Date published: 2025-9-6
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ABCA4 Antibody (3F4) - Immunohistochemistry - Image 378055
ABCA4 抗体 (3F4): sc-65672. 福尔马林固定, 石蜡包埋的人胎儿眼组织的免疫过氧化物酶染色显示视网膜细胞膜和细胞质染色. 请注意视杆细胞外节层的强烈染色.
ABCA4 Antibody (3F4) - Immunohistochemistry - Image 378055
ABCA4 抗体 (3F4): sc-65672. 福尔马林固定, 石蜡包埋的人胎儿眼组织的免疫过氧化物酶染色显示视网膜细胞膜和细胞质染色. 请注意视杆细胞外节层的强烈染色.
ABCA4 Antibody (3F4) - Western Blotting - Image 390784
ABCA4 抗体 (3F4) Alexa Fluor® 546: sc-65672 AF546. 直接荧光西部印迹分析小鼠眼组织提取物中 ABCA4 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
ABCA4 Antibody (3F4) - Western Blotting - Image 15736
ABCA4 抗体 (3F4): sc-65672. 小鼠眼组织提取物中 ABCA4 表达的 Western 印迹分析.
ABCA4 抗体 (3F4): sc-65672. 福尔马林固定, 石蜡包埋的人胎儿眼组织的免疫过氧化物酶染色显示视网膜细胞膜和细胞质染色. 请注意视杆细胞外节层的强烈染色.

ABCA4 抗体 (3F4): sc-65672

3.6(5)
寫評論提問

说明书
  • ABCA4抗体(3F4)是小鼠单克隆IgG1 κ, 在3篇文献中引用，规格为200 µg/ml
  • 针对bovine来源的220 kDa ABCA4蛋白
  • 抗-ABCA4 抗体 (3F4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 ABCA4; 也和以下物种反应，包括： bovine
  • ABCA4抗体（3F4）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取ABCA4 (3F4): sc-65672的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 ABCA4 抗体 (3F4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与ABCA4 抗体 (3F4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 ABC 抗体包括 ABCF1, ABCG2, ABCF2, ABCA6, ABCB9, ABCC13, ABCC11, ABCD3 和 ABCB5

ABCA4抗体（3F4）是一种IgG1 κ小鼠单克隆ABCA4抗体，可通过蛋白质印迹法、免疫沉淀法、免疫荧光法和免疫组织化学法（IHC（P））检测小鼠、大鼠和人类来源的ABCA4。ABCA4抗体（3F4）既有偶联型也有非偶联型抗ABCA4抗体形式。ATP结合盒（ABC）超家族由参与各种底物能量依赖性跨膜转运的跨膜蛋白组成。ABCA4（也称为ABCR、光感受器RIM或RMP）是一种光感受器特异性的ATP结合盒（ABC）转运蛋白。ABCA4仅在光感受器细胞中表达，表明ABCA4介导必需分子进入或离开光感受器细胞的转运。编码ABCA4的基因突变是导致常染色体隐性遗传性Stargardt病（STGD，一种早期发病的黄斑变性）以及一些形式的常染色体隐性遗传性视锥-视杆细胞营养不良和常染色体隐性遗传性视网膜色素变性的原因。此外，ABCA4突变的杂合性可能是年龄相关性黄斑变性的一个危险因素。ABCA4与小鼠和人类的ABC1和ABC2关系最为密切，定位于人类1p22.1染色体上。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ABCA4 抗体 (3F4) 参考文献:

  1. 与人类视网膜病变相关的 ABCR 蛋白变体的生化缺陷。  |  Sun, H., et al. 2000. Nat Genet. 26: 242-6. PMID: 11017087
  2. 晚发型斯塔加特病与映射到 ABCR（ABCA4）已知功能区之外的错义突变缔合作用有关。  |  Yatsenko, AN., et al. 2001. Hum Genet. 108: 346-55. PMID: 11379881
  3. 西班牙常染色体隐性黄斑营养不良症患者的 ABCA4 (ABCR) 基因突变谱。  |  Paloma, E., et al. 2001. Hum Mutat. 17: 504-10. PMID: 11385708
  4. 与囊性纤维化, 多药耐药性和细菌转运有关的 ATP 结合蛋白的结构模型。  |  Hyde, SC., et al. 1990. Nature. 346: 362-5. PMID: 1973824
  5. 德国多中心队列中 335 名斯塔加特病患者 ABCA4 基因的突变谱--精选深部内隐性变异和常见 SNPs 的影响  |  Schulz, HL., et al. 2017. Invest Ophthalmol Vis Sci. 58: 394-403. PMID: 28118664
  6. 由 ABCA4 基因突变引起的视网膜疾病的临床范围、遗传复杂性和治疗方法。  |  Cremers, FPM., et al. 2020. Prog Retin Eye Res. 79: 100861. PMID: 32278709
  7. 通过整合基因组学和转录组学，为 1054 名斯达加特病疑似患者解开 ABCA4 的谜团。  |  Khan, M., et al. 2020. Genet Med. 22: 1235-1246. PMID: 32307445
  8. 对中国大样本人群中 ABCA4 基因相关视网膜营养不良症的临床和遗传分析  |  Sun, Z., et al. 2021. Exp Eye Res. 202: 108389. PMID: 33301772
  9. ABCA4 的结构和功能及其在视觉周期和斯塔加特黄斑变性中的作用。  |  Molday, RS., et al. 2022. Prog Retin Eye Res. 89: 101036. PMID: 34954332
  10. ATP 结合盒转运基因的进化。  |  Dean, M. and Allikmets, R. 1995. Curr Opin Genet Dev. 5: 779-85. PMID: 8745077
  11. 老年性黄斑变性中的斯塔加特病基因（ABCR）突变。  |  Allikmets, R., et al. 1997. Science. 277: 1805-7. PMID: 9295268
  12. ABC 转运体促进 ATP 传导运输。  |  Schwiebert, EM. 1999. Am J Physiol. 276: C1-8. PMID: 9886914

