧化。ACC的这两种异构体控制细胞中的脂肪酸含量。ACCα的催化功能受靶向丝氨酸残基的磷酸化（失活）和去磷酸化（激活）以及柠檬酸或棕榈酰辅酶A的别构转化的调节，这是该酶的短期调节机制。编码ACCα的基因定位于人类17号染色体，并编码一种ACC形式，这是脂肪生成组织中的主要ACC。ACCβ的催化核心与ACCα的同源，区别在于其在N端有一个额外的约150个氨基酸的肽段。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ACCα 抗体 (D-5) 参考文献:

  1. 乙酰-CoA羧化酶 2 的亚细胞定位。  |  Abu-Elheiga, L., et al. 2000. Proc Natl Acad Sci U S A. 97: 1444-9. PMID: 10677481
  2. 运动会降低人体肌肉中乙酰-CoA 羧化酶的活性。  |  Dean, D., et al. 2000. Diabetes. 49: 1295-300. PMID: 10923628
  3. 克隆人乙酰-CoA羧化酶 beta 启动子及其受肌肉调节因子的调控。  |  Lee, JJ., et al. 2001. J Biol Chem. 276: 2576-85. PMID: 11076940
  4. 胰岛β细胞中谷氨酸和镁敏感蛋白磷酸酶对乙酰-CoA羧化酶的激活作用  |  Kowluru, A., et al. 2001. Diabetes. 50: 1580-7. PMID: 11423479
  5. 乙酰辅酶A羧化酶α基因与乳腺癌易感性。  |  Sinilnikova, OM., et al. 2004. Carcinogenesis. 25: 2417-24. PMID: 15333468
  6. RNA干扰介导的乙酰辅酶A羧化酶α基因沉默诱导前列腺癌细胞的生长抑制和凋亡。  |  Brusselmans, K., et al. 2005. Cancer Res. 65: 6719-25. PMID: 16061653
  7. 乙酰-CoA羧化酶α对乳腺癌细胞的存活至关重要。  |  Chajès, V., et al. 2006. Cancer Res. 66: 5287-94. PMID: 16707454
  8. 乙酰-CoA羧化酶-α抑制剂 TOFA 可诱导人类癌细胞凋亡。  |  Wang, C., et al. 2009. Biochem Biophys Res Commun. 385: 302-6. PMID: 19450551
  9. ACACA（乙酰辅酶A羧化酶α）通过成纤维细胞衰老表型转换参与乳腺癌肺转移前微环境形成。  |  Huang, YC., et al. 2023. Cell Oncol (Dordr). 46: 643-660. PMID: 36607556
  10. 乙酰辅酶A羧化酶α作为肝细胞癌的预后和靶向候选物的鉴定。  |  Shen, Y., et al. 2023. Clin Transl Oncol. 25: 2499-2513. PMID: 36976490
  11. 烟酸姜黄素通过抑制AKR1B10/ACCα介导的甘油三酯合成来改善NASH。  |  Lin, XL., et al. 2024. Lipids Health Dis. 23: 201. PMID: 38937844
  12. 哺乳动物乙酰辅酶 A 羧化酶的调控。  |  Kim, KH. 1997. Annu Rev Nutr. 17: 77-99. PMID: 9240920

订购信息

产品名称产品编号规格价格数量收藏夹

ACCα 抗体 (D-5)

