Date published: 2025-9-7
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15-LO Antibody (B-7) - Western Blotting - Image 382215
15-LO 抗体 (B-7): sc-133085. 15-LO 在 NIH/3T3 (A), c4 (B), AMJ2-C8 (C), EOC 20 (D), MCF7 (E) 和 PC-12 (F) 全细胞裂解液中的表达的 Western 印迹分析.
15-LO 抗体 (B-7): sc-133085. 15-LO 在 NIH/3T3 (A), c4 (B), AMJ2-C8 (C), EOC 20 (D), MCF7 (E) 和 PC-12 (F) 全细胞裂解液中的表达的 Western 印迹分析.

15-LO 抗体 (B-7): sc-133085

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寫評論提問

说明书
  • 15-LO 抗体 B-7 是小鼠单克隆 IgG1 κ，15-LO抗体, 在23篇文献中引用，规格为200 µg/ml
  • 抗酸 428-662 定位在 C-terminus of 15-LO of human 起源
  • 15-LO 抗体 (B-7) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 15-LO and leukocyte-, platelet- and epidermal-type 12-LO
  • 抗15-LO抗体（B-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取15-LO (B-7): sc-133085的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是15-LO Antibody (B-7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与15-LO Antibody (B-7) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加LO抗体，包括12-LO, 15-LO, 15-LO2和5-LO

15-LO抗体（B-7）是一种IgG1 κ小鼠单克隆15-LO抗体（也称为LOX15抗体、花生四烯酸15-脂氧合酶抗体、15-LOX-1抗体、多不饱和脂肪酸脂氧合酶ALOX15抗体、花生四烯酸12-脂氧合酶白细胞型抗体、花生四烯酸Omega-6脂氧合酶抗体、Hepoxilin A3合成酶Alox15抗体、15-LOX抗体、LOG15抗体、15-脂氧合酶1型抗体或15-脂氧合酶-1抗体），可通过WB, IP, IF和ELISA检测小鼠、大鼠和人类来源的15-LO蛋白。15-LO抗体（B-7）既有非偶联形式的抗15-LO抗体，也有多种偶联形式的抗15-LO抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。脂氧合酶是一类能使不饱和脂肪酸双氧化的酶，从而引发膜的脂质过氧化和信号分子的合成，以及诱导细胞中的结构和代谢变化。哺乳动物中的Lox酶包括12-LO和15-LO，它们是根据其最常见底物花生四烯酸的脱氧位置特异性进行分类的。花生四烯酸的代谢导致产生具有生物活性的代谢产物，这些代谢产物已涉及细胞生长和增殖，以及生存和凋亡。15-脂氧合酶（15-LO）在生理性膜重塑以及动脉粥样硬化、炎症和癌变的发病机理中发挥作用。它以组织和细胞类型特异性的方式受到高度调控和表达。IL-4和IL-13在反式激活15-LO基因中起重要作用。15-LO 1型在前列腺癌中的过度表达通过调节IGF-1R的表达和激活来促进癌症进展。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

15-LO 抗体 (B-7) 参考文献:

  1. 血小板型 12-脂氧合酶的过表达可通过增强 alpha(v)beta(3) 和 alpha(v)beta(5) 整合素的表达来促进肿瘤细胞的存活。  |  Pidgeon, GP., et al. 2003. Cancer Res. 63: 4258-67. PMID: 12874035
  2. 15-脂氧合酶-1的表达能上调并激活前列腺癌细胞中的胰岛素样生长因子-1受体。  |  Kelavkar, UP. and Cohen, C. 2004. Neoplasia. 6: 41-52. PMID: 15068670
  3. 启动子甲基化对 15 脂氧合酶表达的转录调控。  |  Liu, C., et al. 2004. Exp Cell Res. 297: 61-7. PMID: 15194425
  4. 实验和临床条件下血小板型 12-脂氧合酶在人类黑色素瘤进展过程中的分子鉴定, 定位和功能。  |  Rásó, E., et al. 2004. Melanoma Res. 14: 245-50. PMID: 15305153
  5. 阿司匹林可增强人体血小板 12-脂氧合酶对 15-HETE 抑制作用的敏感性，15-HETE 是一种内源性调节剂。  |  Fletcher-Cieutat, M., et al. 1985. Prostaglandins Leukot Med. 18: 255-9. PMID: 3925465

