Bromophenol Blue Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain.

Bromophenol Blue  (CAS 115-39-9)

Bromophenol Blue is rated 5.0 out of 5 by 1.
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| See 17 Citations
Synonym: 3',3'',5',5''-Tetrabromophenolsulfonephthalein
Application: An electrophoresis tracking dye also used as an acid-base indicator
CAS Number: 115-39-9
Molecular Weight: 669.96
Molecular Formula: C19H10Br4O5S
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
Bromophenol Blue sc-24971 25 g $68.00
Bromophenol Blue sc-24971A 100 g $136.00

Bromophenol blue is useful as a tracking dye in electrophoresis, an industrial dye, a laboratory acid-base indicator and a biological stain. In gel electrophoresis it can be used as a color marker and as as a biological stain it can be used to stain proteins and nucleic acids. Bromophenol blue is an intermediate and is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue.


References

1. Menzies, D.W. 1961. Stain Technol. 36: 285-287. PMID: 14472719
2. Flores, R., et al. 1978. Anal. Biochem. 88: 605-611. PMID: 697029

Physical State :
Solid
Solubility :
Freely soluble in NaOH. More soluble in methy and ethyl alcohols ,benzene, and in acetic acid.Slightly soluble in water (0.4g/100g) at 20 °C.
Storage :
Store at room temperature
Melting Point :
273 °C
Boiling Point :
605.61 °C at 760 mmHg
Density :
2.2 g/cm3
Refractive Index :
n20D 1.74
IC50 :
HIV-1(RF): EC5050 = >50 µM (CEM-SS ); HIV-1(RF): IC50 = >50 µM (CEM-SS )
pK Values :
pKa: 4.0
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
SJ7453000
PubChem CID :
8272
Merck Index :
14: 1444
MDL Number :
MFCD00005875
EC Number :
204-086-2
Beilstein Registry :
61698
SMILES :
C1=CC=C2C(=C1)C(OS2(=O)=O)(C3=CC(=C(C(=C3)Br)O)Br)C4=CC(=C(C(=C4)Br)O)Br

Download SDS (MSDS)

Certificate of Analysis

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Bromophenol Blue Product Citations

See how others have used Bromophenol Blue. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 17 total

PMID: # 26481421
Gaddes, ER. et al. 2015. Regulation of fibrin-mediated tumor cell adhesion to the endothelium using anti-thrombin aptamer. Experimental cell research. 339: 417-26.

PMID: # 25114379
Tancharoen, S. et al. 2014. Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation. Mediators of inflammation. 2014: 754069.

PMID: # 24962405
Ahn, JH. et al. 2014. Comparison of arylalkylamine N-acetyltransferase and melatonin receptor type 1B immunoreactivity between young adult and aged canine spinal cord. Journal of veterinary science. 15: 335-42.

PMID: # 22449796
Tamagawa, Y. et al. 2012. Notch signaling pathway and Cdx2 expression in the development of Barrett's esophagus. Laboratory investigation; a journal of technical methods and pathology. 92: 896-909.

PMID: # 20010935
Campenot, RB. et al. 2009. Production of compartmented cultures of rat sympathetic neurons. Nature protocols. 4: 1869-87.

PMID: # 17980711
Doyle, AE. et al. 2008. Catechol-O-methyltransferase: effects of the val108met polymorphism on protein turnover in human cells. Biochim. Biophys. Acta. 1780: 27-33.

PMID: # 15115814
Hossain, MA. et al. 2004. Neuronal pentraxin 1: a novel mediator of hypoxic-ischemic injury in neonatal brain J Neurosci. 24(17): 4187-4196.

PMID: # 12566111
Xu, Y. et al. 2003. Estrogen modulation of left ventricular remodeling in the aged heart. Cardiovasc. Res.. 57: 388-394.

PMID: # 12181130
Shimizu, K. et al. 2002. MitoK(ATP) opener, diazoxide, reduces neuronal damage after middle cerebral artery occlusion in the rat. Am. J. Physiol. Heart Circ. Physiol.. 283: H1005-H1011.

PMID: # 12235117
Maedler, K. et al. 2002 . Glucose-induced beta cell production of IL-1beta contributes to glucotoxicity in human pancreatic islets J. Clin. Invest.. 110: 851-860.

Citations 1 to 10 of 17 total
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Rated 5 out of 5 by from Various publications cite the use of bromophenol Various publications cite the use of bromophenol blue in Zymography and Western Blot assays. -SCBT Publication Review
Date published: 2015-03-08
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