2X Laemmli Sample Buffer For SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer.

2X Laemmli Sample Buffer

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应用; For SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer
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* 参考分析证明 大量特定数据 (包括水 含量).
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Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.


参考文献

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606

规划 :
2X
物理状态 :
Liquid
溶解度 :
Soluble in water.
pH :
~6.8
保存 :
Store at -20° C
仅供科研使用。不可用于诊断或治疗。
德国水公害等级 :
3
运输 :
UN 3082, Class 9, Packing group III

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