acetylated alpha Tubulin Antibody (6-11B-1) recommended for detection of acetylated α Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P)

acetylated α Tubulin Antibody (6-11B-1): sc-23950

acetylated α Tubulin Antibody (6-11B-1) is rated 4.8 out of 5 by 38.
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Datasheets
    • acetylated α Tubulin Antibody (6-11B-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against the outer arms of Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
    • recommended for detection of acetylated α Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P); non cross-reactive with non-acetylated α Tubulin or other lysine acetylation sites
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of acetylated α Tubulin (6-11B-1): sc-23950.
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We have purchased and used acetylated α Tubulin Antibody (6-11B-1): sc-23950 antibody. It is working quite well. However we need to know the which amino acid residue the antibody is reacting with? Kindly tell me particular epitope to which ab binds.

Asked by: Nitish
Thank you for your question. This mouse monoclonal antibody recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40 (Lysine 40).
Answered by: Technical Support
Date published: 2019-05-03

Hi, I would like to know which light chain is in this antibody (e.g, kappa I, II or V). I would like to undertsand if I can purify the antibody with protein L resin. I would like to find out the affinity of the antibody with protein L. Thank you!

Asked by: Foram
Thank you for your question. Since acetylated α Tubulin Antibody (6-11B-1): sc-23950 is a mouse IgG2 antibody it can be purified using Protein A or Protein A/G. For further information please contact our technical service directly.
Answered by: Technical Support Europe
Date published: 2019-02-04

Hi, I am interested in co-staining all microtubules (a-tubulin ab) and acetylated ones (acetylated a-tubulin). The issue is that both are mouse. Would a mouse secondary detect a conjugated acetylated a-tub antibody?

Asked by: JuanManuelGomez
Thank you for your question. We would not recommend co-staining with one conjugated primary antibody, and one un-conjugated primary antibody combined with an anti-mouse secondary antibody. There would still be the possibility of cross-reactivity between the anti-mouse IgG and the conjugated mouse monoclonal antibody. Instead, we would recommend co-staining with two conjugated primary antibodies, if both are mouse IgG.
Answered by: Technical Support
Date published: 2017-05-16

Dear Santa Cruz! I would like to stain the primary cilium of HK2 cells with sc-23950. I saw a cytoskeletal network on one picture and individual cilia on the other. How can I achieve the cilia staining, with no visibility of the cytoskeleton? Thank you

Asked by: mkeszthelyi
Thank you for your question. We do not have a specific protocol for using our monoclonal acetylated α Tubulin Antibody (6-11B-1): sc-23950 for detection of individual cilium exclusively. Provided below is a link to our recommended protocol for immunofluorescence and immunoperoxidase staining. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining This antibody has also been cited in the literature at least 30 times, many of which are using the antibody for IF or IHC applications. For example, PMID's: 27062996, 26079437 and 25286302. We hope that some of these publications may be useful in identifying a protocol for your research needs.
Answered by: Technical Support
Date published: 2017-03-01

What is the acetylation site that is recognized by this antibody?  Will it detect non-acetylated α Tubulin?

Asked by: Dr Ninau Qelp
acetylated α Tubulin Antibody (6-11B-1): sc-23950 recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40.
Answered by: Tech Service 2
Date published: 2016-12-15

What is the recommended antigen-retrieval for IHC?

Asked by: Dr Ninau Qelp
We recommend 10 mM sodium citrate buffer, pH 6.0 and heating for 5 minutes at 95° C.
Answered by: Tech Service 2
Date published: 2017-09-27
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Rated 5 out of 5 by from Primary cilia - Immunofluorescence The antibody works quite well and is specific to the target.
Date published: 2019-05-28
Rated 5 out of 5 by from Very good for WB and IF I got a sample for test. It has very nice signal in western Blot. I loved the immunofluorescence images. Now I bought 1ml of this antibody and it works great.
Date published: 2019-04-22
Rated 4 out of 5 by from good signal in IF experiments this antibody can give a good IF signal in some tissues.
Date published: 2019-02-05
Rated 4 out of 5 by from anti-tubulin, K40 acetylated Very good antibody for the detection of K40-acetylated alpha-tubulin by IF.
Date published: 2018-11-14
Rated 4 out of 5 by from Good antibody! I received a sampler size for this antibody and it worked really well 1:1000 in U373 GBM whole cell extracts.
Date published: 2018-11-08
Rated 5 out of 5 by from very strong immunofluprescence signal Very strong immunofluorescent signals were detected specifically in cilia when we stained mouse lung tissue slides mounted in OCT compound.
Date published: 2018-09-18
Rated 5 out of 5 by from Great conjugated antibody! I ordered the 633-conjugated antibody and it worked well. I used for staining but signal is less than original antibody with secondary antibody respectively.
Date published: 2018-08-09
Rated 5 out of 5 by from great antibody for IF of mouse cilia Very good staining of cilia, including kinocilia of mouse hair cells. I used it at 1:300 and 1:400 on PFA fixed cochlear whole mounts.
Date published: 2018-07-23
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