UBE2E3 CRISPR/Cas9 KO Plasmid (m): sc-423576
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- UBE2E3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the UBE2E3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
UBE2E3 CRISPR/Cas9 KO Plasmid (m) | sc-423576 | 20 µg | $397.00 |
Overview
Ube2e3 encodes the mouse ubiquitin-conjugating enzyme UBE2E3, an E2 component of the ubiquitin–proteasome system that transfers ubiquitin from E1 enzymes to E3 ligases to regulate protein turnover and signaling. Through ubiquitination, UBE2E3 contributes to proteostasis, stress responses, and control of cell-cycle and DNA damage–associated pathways by shaping substrate stability and downstream transcriptional programs. As a modulator of protein quality control and regulatory ubiquitin signaling, altered UBE2E3 activity can influence cellular homeostasis in contexts relevant to neurobiology, inflammation, and cancer-associated phenotypes. Studying UBE2E3-dependent ubiquitination networks helps define how E2 enzymes tune pathway dynamics and substrate specificity across tissues.
UBE2E3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ube2e3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ube2e3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ube2e3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish UBE2E3 protein expression.
This CRISPR knockout system enables efficient generation of Ube2e3-deficient cell models for investigation of UBE2E3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ube2e3 exon(s) critical for UBE2E3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ube2e3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by UBE2E3 CRISPR/Cas9 KO Plasmid (m) and UBE2E3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ube2e3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by UBE2E3 HDR Plasmid (m) and UBE2E3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ube2e3 homology arms to support homology-directed repair at defined Ube2e3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.