Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
α3C Tubulin CRISPR Activation Plasmid (h): sc-400020-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- α3C Tubulin CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- α3C Tubulin CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by α3C Tubulin CRISPR Activation Plasmid (h) and α3C Tubulin CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the TUBA3C transcriptional start site. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
α3C Tubulin CRISPR Activation Plasmid (h) | sc-400020-ACT | 20 µg | $397.00 | |||
α3C Tubulin CRISPR Activation Plasmid (h2) | sc-400020-ACT-2 | 20 µg | $397.00 |
TUBA3C encodes human α3C tubulin, a core α-tubulin isotype that polymerizes with β-tubulin to form microtubules essential for cytoskeletal organization, mitotic spindle assembly, and intracellular transport. Microtubule dynamics coordinate cell cycle progression, centrosome function, and polarized trafficking, influencing processes such as neurite outgrowth and cell migration. Perturbations in tubulin expression or microtubule homeostasis are linked to chromosomal instability and altered sensitivity to microtubule-targeting agents, making tubulin isotypes relevant in studies of proliferative and neurobiological phenotypes. As a structural component of the tubulin pool, α3C tubulin provides a tractable node for dissecting cytoskeleton-dependent signaling and stress responses.
α3C Tubulin CRISPR Activation Plasmid (h) provides a targeted, non-destructive approach to upregulating endogenous TUBA3C expression without altering the underlying DNA sequence.
α3C Tubulin CRISPR Activation Plasmid (h) is a three-plasmid synergistic activation mediator (SAM) system engineered for highly efficient, site-specific transcriptional upregulation of the TUBA3C locus in human cell lines. The system is built around a catalytically inactive Cas9 (dCas9) carrying two inactivating mutations (D10A and N863A) that eliminate nuclease activity while preserving DNA binding. This dCas9 is fused to VP64, a potent transcriptional activator, and is co-expressed with a blasticidin resistance gene for selection. The second plasmid encodes the MS2-p65-HSF1 fusion protein, a secondary activator complex that works in concert with dCas9-VP64, alongside a hygromycin resistance gene. The third plasmid encodes a target-specific 20 nt sgRNA fused to two MS2 RNA aptamers that recruit the MS2-p65-HSF1 complex to the activation site, accompanied by a puromycin resistance gene. The three plasmids are delivered at a 1:1:1 mass ratio for balanced expression of all system components.
Once assembled at the target locus, the SAM complex binds within approximately 200 bp upstream of the TUBA3C transcriptional start site, where VP64, p65, and HSF1 act in concert to recruit transcriptional machinery and drive upregulation of endogenous α3C Tubulin expression. Unlike nuclease-active Cas9, dCas9 does not introduce double-strand breaks or modify the genomic sequence, preserving the native TUBA3C locus and enabling the study of α3C Tubulin-dependent transcriptional responses at the endogenous locus, making it a valuable tool for functional studies, target gene identification, and the modeling of α3C Tubulin pathway restoration in tumor cells with silenced or reduced TUBA3C expression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.