Date published: 2025-9-7
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Ac-lysine Antibody (AKL5C1) - Western Blotting - Image 139610
Ac-lysine 抗体 (AKL5C1): sc-32268. 未经处理 (A) 和经 Trichostatin A (sc-3511) 处理 (B) 的 NIH/3T3 全细胞裂解液中 Ac-lysine 乙酰化的 Western 印迹分析. 注意 B 道中 Ac-lysine 乙酰化的上调.
Ac-lysine 抗体 (AKL5C1): sc-32268. 未经处理 (A) 和经 Trichostatin A (sc-3511) 处理 (B) 的 NIH/3T3 全细胞裂解液中 Ac-lysine 乙酰化的 Western 印迹分析. 注意 B 道中 Ac-lysine 乙酰化的上调.

Ac-lysine 抗体 (AKL5C1): sc-32268

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寫評論提問

说明书
  • Ac-lysine 抗体 AKL5C1 是小鼠单克隆 IgG1 κ，Ac-lysine抗体, 在67篇文献中引用，规格为200 µg/ml
  • 针对化学乙酰化的匙孔血蓝蛋白
  • Ac-lysine 抗体 (AKL5C1) 推荐用于 WB, IP, IF 和 IHC(P)，检测 proteins containing N-epsilon-acetylated lysine residues ; 不会与 N-α-acetylated lysine产生交联反应
  • Ac-lysine抗体（AKL5C1）可与琼脂糖结合用于IP
  • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Ac-lysine (AKL5C1): sc-32268的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Ac-lysine 抗体 (AKL5C1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Ac-lysine 抗体 (AKL5C1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
说明书与实验方案
研究信息

関連項目

Ac-lysine抗体（AKL5C1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测各种物种中的Ac-lysine蛋白。抗乙酰赖氨酸抗体（AKL5C1）有非结合型和多种结合型，包括琼脂糖和多种Alexa Fluor®结合物，可满足不同实验需求。赖氨酸乙酰化是Ac-lysine (AKL5C1)抗体识别的修饰，在调节基因表达和蛋白质功能方面发挥着关键作用，存在于核心组蛋白、转录因子和许多其他蛋白质中。这种可逆的修饰受乙酰转移酶和脱乙酰酶之间动态相互作用的影响，而乙酰转移酶和脱乙酰酶又对细胞信号作出反应。乙酰赖氨酸残基为含溴结构域的蛋白（如Gcn5、PCAF、TAF1和CBP）创造了特定的对接位点，这些蛋白对于招募转录共激活因子和调节染色质结构至关重要。组蛋白乙酰化，特别是组蛋白H3的N-末端，会受到Trichostatin A等抑制剂的影响，后者可提高乙酰化水平；也会受到乙醇等外部因素的影响，后者会提高特定赖氨酸残基的乙酰化水平，且剂量依赖性。Ac-lysine (AKL5C1) 单克隆抗体是研究基因调控机制和赖氨酸乙酰化在各种生物学环境中的功能意义的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ac-lysine 抗体 (AKL5C1) 参考文献:

  1. 原生复合体中 Gcn5 的赖氨酸乙酰化特异性得到扩展。  |  Grant, PA., et al. 1999. J Biol Chem. 274: 5895-900. PMID: 10026213
  2. 组蛋白 H3 中丝氨酸 10 的磷酸化在体外和体内与 Gcn5 介导的赖氨酸 14 的乙酰化有功能上的联系。  |  Lo, WS., et al. 2000. Mol Cell. 5: 917-26. PMID: 10911986
  3. 乙醇对大鼠肝细胞中组蛋白 H3 赖氨酸 9 的乙酰化作用。  |  Park, PH., et al. 2003. Biochem Biophys Res Commun. 306: 501-4. PMID: 12804592
  4. 三司他丁 A 诱导组蛋白 H3 的丝氨酸 28 磷酸化和赖氨酸 9 乙酰化。  |  Zhong, S., et al. 2003. Oncogene. 22: 5291-7. PMID: 12917630
  5. 组蛋白去乙酰化酶抑制剂通过 p53 在 320 和 373 个赖氨酸残基上的乙酰化诱导 PIG3 和 NOXA，作为细胞凋亡的一种机制。  |  Terui, T., et al. 2003. Cancer Res. 63: 8948-54. PMID: 14695212
  6. AML1 通过 p300 介导的特定赖氨酸残基乙酰化进行功能调节。  |  Yamaguchi, Y., et al. 2004. J Biol Chem. 279: 15630-8. PMID: 14752096
  7. 赖氨酸乙酰化和溴化链：信号传递的新伙伴关系。  |  Yang, XJ. 2004. Bioessays. 26: 1076-87. PMID: 15382140
  8. 通过单个赖氨酸残基的可逆乙酰化调节 Stat3 的二聚化。  |  Yuan, ZL., et al. 2005. Science. 307: 269-73. PMID: 15653507
  9. 溴结构域的结构和乙酰基赖氨酸识别。  |  Mujtaba, S., et al. 2007. Oncogene. 26: 5521-7. PMID: 17694091
  10. 心脏乙酰赖氨酸蛋白质组  |  Foster, DB., et al. 2013. PLoS One. 8: e67513. PMID: 23844019
  11. MYC 乙酰化赖氨酸残基驱动致癌细胞转化体，并调节细胞粘附无关生长和存活的选择基因程序。  |  Hurd, M., et al. 2023. Genes Dev. 37: 865-882. PMID: 37852796
  12. 在嗜热四膜虫体内，α-微管蛋白中赖氨酸 40 的乙酰化并不重要。  |  Gaertig, J., et al. 1995. J Cell Biol. 129: 1301-10. PMID: 7775576

