TMPRSS11E CRISPR/Cas9 KO Plasmid (h): sc-412014
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TMPRSS11E CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the TMPRSS11E genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TMPRSS11E CRISPR/Cas9 KO Plasmid (h) | sc-412014 | 20 µg | $397.00 |
Overview
TMPRSS11E encodes a type II transmembrane serine protease expressed at epithelial surfaces, where pericellular proteolysis can regulate mucosal barrier function, cell–cell interactions, and remodeling of the extracellular microenvironment. As part of the TTSP family, TMPRSS11E is positioned to influence protease-activated signaling and proteolytic processing events that shape epithelial differentiation and inflammatory responses. Dysregulated epithelial protease activity has been associated with airway and squamous epithelia pathobiology, making TMPRSS11E a relevant target for studying mechanisms underlying epithelial stress responses and tissue remodeling. Its membrane-tethered protease architecture supports investigation of compartmentalized protease networks at the cell surface and their impact on downstream signaling programs.
TMPRSS11E CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the TMPRSS11E gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the TMPRSS11E together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the TMPRSS11E open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish TMPRSS11E protein expression.
This CRISPR knockout system enables efficient generation of TMPRSS11E-deficient cell models for investigation of TMPRSS11E signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting TMPRSS11E exon(s) critical for TMPRSS11E function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple TMPRSS11E genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by TMPRSS11E CRISPR/Cas9 KO Plasmid (h) and TMPRSS11E CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the TMPRSS11E locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by TMPRSS11E HDR Plasmid (h) and TMPRSS11E HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by TMPRSS11E homology arms to support homology-directed repair at defined TMPRSS11E target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.