Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Thrombospondin 5 CRISPR/Cas9 KO Plasmid (h): sc-402797
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Thrombospondin 5 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Thrombospondin 5 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Thrombospondin 5 Antibody (F-7): sc-374660
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Thrombospondin 5 CRISPR/Cas9 KO Plasmid (h) | sc-402797 | 20 µg | $397.00 |
Overview
COMP encodes thrombospondin 5, a pentameric extracellular matrix glycoprotein highly enriched in cartilage and tendon that supports matrix assembly and tissue biomechanics. Thrombospondin 5 interacts with collagens, proteoglycans, and cell-surface receptors to modulate chondrocyte adhesion, mechanotransduction, and extracellular matrix organization. It contributes to cartilage homeostasis by influencing collagen fibrillogenesis and matrix turnover pathways that shape skeletal development and joint integrity. Dysregulated COMP function and matrix deposition are associated with inherited skeletal dysplasias and degenerative cartilage phenotypes, making it a useful node for studying musculoskeletal extracellular matrix biology.
Thrombospondin 5 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the COMP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the COMP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the COMP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Thrombospondin 5 protein expression.
This CRISPR knockout system enables efficient generation of COMP-deficient cell models for investigation of Thrombospondin 5 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting COMP exon(s) critical for Thrombospondin 5 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple COMP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Thrombospondin 5 CRISPR/Cas9 KO Plasmid (h) and Thrombospondin 5 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the COMP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Thrombospondin 5 HDR Plasmid (h) and Thrombospondin 5 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by COMP homology arms to support homology-directed repair at defined COMP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.