订购信息

产品名称产品编号规格价格数量收藏夹

ABCA4 抗体 (3F4)

sc-65672
200 µg/ml
$316.00

ABCA4 (3F4): m-IgG Fc BP-HRP 套装

sc-526960
200 µg Ab; 10 µg BP
$354.00

ABCA4 (3F4): m-IgG1 BP-HRP 套装

sc-532333
200 µg Ab; 20 µg BP
$354.00

ABCA4 抗体 (3F4) AC

sc-65672 AC
500 µg/ml, 25% agarose
$416.00

ABCA4 抗体 (3F4) FITC

sc-65672 FITC
200 µg/ml
$330.00

ABCA4 抗体 (3F4) PE

sc-65672 PE
200 µg/ml
$343.00

ABCA4 抗体 (3F4) Alexa Fluor® 488

sc-65672 AF488
200 µg/ml
$357.00

ABCA4 抗体 (3F4) Alexa Fluor® 546

sc-65672 AF546
200 µg/ml
$357.00

ABCA4 抗体 (3F4) Alexa Fluor® 594

sc-65672 AF594
200 µg/ml
$357.00

ABCA4 抗体 (3F4) Alexa Fluor® 647

sc-65672 AF647
200 µg/ml
$357.00

ABCA4 抗体 (3F4) Alexa Fluor® 680

sc-65672 AF680
200 µg/ml
$357.00

ABCA4 抗体 (3F4) Alexa Fluor® 790

sc-65672 AF790
200 µg/ml
$357.00

What is the recognised epitope by this antibody?

Asked by: Laura
Thank you for your question. ABCA4 Antibody (3F4): sc-65672 was raised against 220-kDa ABCA4 protein of bovine origin. No epitope mapping has been performed for this particular antibody as of yet, so its exact binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2025-07-04

Will this anti-ABCA4 (SC-65672) bind ABCA4 ECD1 or ECD2? Need for Flow cytometry... Thx

Asked by: Anonymous
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We do not know whether or not the antibody binds to ECD1 or ECD2.
Answered by: Tech Service 1
Date published: 2020-12-10

I purchased your ABCA4 (3F4) sc-65672 antibody last year and tried it on fibroblast cells following one of the published paper but I didn't get the expected band. Can you help me with that? Thanks.

Asked by: Di Huang
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-09-11

Hello I wish to know if it is possible to recieve the 3F4 ABCA4 antibody conjugated to agarose or sepharose. And if so how the 3F4-immunoaffinity column should be prepared.

Asked by: Tess555
Thank you for your question. We provide sc-65672 conjugated to agarose for IP experiments. If you require more information, please contact our Technical Service https://www.scbt.com/scbt/resources/technical-service
Answered by: SCBT Heidelberg Support
Date published: 2018-12-22

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with ABCA4 Antibody (3F4): sc-65672?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2025-03-16
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Rated 3 out of 5 by from IF is OK, but WB no binding~~~~~~~~~IF is OK, but WB no binding in mouse retina~~~~~~~~~
Date published: 2023-03-21
Rated 3 out of 5 by from Not so good as other ABC family antibodyThe band is not clear enough, there are many inspecialized bands on the film.
Date published: 2018-01-30
Rated 4 out of 5 by from Nice westernVery clean western blot data; would like to see staining
Date published: 2017-01-10
Rated 5 out of 5 by from Reliable and Versatile AntibodyStrong, clear signal in WB with little to no background. Very Reliable. Works well in a variety of species including mouse, rat, bovine, and human samples,
Date published: 2016-12-22
Rated 3 out of 5 by from Light but positive band in WB in mouse eyeLight but positive band in WB in mouse eye tissue extract. -SCBT QC
Date published: 2014-01-09
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ABCA4 抗体 (3F4) is rated 3.6 out of 5 by 5.
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