β-defensin 131 Double Nickase Plasmid (h): sc-416882-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- β-defensin 131 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- β-defensin 131 Double Nickase Plasmid (h) and β-defensin 131 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DEFB131. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-defensin 131 Double Nickase Plasmid (h) | sc-416882-NIC | 20 µg | $410.00 |
DEFB131 encodes human β-defensin 131, a small cationic antimicrobial peptide that contributes to innate mucosal immunity by directly disrupting microbial membranes and modulating host inflammatory signaling. As a defensin family member, β-defensin 131 is linked to epithelial barrier function and chemotactic cues that influence recruitment and activation of immune cells, intersecting with pathways governing host–microbe interactions and local cytokine responses. Altered defensin expression patterns have been studied in the context of susceptibility to infection and dysregulated inflammation at barrier tissues, making DEFB131 relevant for investigations of mucosal homeostasis and immune regulation.
β-defensin 131 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DEFB131 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DEFB131. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DEFB131 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DEFB131-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.