Date published: 2025-9-6

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15-LO2 抗体 (D-9): sc-271290

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说明书
  • 15-LO2 抗体 D-9 是小鼠单克隆 IgG1 κ,15-LO2抗体, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的15-LO2的内部区域内的氨基酸423-452之间
  • 15-LO2 抗体 (D-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 15-LO2 splice variants a, b and c
  • 抗15-LO2抗体(D-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取15-LO2 (D-9): sc-271290免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 15-LO2 抗体 (D-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与15-LO2 抗体 (D-9) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

15-LO2抗体(D-9)是一种IgG1 κ小鼠单克隆15-LO2抗体(也称为15-LO2抗体),可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学(石蜡)和酶联免疫吸附试验检测小鼠、大鼠和人类来源的15-LO2剪接变异体a、b和c蛋白蛋白。15-LO2抗体(D-9)既有非偶联抗15-LO2抗体形式,也有多种偶联形式的抗15-LO2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。脂氧合酶是一类对不饱和脂肪酸加双氧的酶,从而引发膜脂质过氧化、合成信号分子以及诱导细胞结构和代谢变化。哺乳动物的Lox酶,12-LO和15-LO,是按照其最常见底物花生四烯酸加双氧的位置特异性来进行分类的。花生四烯酸的代谢会产生具有生物活性的代谢产物,这些代谢产物与细胞生长和增殖以及存活和凋亡有关。15-脂氧合酶在生理学上重塑膜以及在动脉粥样硬化、炎症和癌变的发病机理中起作用。它受到高度调控,它能组织和细胞类型特异性表达。IL-4和IL-13在反式激活15-LO基因中起重要作用。15-脂氧合酶I型在前列腺癌中会过表达,它通过调节IGF-1R的表达和激活作用促进癌症进展。15-脂氧合酶II型(15-LO2)对于将花生四烯酸转化为15S-羟过氧化二十碳四烯酸很重要。它是一种细胞质蛋白,主要在角膜、肺、毛发和前列腺中表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

15-LO2 抗体 (D-9)

sc-271290
200 µg/ml
$316.00

15-LO2 (D-9): m-IgG Fc BP-HRP 套装

sc-529157
200 µg Ab; 10 µg BP
$354.00

15-LO2 (D-9): m-IgGκ BP-HRP 套装

sc-521903
200 µg Ab, 40 µg BP
$354.00

15-LO2 (D-9): m-IgG1 BP-HRP 套装

sc-543359
200 µg Ab; 20 µg BP
$354.00

15-LO2 抗体 (D-9) AC

sc-271290 AC
500 µg/ml, 25% agarose
$416.00

15-LO2 抗体 (D-9) HRP

sc-271290 HRP
200 µg/ml
$316.00

15-LO2 抗体 (D-9) FITC

sc-271290 FITC
200 µg/ml
$330.00

15-LO2 抗体 (D-9) PE

sc-271290 PE
200 µg/ml
$343.00

15-LO2 抗体 (D-9) Alexa Fluor® 488

sc-271290 AF488
200 µg/ml
$357.00

15-LO2 抗体 (D-9) Alexa Fluor® 546

sc-271290 AF546
200 µg/ml
$357.00

15-LO2 抗体 (D-9) Alexa Fluor® 594

sc-271290 AF594
200 µg/ml
$357.00

15-LO2 抗体 (D-9) Alexa Fluor® 647

sc-271290 AF647
200 µg/ml
$357.00

15-LO2 抗体 (D-9) Alexa Fluor® 680

sc-271290 AF680
200 µg/ml
$357.00

15-LO2 抗体 (D-9) Alexa Fluor® 790

sc-271290 AF790
200 µg/ml
$357.00

15-LO2 (D-9) 中和勝肽

sc-271290 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-271290 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from it works well for WBthe expression of 15-lox-2 in Hek293 and hela cell transfected with 15-lox-2 Plasmid can be meassured by WB using 15-lo2 Ab with 1:100.
Date published: 2018-09-18
Rated 4 out of 5 by from Clean bands but two of them...I got this antibody as a free sample to detect endogenous 15-LO2 in human placenta samples. It has a clean band at the expected molecular weight of 75kda but it also has a distinct band around 55kDa, any idea of what is this second band? This second band is stronger in certain samples not all.
Date published: 2017-10-20
Rated 5 out of 5 by from amazingused with cell line and animal tissue, dilution is 1:500
Date published: 2016-11-19
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Nice Western Blot data of 15Nice Western Blot data of 15-LO2 expression in non-transfected and human 15-LO2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-01-12
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15-LO2 抗体 (D-9) is rated 4.8 out of 5 by 5.
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