acetylated alpha Tubulin Antibody (6-11B-1)
  • acetylated α Tubulin Antibody (6-11B-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
  • raised against the outer arms of Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
  • recommended for detection of acetylated α Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P); non cross-reactive with non-acetylated α Tubulin or other lysine acetylation sites
  • available conjugated to HRP (sc-23950 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-23950 PE) or fluoroscein (sc-23950 FITC), Alexa Fluor® 488 (sc-23950 AF488) or Alexa Fluor® 647 (sc-23950 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
  • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of acetylated α Tubulin (6-11B-1): sc-23950.

acetylated α Tubulin Antibody (6-11B-1): sc-23950

acetylated α Tubulin Antibody (6-11B-1) is rated 4.9 out of 5 by 24.
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Datasheets
    • acetylated α Tubulin Antibody (6-11B-1) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against the outer arms of Strongylocentrotus purpuratus (Sea Urchin) sperm axonemes
    • recommended for detection of acetylated α Tubulin of mammalian species, zebrafish, Drosophila and Xenopus origin by WB, IP, IF and IHC(P); non cross-reactive with non-acetylated α Tubulin or other lysine acetylation sites
    • available conjugated to HRP (sc-23950 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; and to either phycoerythrin (sc-23950 PE) or fluoroscein (sc-23950 FITC), Alexa Fluor® 488 (sc-23950 AF488) or Alexa Fluor® 647 (sc-23950 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of acetylated α Tubulin (6-11B-1): sc-23950.
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
acetylated α Tubulin Antibody (6-11B-1) sc-23950 200 µg/ml $279.00
acetylated α Tubulin Antibody (6-11B-1) Alexa Fluor® 488 sc-23950 AF488 200 µg/ml $314.00
acetylated α Tubulin Antibody (6-11B-1) Alexa Fluor® 647 sc-23950 AF647 200 µg/ml $314.00
acetylated α Tubulin Antibody (6-11B-1) FITC sc-23950 FITC 200 µg/ml $292.00
acetylated α Tubulin Antibody (6-11B-1) HRP sc-23950 HRP 200 µg/ml $279.00
acetylated α Tubulin Antibody (6-11B-1) PE sc-23950 PE 200 µg/ml $303.00

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acetylated alpha Tubulin Antibody (6-11B-1) Product Citations

See how others have used acetylated alpha Tubulin Antibody (6-11B-1). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 44 total

PMID: # 27807597
Noack, K. et al. 2017. Analysis of the interplay between all-trans retinoic acid and histone deacetylase inhibitors in leukemic cells. Archives of toxicology. 91: 2191-2208.

PMID: # 28271209
Ehnert, S. et al. 2017. TGF-β1 impairs mechanosensation of human osteoblasts via HDAC6-mediated shortening and distortion of primary cilia. Journal of molecular medicine (Berlin, Germany). 95: 653-663.

PMID: # 27939219
Basak, O. et al. 2017. Induced Quiescence of Lgr5+ Stem Cells in Intestinal Organoids Enables Differentiation of Hormone-Producing Enteroendocrine Cells. Cell Stem Cell. 20: 177-190.e4.

PMID: # 26775640
Lienlaf, M. et al. 2016. Essential role of HDAC6 in the regulation of PD-L1 in melanoma. Mol Oncol. 10: 735-50.

PMID: # 27512957
Lee, JK. et al. 2016. Oncogenic microtubule hyperacetylation through BEX4-mediated sirtuin 2 inhibition. Cell Death Dis. 7: e2336.

PMID: # 27062996
Chu, C.W. et al. 2016. Prickle3 synergizes with Wtip to regulate basal body organization and cilia growth. Scientific reports. 6: 24104.

PMID: # 27270424
Sun, H. et al. 2016. The FEN1 L209P mutation interferes with long-patch base excision repair and induces cellular transformation. Oncogene.

PMID: # 26760506
Zhou, T. et al. 2016. R152C DNA Pol β mutation impairs base excision repair and induces cellular transformation. Oncotarget. 7: 6902-15.