订购信息

产品名称产品编号规格价格数量收藏夹

15-LO 抗体 (B-7)

sc-133085
200 µg/ml
$316.00

15-LO (B-7): m-IgG Fc BP-HRP 套装

sc-528744
200 µg Ab; 10 µg BP
$354.00

15-LO (B-7): m-IgGκ BP-HRP 套装

sc-521227
200 µg Ab, 40 µg BP
$354.00

15-LO (B-7): m-IgG1 BP-HRP 套装

sc-543108
200 µg Ab; 20 µg BP
$354.00

15-LO 抗体 (B-7) AC

sc-133085 AC
500 µg/ml, 25% agarose
$416.00

15-LO 抗体 (B-7) HRP

sc-133085 HRP
200 µg/ml
$316.00

15-LO 抗体 (B-7) FITC

sc-133085 FITC
200 µg/ml
$330.00

15-LO 抗体 (B-7) PE

sc-133085 PE
200 µg/ml
$343.00

15-LO 抗体 (B-7) Alexa Fluor® 488

sc-133085 AF488
200 µg/ml
$357.00

15-LO 抗体 (B-7) Alexa Fluor® 546

sc-133085 AF546
200 µg/ml
$357.00

15-LO 抗体 (B-7) Alexa Fluor® 594

sc-133085 AF594
200 µg/ml
$357.00

15-LO 抗体 (B-7) Alexa Fluor® 647

sc-133085 AF647
200 µg/ml
$357.00

15-LO 抗体 (B-7) Alexa Fluor® 680

sc-133085 AF680
200 µg/ml
$357.00

15-LO 抗体 (B-7) Alexa Fluor® 790

sc-133085 AF790
200 µg/ml
$357.00

稀释比例是多少

Asked by: MILK
Thank you for your question. Western Blotting (starting dilution 1:100, dilution range 1:100-1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range 1:30-1:3000).
Answered by: Technical Service SH
Date published: 2025-08-07

I would like to use this antibody to perform flow cytometry on both mouse and human samples. I can't find any references showing it's use in flow cytometry, does it work for this application?

Asked by: Snoble
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions
Answered by: Blake J
Date published: 2022-08-08

I have a question. Does this antibody react to both Alox12 (platelet type 12-LO) and Alox15 (white blood cell type 12-LO (12/15-LO)) in mice? What is the recommended dilution ratio when staining with frozen sections?

Asked by: Tachi
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Hi, It says that this antibody detect platelet and leukocyte 12 lo. Does that mean that it detect by IF ALOX 15 and ALOX12

Asked by: MiJi
Thank you for your question. Yes, this antibody can detect 15-LO and leukocyte-, platelet- and epidermal-type 12-LO by immunofluorescence.
Answered by: Tech Service
Date published: 2019-12-04

I would like to use this antibody to perform immunoprecipitation, but couldn't find any references for that. Does it really work? Thanks!

Asked by: Alexa
This primary antibody is recommended and warrantied for immunoprecipitation (IP), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-02-10

I'd like to use this antibody to detect the protein 15-LO, in other words, 15-LOX-1. So I wonder if this antibody can also be able to detect 15-LO2 which would be bad for our specific research. Thanks!

Asked by: victor0gyz
Thank you for your question. This mouse monoclonal antibody is specific for detection of 15-LO and is not expected to cross-react with 15-LO2. We have not experimentally validated this, but based on the epitope the antibody is specific for 15-LO.
Answered by: Technical Support
Date published: 2018-11-22

I need to use an antibody to detect the protein in samples of equine origin. Do you know if sc-133085: 15-LO (B-7) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support
Date published: 2017-03-06
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Rated 1 out of 5 by from WB doesn't work for human or mouse samplesTried this antibody for both mouse and human epithelial cell lysates and had no bands. Another 15-LO from another company, I got beautiful bands. I'll have to go back to the previous company
Date published: 2018-10-11
Rated 4 out of 5 by from Works as advertisedI used a free sample aliquot and it works as expected.
Date published: 2018-01-02
Rated 2 out of 5 by from A signal of a doublet?I got this antibody as a free sample to detect endogenous 15-LO in human placenta lysates. I see a doublet above the predicted 75kDa weight and some other bands above 100kDa and above 150kDa. Not sure if the doublet bands above 75kda are the right ones.
Date published: 2017-10-20
Rated 5 out of 5 by from western blotsWe're very happy with the antibodies. Our regular application is in western blots with mouse kidney extract, We use it in dilution 1:800.
Date published: 2017-07-22
Rated 5 out of 5 by from 15-LO (B-7): sc-133085I tried this antibody one month ago.It was clear and nice in western blot.
Date published: 2017-02-08
Rated 5 out of 5 by from Works as expected in WBThis 15-LO monoclonal antibody gives satisfactory bands for WB on NIH/3T3 and KNRK whole cell lysates at a 1:200 dilutions
Date published: 2017-01-27
Rated 5 out of 5 by from Satisfactory Western Blot data of 15Satisfactory Western Blot data of 15-LO expression in HEK293 cells transfected with human 15-LO expression vector and HEK923 cells . -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Produced positive Western Blot data of 15Produced positive Western Blot data of 15-LO expression in human PBL, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2013-04-10
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15-LO 抗体 (B-7) is rated 4.0 out of 5 by 8.
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