sc-137104
200 µg/ml
$316.00

ACCα (D-5): m-IgGκ BP-HRP 套装

sc-521427
200 µg Ab, 40 µg BP
$354.00

ACCα (D-5): m-IgG2b BP-HRP 套装

sc-548895
200 µg Ab; 10 µg BP
$354.00

ACCα 抗体 (D-5) AC

sc-137104 AC
500 µg/ml, 25% agarose
$416.00

ACCα 抗体 (D-5) HRP

sc-137104 HRP
200 µg/ml
$316.00

ACCα 抗体 (D-5) FITC

sc-137104 FITC
200 µg/ml
$330.00

ACCα 抗体 (D-5) PE

sc-137104 PE
200 µg/ml
$343.00

ACCα 抗体 (D-5) Alexa Fluor® 488

sc-137104 AF488
200 µg/ml
$357.00

ACCα 抗体 (D-5) Alexa Fluor® 546

sc-137104 AF546
200 µg/ml
$357.00

ACCα 抗体 (D-5) Alexa Fluor® 594

sc-137104 AF594
200 µg/ml
$357.00

ACCα 抗体 (D-5) Alexa Fluor® 647

sc-137104 AF647
200 µg/ml
$357.00

ACCα 抗体 (D-5) Alexa Fluor® 680

sc-137104 AF680
200 µg/ml
$357.00

ACCα 抗体 (D-5) Alexa Fluor® 790

sc-137104 AF790
200 µg/ml
$357.00

请问AACα的分子量是多少呢？跟ACCβ不一样，是吧

Asked by: 我对上帝笑
感谢您的提问。预测的Molecular Weight of ACCα: 265 kDa，Molecular Weight of ACCβ: 275-280 kDa。关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. Predicted Molecular Weight of ACCα: 265 kDa, Molecular Weight of ACCβ: 275-280 kDa. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-11

What blocking solution should I use with ACCα (D-5): sc-137104 monoclonal antibody for Western blot?

Asked by: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support 3
Date published: 2017-02-09
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Rated 1 out of 5 by from L'anticorpo non riconosce la proteina target.Ho avuto modo di testare questo anticorpo in western blotting, su estratti proteici di cellule Ho avuto modo di testare questo anticorpo in western blotting, su estratti proteici derivanti da cellule RD (50 ug di estratto proteico), ottenendo scarsi risultati. Nelle condizioni sperimentali utilizzate, l'anticorpo non ha riconosciuto la proteina target e sono state rilevate delle bande aspecifiche, con un MW non atteso.
Date published: 2023-11-07
Rated 3 out of 5 by from Moderate results with Huh7 cell lysatesTested on Western blot on whole cell lysate (Huh7 cell line). The antibody is specific for ACCα, but the signal is not very strong and the background is a little bit high. Application: Western blot Sample: whole lysate of human hepatocytes from Huh7 cell line Gel running conditions: reduced, denaturing, 4-12% SDS-PAGE Loading amount: 30 µg protein/lane Transfer: semi-dry, on nitrocellulose membrane (0.45 µm) Blocking agent: 5% milk in TBST buffer, for 1h, RT Primary antibody dilution- 1: 400 in 5% milk, for 16 h at 4°C Secondary antibody: non- Santa Cruz antibody anti-mouse-HRP, 1:10000 dilution in 5% milk, for 1h, RT Detection: ECL substrate (femto sensitivity) Exposure time: 20 sec Bands: specific, at 265 kDa; non-specific bands are absent.
Date published: 2023-08-04
Rated 1 out of 5 by from No bandI could not detect the band in Huh1 and HEK293 cells.
Date published: 2022-05-18
Rated 4 out of 5 by from goodThis ab works well for wb in hepatic tissues at 1:800. The result awaits to be shown for HepG2 cells.
Date published: 2017-08-22
Rated 5 out of 5 by from okthe antibody was diluted at the concetration about 1:500 the data are good
Date published: 2017-08-07
Rated 4 out of 5 by from Great featuresI bought this a week ago, I did Western Blot(dilution 1:100), but the baackground is high
Date published: 2017-07-18
Rated 5 out of 5 by from worked greatThis antibody was used in WB(1:1000 dilution) experiments, clean specific bands
Date published: 2017-05-13
Rated 5 out of 5 by from dilution 1:200Working with this antibody, use the dilution 1:200 with a precise position effect and very powerful signal
Date published: 2017-04-22
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ACCα 抗体 (D-5) is rated 4.1 out of 5 by 15.
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