ZFYVE27 Double Nickase Plasmid (h): sc-407267-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ZFYVE27 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ZFYVE27 Double Nickase Plasmid (h) and ZFYVE27 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ZFYVE27. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ZFYVE27 Double Nickase Plasmid (h) | sc-407267-NIC | 20 µg | $410.00 | |||
ZFYVE27 Double Nickase Plasmid (h2) | sc-407267-NIC-2 | 20 µg | $410.00 |
ZFYVE27 (also known as protrudin) encodes a FYVE domain–containing protein that binds phosphatidylinositol 3-phosphate and associates with endosomal membranes to coordinate membrane trafficking. It participates in endosome-to-plasma membrane transport, neurite outgrowth, and microtubule-dependent vesicle dynamics through interactions with Rab GTPases, kinesin motors, and ER–endosome contact site components. By coupling endosomal sorting with polarized transport, ZFYVE27 influences neuronal morphogenesis and axonal maintenance pathways. Dysregulation of ZFYVE27-linked trafficking has been connected to neurodegenerative phenotypes, including hereditary spastic paraplegia–related mechanisms, making it relevant for studying axonopathy and intracellular transport defects.
ZFYVE27 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ZFYVE27 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ZFYVE27. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ZFYVE27 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ZFYVE27-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.