订购信息

产品名称产品编号规格价格数量收藏夹

Ac-lysine 抗体 (AKL5C1)

sc-32268
200 µg/ml
$316.00

Ac-lysine (AKL5C1): m-IgG Fc BP-HRP 套装

sc-528450
200 µg Ab; 10 µg BP
$354.00

Ac-lysine (AKL5C1): m-IgGκ BP-HRP 套装

sc-520830
200 µg Ab, 40 µg BP
$354.00

Ac-lysine (AKL5C1): m-IgG1 BP-HRP 套装

sc-542937
200 µg Ab; 20 µg BP
$354.00

Ac-lysine 抗体 (AKL5C1) AC

sc-32268 AC
500 µg/ml, 25% agarose
$416.00

Ac-lysine 抗体 (AKL5C1) Alexa Fluor® 546

sc-32268 AF546
200 µg/ml
$357.00

Ac-lysine 抗体 (AKL5C1) Alexa Fluor® 594

sc-32268 AF594
200 µg/ml
$357.00

Ac-lysine 抗体 (AKL5C1) Alexa Fluor® 680

sc-32268 AF680
200 µg/ml
$357.00

Ac-lysine 抗体 (AKL5C1) Alexa Fluor® 790

sc-32268 AF790
200 µg/ml
$357.00

你好，请问贵公司一抗支持申请试用装吗？

Asked by: 中国唐国浩
感谢您的提问。您可以随一抗正装的订单，申请不同货号的一抗小样。 Thank you for your question. Primary antibody samples can be requested with a different primary antibody order.
Answered by: Technical Service SH
Date published: 2024-08-23

Ac-lysine 抗体 (AKL5C1): sc-32268这个抗体如果不冻起来的话，稀释一抗是要用专门的一抗稀释液吗，我可以用牛奶稀释然后加叠氮化钠吗？

Asked by: 认真做科研
感谢您的提问，这支抗体的保存条件是4℃，不可以冰冻。稀释可以用通用稀释液、稀释缓冲液或封闭试剂。建议您现配现用，不推荐稀释后长期保存。谢谢。 Thank you for your question. The storage condition of this antibody is 4℃ and cannot be frozen. Dilution can be done with a universal diluent, dilution buffer, or blocking buffer. It is recommended that dilute as you use. It is not recommended to keep it after dilution for a long time. thank you
Answered by: Jiajun Z
Date published: 2022-12-14

Is it possible to get a sample aliquot to determine if this product can detect some of my peptides before I commit to buying. Or a sample that can be less than 70$

Asked by: Ouma
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

What is the species reactivity of this antibody? Can it be used with human samples?

Asked by: AbPolly
Thank you for your inquiry. This antibody is not species specific but can be used detection of acetylated lysine residues in any sample, including human.
Answered by: Technical Support 8
Date published: 2017-01-04
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Rated 4 out of 5 by from Segnale buonoHo utilizzato quest'antiporto per WB su linee cellulari HCT15, HCT116 e LS513 ad una diluizione di 1:500, ottenendo un segnale buono con background pulito
Date published: 2025-01-08
Rated 5 out of 5 by from Nice WBWorked well when using for WB in intestinal crypt cells.
Date published: 2019-04-01
Rated 5 out of 5 by from Great in WBWhen used in whole lysate in non-milk block at 1:1,000 produces expected ladder of proteins with the most prominent bands corresponding to acetylated tubular and acetylated histones.
Date published: 2018-04-13
Rated 5 out of 5 by from Ac-lysine抗体使用情况我并没有做western,只是做IP,很遗憾并没有做出来，试了几次，我确定我要IP的蛋白是有赖氨酸残基的，但是做不出来我就只能怀疑是抗体的问题了还是？有待考究，仅供你们参考！
Date published: 2017-12-07
Rated 5 out of 5 by from Good AntibodyAcetylated lysine is very good antibody. We got good Western blot and immunoprecipitation results.
Date published: 2017-10-20
Rated 4 out of 5 by from Good antibodyWorked well with western blotting at 1:500 dilution.
Date published: 2017-09-27
Rated 4 out of 5 by from works great on western blotWe have used this antibody on human skin tissue. It works great on western blot.
Date published: 2017-09-20
Rated 4 out of 5 by from IT is very goodit is good for ip, but it will be two results. so you should know your protein ,where its ac-lysine is.
Date published: 2017-09-12
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Ac-lysine 抗体 (AKL5C1) is rated 4.5 out of 5 by 20.
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