Date published: 2025-9-9
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20S Proteasome α3 Antibody (A-9) - Immunohistochemistry - Image 377172
20S 蛋白酶体 α3 抗体 (A-9): sc-166205. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆和膜染色.
20S 蛋白酶体 α3 抗体 (A-9): sc-166205. 福尔马林固定, 石蜡包埋的人胆囊组织的免疫过氧化物酶染色显示腺细胞的胞浆和膜染色.

20S Proteasome α3 抗体 (A-9): sc-166205

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寫評論提問

说明书
  • 20S Proteasome α3抗体(A-9)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为200 µg/ml
  • 针对氨基酸131-255的抗原，在20S Proteasome α3的C-terminus处映射，human来源
  • 抗-20S Proteasome alpha 3 抗体 (A-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 20S Proteasome α3
  • 抗20S Proteasome alpha 3抗体（A-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取20S Proteasome α3 (A-9): sc-166205的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 20S Proteasome α3 抗体 (A-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与20S Proteasome α3 抗体 (A-9) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加20S Proteasome抗体，包括20S Proteasome α和20S Proteasome β

20S蛋白酶体α3抗体（A-9）是一种IgG1 κ小鼠单克隆20S蛋白酶体α3抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的20S蛋白酶体α3。20S蛋白酶体α3抗体（A-9）既有非偶联抗20S蛋白酶体α3抗体形式，也有多种偶联形式的抗20S蛋白酶体α3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。蛋白酶体是一个大型蛋白质复合物，存在于所有真核生物、古生菌以及部分细菌中。蛋白酶体的主要功能是通过蛋白质水解降解不必要的或受损的蛋白质。蛋白酶体最常见的形式是26S蛋白酶体，它包含一个20S蛋白酶体核心颗粒结构和两个19S调节帽。20S蛋白酶体核心是中空的，形成一个封闭的腔室，蛋白质在其中降解，同时两端有开口允许目标蛋白质进入。20S蛋白酶体核心颗粒含有许多亚基，具体取决于生物体。所有这些亚基都属于两种类型之一：α亚基，它是结构性的，作为调节颗粒的对接域和阻止不受调节地进入内部腔室的外部门；或β亚基，它主要是催化性的。蛋白酶体中的外两个环各由七个α亚基组成，而内两个环各由七个β亚基组成。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

20S Proteasome α3 抗体 (A-9) 参考文献:

  1. 水稻（Oryza sativa）20S 蛋白酶体亚基的主要结构特征。  |  Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
  2. 抗蛋白酶体抗体的诊断意义。  |  Feist, E., et al. 2000. Int Arch Allergy Immunol. 123: 92-7. PMID: 11014975
  3. 20S蛋白酶体α亚基C8（α7）的磷酸化稳定了26S蛋白酶体，并在γ干扰素调节蛋白酶体复合物方面发挥作用。  |  Bose, S., et al. 2004. Biochem J. 378: 177-84. PMID: 14583091
  4. 4-hydroxynonenal 对 20S 蛋白酶体催化位点的特异性抑制。  |  Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
  5. 利用乙烯基砜和 2-酮基-1,3,4-恶二唑优化与人类 20S 蛋白酶体 beta5 亚基的亚位结合：强效选择性蛋白酶体抑制剂的合成与细胞特性。  |  Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
  6. 基于凝胶的化学交联分析乳腺癌中 20S 蛋白酶体亚基与亚基之间的相互作用。  |  Song, H., et al. 2016. J Huazhong Univ Sci Technolog Med Sci. 36: 564-570. PMID: 27465334
  7. 牛 20S 蛋白酶体结晶的有序结构。  |  Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
  8. 20S 蛋白酶体降解蛋白质过程中的构象限制。  |  Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
  9. 20S 蛋白酶体的结构和结构形成。  |  Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
  10. 通过部分测序从二维凝胶中鉴定的人类蛋白酶体亚基。  |  Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
  11. 对 20S 蛋白酶体复合物的全面质谱分析。  |  Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
  12. 超嗜热古生物暴怒嗜热菌中的 20S 蛋白酶体 beta1 亚基在其功能和稳定性中的作用。  |  Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253

订购信息

产品名称产品编号规格价格数量收藏夹

20S Proteasome α3 抗体 (A-9)

sc-166205
200 µg/ml
$316.00

20S Proteasome α3 (A-9): m-IgG Fc BP-HRP 套装

sc-528920
200 µg Ab; 10 µg BP
$354.00

20S Proteasome α3 (A-9): m-IgGκ BP-HRP 套装

sc-521532
200 µg Ab, 40 µg BP
$354.00

20S Proteasome α3 (A-9): m-IgG1 BP-HRP 套装

sc-543211
200 µg Ab; 20 µg BP
$354.00

20S Proteasome α3 抗体 (A-9) AC

sc-166205 AC
500 µg/ml, 25% agarose
$416.00

20S Proteasome α3 抗体 (A-9) HRP

sc-166205 HRP
200 µg/ml
$316.00

20S Proteasome α3 抗体 (A-9) FITC

sc-166205 FITC
200 µg/ml
$330.00

20S Proteasome α3 抗体 (A-9) PE

sc-166205 PE
200 µg/ml
$343.00

20S Proteasome α3 抗体 (A-9) Alexa Fluor® 488

sc-166205 AF488
200 µg/ml
$357.00

20S Proteasome α3 抗体 (A-9) Alexa Fluor® 546

sc-166205 AF546
200 µg/ml
$357.00

20S Proteasome α3 抗体 (A-9) Alexa Fluor® 594

sc-166205 AF594
200 µg/ml
$357.00

20S Proteasome α3 抗体 (A-9) Alexa Fluor® 647

sc-166205 AF647
200 µg/ml
$357.00

20S Proteasome α3 抗体 (A-9) Alexa Fluor® 680

sc-166205 AF680
200 µg/ml
$357.00

20S Proteasome α3 抗体 (A-9) Alexa Fluor® 790

sc-166205 AF790
200 µg/ml
$357.00

I want to order an antibody against the 20S proteasome for immunofluorescence conjugated to Alexafluor 647. How do I specify that I want it to be conjugated in the order?

Asked by: Jdub
Thank you for your question. The conjugated antibodies contain a suffix (e.g., AF###) after the catalog number (sc-#) of the parent (unconjugated) antibody product. The catalog number of the product you want to order is: sc-166205 AF647. Product description is 20S Proteasome α3 Antibody (A-9) Alexa Fluor® 647. If you are placing your order on our website, scroll down to the "Ordering Information" and all of the different conjugated versions of the antibody are listed in a table. You can add "sc-166205 AF647" to your cart.
Answered by: Technical Support
Date published: 2019-01-05

Can 20S Proteasome α3 (A-9): sc-166205 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes, 20S Proteasome α3 (A-9): sc-166205 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Great features.We used this antibody in rat brain hippocampal cytosolic fraction (1:500), and we got clear bands.
Date published: 2020-12-29
Rated 4 out of 5 by from 抗体试用反馈申请psma3单克隆抗体小样。western-blot结果显示很满意。图示条带即为psma3。无杂带
Date published: 2018-05-04
Rated 5 out of 5 by from Good Western Blot data of 20S ProteasomeGood Western Blot data of 20S Proteasome 3 expression in A549 and JAR whole cell lysates and mouse heart and rat placenta tissue extracts. -SCBT QC
Date published: 2013-06-13
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20S Proteasome α3 抗体 (A-9) is rated 4.7 out of 5 by 3.
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