Date published: 2025-9-6

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ACADS 抗体 (G-10): sc-365953

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说明书
  • ACADS 抗体 G-10 是小鼠单克隆 IgG2b κ,ACADS抗体, 在6篇文献中引用,规格为200 µg/ml
  • 针对human起源的ACADS内部区域内的氨基酸104-244进行
  • ACADS 抗体 (G-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 ACADS
  • 抗ACADS抗体(G-10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ACADS (G-10): sc-365953免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRPm-IgGκ BP-HRP是ACADS Antibody (G-10) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与ACADS Antibody (G-10) 打包提供(请参阅下面的订购信息)。

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関連項目

ACADS抗体(G-10)是一种IgG2b κ小鼠单克隆ACADS抗体(也称为ACADS抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的ACADS蛋白。ACADS抗体(G-10)既可作为非偶联的抗ACADS抗体形式,也可作为多种偶联形式的抗ACADS抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。 ACADS(酰基辅酶A脱氢酶,C-2至C-3短链),也称为SCAD或ACAD3,是一种412个氨基酸同源四聚体线粒体黄素蛋白,属于酰基辅酶A脱氢酶家族。 ACADS催化线粒体脂肪酸β-氧化途径的限速步骤。 ACADS突变与脂肪酸氧化缺陷和代谢性疾病有关,如短链酰基辅酶A脱氢酶缺乏症(SCAD缺乏症),一种常染色体隐性遗传性疾病,导致婴儿急性酸中毒和肌肉无力,成人脂质储存性肌病。 SCADS导致血液和尿液中丁酰肉碱和乙基丙二酸积聚。 ACADS含有四个FAD结构域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ACADS 抗体 (G-10) 参考文献:

  1. 短链脂肪酸β-氧化不足会影响睡眠时的θ振荡。  |  Tafti, M., et al. 2003. Nat Genet. 34: 320-5. PMID: 12796782
  2. 中链酰基-CoA 脱氢酶和异(3)戊酰-CoA 脱氢酶的热分解:基因缺陷对酶稳定性影响的研究。  |  Nasser, I., et al. 2004. Biochim Biophys Acta. 1690: 22-32. PMID: 15337167
  3. 人类酰基-CoA脱氢酶-9在不饱和脂肪酸的线粒体β-氧化过程中发挥着新的作用。  |  Ensenauer, R., et al. 2005. J Biol Chem. 280: 32309-16. PMID: 16020546
  4. 硝基烷氧化酶的晶体结构:从黄酶在周转过程中被困的共价复合物洞察反应机理。  |  Nagpal, A., et al. 2006. Biochemistry. 45: 1138-50. PMID: 16430210
  5. 短链酰基辅酶 A 脱氢酶缺乏症的临床, 生化和遗传异质性。  |  van Maldegem, BT., et al. 2006. JAMA. 296: 943-52. PMID: 16926354
  6. 短链酰基-CoA 脱氢酶基因突变(c.319C>T)具有临床异质性,是阿什肯纳兹犹太血统个体的候选创始人突变。  |  Tein, I., et al. 2008. Mol Genet Metab. 93: 179-89. PMID: 18054510
  7. 底物脂肪酰基链特异性的结构基础:人类超长链酰基-CoA 脱氢酶的晶体结构。  |  McAndrew, RP., et al. 2008. J Biol Chem. 283: 9435-43. PMID: 18227065
  8. 罗格列酮对脂肪组织中酰基-CoA 脱氢酶的调节。  |  Goetzman, ES. 2009. Obesity (Silver Spring). 17: 196-8. PMID: 18948967
  9. 新生儿短链酰基辅酶A脱氢酶缺乏症ACADS基因的复合杂合突变:病例报告和文献综述。  |  An, SJ., et al. 2016. Korean J Pediatr. 59: S45-S48. PMID: 28018444
  10. ACADS基因中的两个致病性变异导致SCAD缺乏症异常高发,以及该疾病与斯洛伐克种族结构的关系。  |  Lisyová, J., et al. 2018. BMC Med Genet. 19: 64. PMID: 29678161
  11. ACAD10和ACAD11可促进哺乳动物4-羟基酸脂质分解代谢。  |  Rashan, EH., et al. 2024. bioRxiv.. PMID: 38260250
  12. 人类短链酰基-CoA 脱氢酶基因的结构组织。  |  Corydon, MJ., et al. 1997. Mamm Genome. 8: 922-6. PMID: 9383286

订购信息

产品名称产品编号规格价格数量收藏夹

ACADS 抗体 (G-10)

sc-365953
200 µg/ml
$316.00

ACADS (G-10): m-IgGκ BP-HRP 套装

sc-522439
200 µg Ab, 40 µg BP
$354.00

ACADS (G-10): m-IgG2b BP-HRP 套装

sc-549032
200 µg Ab; 10 µg BP
$354.00

ACADS 抗体 (G-10) AC

sc-365953 AC
500 µg/ml, 25% agarose
$416.00

ACADS 抗体 (G-10) HRP

sc-365953 HRP
200 µg/ml
$316.00

ACADS 抗体 (G-10) FITC

sc-365953 FITC
200 µg/ml
$330.00

ACADS 抗体 (G-10) PE

sc-365953 PE
200 µg/ml
$343.00

ACADS 抗体 (G-10) Alexa Fluor® 488

sc-365953 AF488
200 µg/ml
$357.00

ACADS 抗体 (G-10) Alexa Fluor® 546

sc-365953 AF546
200 µg/ml
$357.00

ACADS 抗体 (G-10) Alexa Fluor® 594

sc-365953 AF594
200 µg/ml
$357.00

ACADS 抗体 (G-10) Alexa Fluor® 647

sc-365953 AF647
200 µg/ml
$357.00

ACADS 抗体 (G-10) Alexa Fluor® 680

sc-365953 AF680
200 µg/ml
$357.00

ACADS 抗体 (G-10) Alexa Fluor® 790

sc-365953 AF790
200 µg/ml
$357.00

I am using ACADS (G-10): sc-365953 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from IHCWe used it for IHC ,got strong signal of mouse kidney tissue, the staining is oberved very specificly,1:200 dilution.
Date published: 2017-08-26
Rated 2 out of 5 by from poor resultI have tested this antibody for WB of human kidney tissue extracts at a dilution of 1:200 but poor results with weak bands signal.Although tried different conditions ,but the result is no difference.
Date published: 2017-08-12
Rated 5 out of 5 by from Good resultThe specificity of ACADS antibody is very good,i have a good result by WB.
Date published: 2017-05-24
Rated 5 out of 5 by from WBright size band for acads in THP1 cells by WB.......
Date published: 2016-12-29
Rated 5 out of 5 by from WB publishable data with human Huh7WB publishable data with human Huh7.5 - SCBT Publication Review
Date published: 2015-04-02
Rated 4 out of 5 by from Strong band seen in WB using human kidneyStrong band seen in WB using human kidney tissue extract. -SCBT QC
Date published: 2015-03-29
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC
Date published: 2015-03-24
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic stainingBeautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human thyroid gland tissue. -SCBT QC
Date published: 2014-10-10
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ACADS 抗体 (G-10) is rated 4.4 out of 5 by 8.
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