Sulpho NHS biotin (CAS 119616-38-5)
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Sulpho NHS biotin (Sulfosuccinimidyl biotin) is a water soluble biotinylation reagent used for simple and efficient labeling of antibodies, proteins and primary amine-containing molecules. Biotin is a vitamin which binds with high affinity to avidin and streptavidin proteins. Biotin is small (244 Da), and may be conjugated to proteins without altering their biological activities. NHS-activated biotins couple to primary amino groups (-NH2) in pH 7-9 buffers to form stable amide bonds. Sulpho NHS biotin has also been used for labeling of membrane proteins due to the charge of the sodium sulfoxide group on the succinimidyl ring, it cannot permeate the cell membrane. If the cell remains intact, only primary amines exposed on the surface will be biotinylated. Recommended for use with avidin-conjugated fluorescent molecules: avidin-FITC, sc-2865 avidin-PE, sc-2876 avidin-APC, sc-2898 avidin-PE-Cy7, sc-3769 avidin-APC-Cy7, sc-3780 DY-550-avidin, sc-221567 DY-630-avidin, sc-221572.
Sulpho NHS biotin (CAS 119616-38-5) References
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Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Sulpho NHS biotin, 100 mg | sc-258187 | 100 mg | $262.00 |