Bromophenol Blue An electrophoresis tracking dye also used as an acid-base indicator

Bromophenol Blue (CAS 115-39-9)

Bromophenol Blue | CAS 115-39-9 is rated 5.0 out of 5 by 1.
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Synonym: 3',3'',5',5''-Tetrabromophenolsulfonephthalein
Application: An electrophoresis tracking dye also used as an acid-base indicator
CAS Number: 115-39-9
Molecular Weight: 669.96
Molecular Formula: C19H10Br4O5S
* Refer to Certificate of Analysis for lot specific data (including water content).
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Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis it can be used as a color marker and as as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.


References

1. Menzies, D.W. 1961. Stain Technol. 36: 285-287. PMID: 14472719
2. Flores, R., et al. 1978. Anal. Biochem. 88: 605-611. PMID: 697029

Physical State :
Solid
Solubility :
Freely soluble in NaOH. More soluble in methy and ethyl alcohols ,benzene, and in acetic acid.Slightly soluble in water (0.4g/100g) at 20° C.
Storage :
Store at room temperature
Melting Point :
270° C (dec.)
Boiling Point :
~605.6° C at 760 mmHg (Predicted)
Density :
~2.2 g/cm3 (Predicted)
Refractive Index :
n20D 1.74
IC50 :
HIV-1(RF): EC5050 = >50 µM (CEM-SS); HIV-1(RF): IC50 = >50 µM (CEM-SS)
pK Values :
pKa: 4.0
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SJ7453000
PubChem CID :
8272
Merck Index :
14: 1444
MDL Number :
MFCD00005875
EC Number :
204-086-2
Beilstein Registry :
61698
SMILES :
C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br

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Certificate of Analysis

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Bromophenol Blue  Product Citations

See how others have used Bromophenol Blue. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 17 total

PMID: # 26481421  Gaddes, ER. et al. 2015. Experimental cell research. 339: 417-26.

PMID: # 25114379  Tancharoen, S. et al. 2014. Mediators of inflammation. 2014: 754069.

PMID: # 24962405  Ahn, JH. et al. 2014. J. Vet. Sci. 15: 335-42.

PMID: # 22449796  Tamagawa, Y. et al. 2012. Laboratory investigation; a journal of technical methods and pathology. 92: 896-909.

PMID: # 21689752  Souvenir, R. et al. 2011. Neurobiol. Dis. 44: 28-37.

PMID: # 20010935  Campenot, RB. et al. 2009. Nature protocols. 4: 1869-87.

PMID: # 15115814  Hossain, MA. et al. 2004. J Neurosci. 24(17): 4187-4196.

PMID: # 12566111  Xu, Y. et al. 2003. Cardiovasc. Res. 57: 388-394.

PMID: # 12181130  Shimizu, K. et al. 2002. Am. J. Physiol. Heart Circ. Physiol. 283: H1005-H1011.

PMID: # 12235117  Maedler, K. et al. 2002. J. Clin. Invest. 110: 851-860.

Citations 1 to 10 of 17 total
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Rated 5 out of 5 by from Various publications cite the use of bromophenol Various publications cite the use of bromophenol blue in Zymography and Western Blot assays. -SCBT Publication Review
Date published: 2015-03-08
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