PMID: # 26549550
Du, DS. et al. 2016. Effects of CDC42 on the proliferation and invasion of gastric cancer cells. Molecular medicine reports. 13: 550-4.

PMID: # 25666610
Bailey, JK. et al. 2015. WD repeat-containing protein 5 (WDR5) localizes to the midbody and regulates abscission. The Journal of biological chemistry. 290: 8987-9001.

Citations 1 to 10 of 44 total

Hi, I am interested in co-staining all microtubules (a-tubulin ab) and acetylated ones (acetylated a-tubulin). The issue is that both are mouse. Would a mouse secondary detect a conjugated acetylated a-tub antibody?

Asked by: JuanManuelGomez
Thank you for your question. We would not recommend co-staining with one conjugated primary antibody, and one un-conjugated primary antibody combined with an anti-mouse secondary antibody. There would still be the possibility of cross-reactivity between the anti-mouse IgG and the conjugated mouse monoclonal antibody. Instead, we would recommend co-staining with two conjugated primary antibodies, if both are mouse IgG.
Answered by: Technical Support
Date published: 2017-05-16

Dear Santa Cruz! I would like to stain the primary cilium of HK2 cells with sc-23950. I saw a cytoskeletal network on one picture and individual cilia on the other. How can I achieve the cilia staining, with no visibility of the cytoskeleton? Thank you

Asked by: mkeszthelyi
Thank you for your question. We do not have a specific protocol for using our monoclonal acetylated α Tubulin Antibody (6-11B-1): sc-23950 for detection of individual cilium exclusively. Provided below is a link to our recommended protocol for immunofluorescence and immunoperoxidase staining. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining This antibody has also been cited in the literature at least 30 times, many of which are using the antibody for IF or IHC applications. For example, PMID's: 27062996, 26079437 and 25286302. We hope that some of these publications may be useful in identifying a protocol for your research needs.
Answered by: Technical Support
Date published: 2017-03-01

What is the acetylation site that is recognized by this antibody?  Will it detect non-acetylated α Tubulin?

Asked by: Dr Ninau Qelp
acetylated α Tubulin Antibody (6-11B-1): sc-23950 recognizes endogenous levels of α-tubulin protein only when acetylated at Lys40.
Answered by: Tech Service 2
Date published: 2016-12-15

What is the recommended antigen-retrieval for IHC?

Asked by: Dr Ninau Qelp
We recommend 10 mM sodium citrate buffer, pH 6.0 and heating for 5 minutes at 95° C.
Answered by: Tech Service 2
Date published: 2016-12-15
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Rated 5 out of 5 by from great antibody 1:100 diluted 6-11B-1 antibody stained full differentiated airway epithelial cells, I got very good ciliated cell stain.
Date published: 2017-09-22
Rated 5 out of 5 by from indirect ELISA Used in an indirect ELISA to generate standard curve in order to determine VSX1 antibody titer in mouse serum.Diluted in a 1:500 it does the job really well.
Date published: 2017-08-19
Rated 5 out of 5 by from Works great for iummnohistochemistry Strong signal using 1:500 dilution on formalin-fixed mouse brain tissue
Date published: 2017-08-14
Rated 5 out of 5 by from acetylated α Tubulin Antibody (6-11B-1) acetylated α Tubulin Antibody (6-11B-1) showed a clear band for Western Blot and really defined IF images.
Date published: 2017-08-02
Rated 5 out of 5 by from It is nice This antibody is nice to stain spindle in mouse oocyte.
Date published: 2017-07-24
Rated 5 out of 5 by from strong band This antibody analysis in WB ,got strong band by 1:1000.
Date published: 2017-05-24
Rated 5 out of 5 by from great antibody This antibody works well in western blotting and immunostaining for endogenous protein.
Date published: 2017-05-12
Rated 5 out of 5 by from Acetylated alpha tubulin Antibody (6-11B-1) I received a free sample of this antibody, the delivery was prompt. I used it at 1: 1000 for my western blots, its worked excellent without any background or extra bands. I have used LICOR secondary antibody 1:5000.
Date published: 2017-05-11
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